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Biomedical subjects

P Boute

Publications and source records attributed to P Boute.

13 recordsLinked to original sources
PubMed1995

Quantitative determination of immunologic components of salivary gland secretion in long-term, event-free pediatric oncology patients.

OBJECTIVES: Chemotherapy influences the human immune system. Salivary alterations occur during cancer treatment. In this article we examine the salivary immunoglobulin content in pediatric patients who were long-term event-free and correlate these findings with different oral factors. STUDY DESIGN: Fifty-two children of a study group and 63 children of a control group were examined at our University Hospital. Caries prevalence and plaque index were scored. Whole saliva samples were taken for determination of slgA and IgG concentrations and Streptococcus mutans and Lactobacillus counts. For statistical analysis the Dental Survey Plus (Providence Software Services, Bristol, England) software package was used. RESULTS: Concentrations of slgA and IgG were within normal limits in both groups; slgA level increased with age. A negative correlation between slgA concentration and caries experience was not found in all age groups. No correlation with other salivary parameters was found. CONCLUSION: Salivary content of slgA and IgG can return to normal after cytotoxic therapy in these children. Salivary IgA seem to play a role in the development of dental caries in this population.

Adolescent↗
PubMed1995

Dental caries, gingival health, and oral hygiene of long term survivors of paediatric malignant diseases.

Fifty two children who had had cancer and been treated with chemotherapy, and who were long term event free, were examined for caries prevalence, gingival health, and oral hygiene and compared with a control group. A higher dental caries prevalence for the 14-17 year age group was noted. The restorative index was significantly lower in the age group 10-13. There were no significant differences in gingival index, plaque index, or toothbrushing frequency. It is concluded that these patients should be considered as at high risk for caries after cancer treatment. Professional dental follow up should be integrated in the medical follow up.

Adolescent↗
PubMed1993

[Preparation of bonded porcelain inlays and onlays using a milling-coping system].

The Celay-system offers an interesting alternative to existing systems for esthetic posterior restorations with ceramics. The basic idea of the concept is to prepare, either directly on the patient or indirectly on a plaster model, a pro-inlay using a light-curable precision material. The modelling also covers the occlusal surface. This pro-inlay is then used with the help of a milling center to machine a precise replica out of a ceramic block. The copying process is carried out via three-dimensional mechanical scanning and milling using eight axes of freedom. In this way additional corrections on the ceramic within the mouth can almost completely be avoided. The system is designed to be used as a direct chairside method by the dentist or as an indirect method by the dental technician. It is a promising but unproved restoration, more research and long-term clinical evaluation is recommended.

Adhesives↗
PubMed1989

Rheological properties of topical fluoride gels.

The rheological properties of several commercial topical fluoride gels were studied. For that purpose, we investigated hysteresis loops under standard conditions, equilibrium values, apparent viscosities as a function of shear rate, rate of thixotropic recovery, and the influence of temperature. The rheological equilibrium values, treated by the Power Law and the Cross Equation, show very important differences. Five gels show pseudoplastic behavior; seven have thixotropic properties. For the risks of fluoride gel toxicity to be lowered, high pseudoplasticity seems most desirable for the first group; for the thixotropic gels, a combination of yield value (very high viscosity at rest), the presence of a static yield value (very fast decrease in viscosity at very low shear rates), and both fast and high thixotropic recovery after destruction seems ideal. Positive and negative, as well as practically no, influence on rheological properties can be noted when the temperature changes.

Fluorides, Topical↗
PubMed1989

[The dilemma of the 2d primary molar].

Extensive carious lesions in primary molars often confront the dentist with a dilemma: extraction or restoration. The primary molar constitutes an important element in the development of the dentition. If extraction is considered, the dentist should be aware of the possible risks towards malocclusion or malposition. In order to understand these consequences a brief summary of normal dentition development is given, followed by a description of factors influencing development of dentition after premature extraction of a second primary molar. Premature extraction causes a disturbance in the eruption of the successor and migration of the neighbouring teeth. The eruption of the bicuspid can be delayed or accelerated according to the stage of root formation. The rate, amount and direction of migration depends on the extracted element, time of loss, spacing or crowding, eruption sequence, dental relationship, intercuspation, interaction of soft tissues and dental arch, supra-occlusion and the leeway space. To conclude the treatment modalities of pulp pathologies in primary molars are outlined. In children with deep carious lesions a treatment plan involves the child's medical history and social development as well as orthodontic, preventive and restorative aspects.

