Biomedical subjects

P C Bourdon

Publications and source records attributed to P C Bourdon.

10 recordsLinked to original sources

PubMed2003

Effect of measuring blood lactate concentrations using different automated lactate analysers on blood lactate transition thresholds.

This study investigated the effect of using three automated blood lactate analysers (Accusport, Lactate Pro, YSI 1500 Sport) on blood lactate transition thresholds (BLTT). Blood lactate concentrations were measured using the three analysers in rowers (n = 17) and kayakers (n = 6) during incremental exercise. The BLTT determined were: 1) ADAPT lactate threshold (data point preceding lactate increase of > or = 0.4 mmol x l(-1)), 2) log-log lactate threshold (point of lactate increase when log lactate plotted against log of relevant exercise parameter), 3) DMAX anaerobic threshold, 4) ADAPT anaerobic threshold (modified DMAX method), 5) Onset of blood lactate accumulation (OBLA, fixed blood lactate concentration of 4 mmol x l(-1)). Measurements of blood lactate concentration differed between analysers (p < 0.0001), resulting in BLTT differing between analysers when expressed as a blood lactate concentration (p < 0.0001), or when the BLTT was defined as a fixed blood lactate concentration (e.g. OBLA) (p < 0.0001). When expressed as a power output or heart rate using BLTT based on relative changes in lactate concentration (log-log, ADAPT and DMAX thresholds) the values were similar between analysers (p > 0.05), except the Accusport provided higher values for the log-log lactate threshold (p < 0.0001). We concluded that, despite providing significantly different lactate concentrations, unless the Accusport was used to determine the log-log lactate threshold, or values were expressed as a blood lactate concentration, the use of different analysers had little effect on the BLTT.

Adult↗

PubMed1999

Skin-prick blood samples are reliable for estimating Hb mass with the CO-dilution technique.

Investigation of the impact of environmental stimuli such as altitude exposure on hemoglobin mass currently rely on invasive techniques that require venous blood sampling. This study assessed the feasibility of lancet skin pricks as an alternative to venepuncture to estimate hemoglobin mass with the carbon monoxide (CO) dilution technique, with the intent of making the technique accessible to technicians without phlebotomy training. Sixteen healthy volunteers rebreathed CO via a small-volume rebreathing apparatus. Blood was sampled simultaneously with a glass syringe (VEN) from a superficial forearm vein and with a capillary tube from either a lanced fingertip or earlobe (CAP). As a control, VEN blood was then aliquoted into capillary tubes (CONTROL-CAP). Samples were assayed for carboxy-hemoglobin (HbCO) using a diode-array spectrophotometer. Mean %HbCO was higher in CAP than VEN (bias 0.3+/-0.2%HbCO, p < 0.01), but VEN and CONTROL-CAP were not different (p = 0.55). Compared to VEN, Hb mass derived from CAP samples was overestimated by 1.7% (15+/-22 g Hb, p = 0.01). CAP samples to estimate Hb mass demonstrated a technical error of measurement of 2.7%, which is comparable to the 1.9% reported previously with VEN samples. We conclude that using CAP samples gives a reliable measure of %HbCO, and will make the estimation of Hb mass with the CO-technique accessible to technicians without phlebotomy training.

Administration, Inhalation↗

PubMed1999

Effect of pedal cadence on the accumulated oxygen deficit, maximal aerobic power and blood lactate transition thresholds of high-performance junior endurance cyclists.

In this study we investigated the effect of pedal cadence on the cycling economy, accumulated oxygen deficit (AOD), maximal oxygen consumption (VO2max) and blood lactate transition thresholds of ten high-performance junior endurance cyclists [mean (SD): 17.4 (0.4) years; 183.8 (3.5) cm, 71.56 (3.75) kg]. Cycling economy was measured on three ergometers with the specific cadence requirements of: 90-100 rpm for the road dual chain ring (RDCR90-100 rpm) ergometer, 120-130 rpm for the track dual chain ring (TDCR120-130 rpm) ergometer, and 90-130 rpm for the track single chain ring (TSCR90-130 rpm) ergometer. AODs were then estimated using the regression of oxygen consumption (VO2) on power output for each of these ergometers, in conjunction with the data from a 2-min supramaximal paced effort on the TSCR90-130 rpm ergometer. A regression of VO2 on power output for each ergometer resulted in significant differences (P<0.001) between the slopes and intercepts that produced a lower AOD for the RDCR90-100 rpm [2.79 (0.43) l] compared with those for the TDCR120-130 rpm [4.11 (0.78) l] and TSCR90-130 rpm [4.06 (0.84) l]. While there were no statistically significant VO2max differences (P = 0.153) between the three treatments [RDCR90-100 rpm: 5.31 (0.24) l x min(-1); TDCR120-130 rpm; 5.33 (0.25) 1 x min(-1); TSCR90-130 rpm: 5.44 (0.27) l x min(-1)], all pairwise comparisons of the power output at which VO2max occurred were significantly different (P<0.001). Statistically significant differences were identified between the RDCR90-100 rpm and TDCR120-130 rpm tests for power output (P = 0.003) and blood lactate (P = 0.003) at the lactate threshold (Thla-), and for power output (P = 0.005) at the individual anaerobic threshold (Thiat). Our findings emphasise that pedal cadence specificity is essential when assessing the cycling economy, AOD and blood lactate transition thresholds of high-performance junior endurance cyclists.