Child↗
PubMed1987

Effect of different combinations of calcium, magnesium and phosphate on the inorganic composition of rat molars in vitro.

Second upper molars from 3-day-old rats were cultured by the Trowell method for 14 days. One of each pair of molars was kept as an uncultured control; the other was cultured. Explants were exposed to eight different combinations of Ca, Mg and P additions to BGJb medium. This resulted in eight groups of explants (control, Ca, Mg, P, CaMg, CaP, PMg and CaMgP) and their eight uncultured contralateral groups. The additions were calculated to double the original measured media concentration. Cultured and uncultured germs were analysed for dry weight (D), ash weight (A), Ca, Mg and P content. The organic fraction (D-A) was calculated. The analysis of covariance by means of multiple regression revealed that Ca-addition to the culture medium stimulated D, A, Ca and P in the explants; P-addition was stimulatory for D, A, D-A and P whereas Mg addition was inhibitory for A, D-A and Ca. A positive interaction for all the tooth-germ variables was demonstrated after CaMg addition; an antagonistic effect was found for the tooth-germ variables D, A, Ca and P after CaP addition. The value of the tooth-germ variables at the time of explantation (covariate) had no significant effect on the value for the variables of the explants (except on their P content). The highest absolute values for all the variables were obtained after CaMg and CaMgP additions. Furthermore, taking into consideration morphological results, the addition of CaMgP can be recommended as medium supplement in the organ culture of rat tooth germs.

Animals↗
PubMed1987

Immunocytochemical localization of osteocalcin in developing rat teeth.

Osteocalcin was purified by gel chromatography from a crude extract obtained after decalcification of rat incisors. The apparent molecular weight, as determined by 5-15% SDS-polyacrylamide gel electrophoresis, was 18,000, and amino acid analysis revealed 60 gamma-carboxyglutamic acid residues per 1000. Antisera against osteocalcin, raised in rabbits, reacted specifically with osteocalcin when investigated by immuno-electroblotting of dentin crude extract. 4-micron cryosections of formaldehyde-fixed tooth germs showed positive immunocytochemical staining for osteocalcin in dentin and odontoblasts. The staining of the mantle dentin at the coronal sides of the tooth germs was more intense than that of the adjacent circumpulpal dentin, while the odontoblasts involved in the formation of mantle dentin showed stronger immunoreactivity than did odontoblasts involved in circumpulpal dentin formation. This marked difference was not observed on the root sides of the tooth germs. In 1-micron cryosections, osteocalcin immunoreactivity was found evenly distributed throughout the entire cell body, with the exception of the Golgi region, which was less intensely stained, while the nucleus and the cell process were negative. The positive staining reaction with anti-osteocalcin antiserum was found in dentin from the very onset of its formation in the fetus. In conclusion, our results demonstrate the presence of osteocalcin in odontoblasts and dentin. Its immunocytochemical localization may be compatible with a distinct role in early dentinogenesis.

Animals↗
PubMed1986

Characterization and immunocytochemical localization of dentine phosphoprotein in rat and bovine teeth.

Dentine phosphoprotein (DPP) was isolated from unerupted bovine molars and from rat incisors. The proteins were characterized biochemically and used to immunize rabbits and guinea pigs. Antibody activity was investigated by enzyme-linked immunosorbent assay (ELISA). Guinea-pig anti-rat DPP did not cross-react with bovine DPP, but rabbit anti-bovine DPP did cross-react with rat DPP. Anti-rat DPP antiserum was applied to cryotome sections of rat molar tooth germs and DPP immunoreactivity was seen in dentine, odontoblasts, odontoblast processes and pre-ameloblasts. Anti-bovine DPP antiserum reacted positively in bovine dentine and dentinal tubules. When this antiserum was applied to rat tissue, predentine was positive but dentine was negative. Adsorption experiments with DPP, purified by methods including and excluding precipitation with calcium, suggested that non-calcium precipitable DPP is present in rat predentine. Rat and bovine DPP are thus species-specific and DPP is synthesized by the odontoblasts, transported through their processes and secreted into the dentine.

Amino Acids↗
PubMed1986

Characteristics of mineralization of rat molar tooth germs in organ culture.

The behaviour of first (M1) and second (M2) upper molars of 3-day-old rats was compared histologically and by measuring several variables (dry and ash weight, Ca, P and Mg content) on cultured teeth and contralateral control teeth (not cultured). New information was gained by additional computations and calculating correlation coefficients between the variables of the control and cultured molars separately and combined. The M2 seems to perform better in culture than the M1. The M2 model revealed possibilities for further standardization.

Animals↗