Adolescent↗

PubMed1997

Reduced performance of male and female athletes at 580 m altitude.

This study examined the effect of mild hypobaria (MH) on the peak oxygen consumption (VO2peak) and performance of ten trained male athletes [x (SEM); VO2peak = 72.4 (2.2) ml x kg(-1) x min(-1)] and ten trained female athletes [VO2peak = 60.8 (2.1) ml x kg(-1) x min(-1)]. Subjects performed 5-min maximal work tests on a cycle ergometer within a hypobaric chamber at both normobaria (N, 99.33 kPa) and at MH (92.66 kPa), using a counter-balanced design. MH was equivalent to 580 m altitude. VO2peak at MH decreased significantly compared with N in both men [-5.9 (0.9)%] and women [-3.7 (1.0)%]. Performance (total kJ) at MH was also reduced significantly in men [-3.6 (0.8)%] and women [-3.8 (1.2)%]. Arterial oxyhaemoglobin saturation (SaO2) at VO2peak was significantly lower at MH compared with N in both men [90.1 (0.6)% versus 92.0 (0.6)%] and women [89.7 (3.1)% versus 92.1 (3.0)%]. While SaO2 at VO2peak was not different between men and women, it was concluded that relative, rather than absolute. VO2peak may be a more appropriate predictor of exercise-induced hypoxaemia. For men and women, it was calculated that 67-76% of the decrease in VO2peak could be accounted for by a decrease in O2 delivery, which indicates that reduced O2 tension at mild altitude (580 m) leads to impairment of exercise performance in a maximal work bout lasting approximately 5 min.

Adult↗

PubMed1995

Influence of test duration and event specificity on maximal accumulated oxygen deficit of high performance track cyclists.

This study examined the relationship between the time required to fully utilise the maximal accumulated oxygen deficit (MAOD) and event specificity of track cyclists. Twelve track endurance and 6 sprint high performance track cyclists performed four treatments of 70 s, 120 s, 300 s and 115% VO2max of maximal cycling on an air-braked ergometer. Peak blood lactate was measured immediately after each test with VO2 kinetics being assessed during the 115% VO2max time to exhaustion test. When the two cycling groups were combined there was no significant difference in the MAOD when assessed under the four different exercise durations. However, when the groups were analysed separately the following results were apparent: (1) the sprint cyclists achieved a significantly greater MAOD (66.9 +/- 2.2 ml.kg-1) compared to the track endurance cyclists (57.6 +/- 6.7 ml.kg-1) when a 70 s test duration was employed (2) even though the track endurance cyclists achieved their greatest MAOD during the 300 s test protocol (62.1 +/- 11.0 ml.kg-1), it was not significantly different to the MAOD's measured during the three other test durations and (3) the sprint cyclists recorded their greatest MAOD during the 70 s supramaximal test protocol (66.9 +/- 2.2 ml.kg-1). This was not significantly different to the 120 s test MAOD, but it was significantly higher than the MAOD values recorded during the 115% VO2max and 300 s test durations. There was no significant difference between the two groups in the peak post-exercise blood lactate concentrations for any of the tests and only the 70 s test produced a significant correlation between peak blood lactate and the MAOD. The VO2 kinetics (VO2 t1/2) of the sprinters was significantly slower than that of the track endurance cyclists (26.3 +/- 2.3 vs 23.9 +/- 2.8 s.). The findings of this study demonstrate that sprint cyclists can fully express their anaerobic capacity within an event specific 70 s all-out test and that these cyclists progressively decrease their anaerobic capacity during a 120 s, 115% VO2max (mean time = 210 s) or 300 s test, despite giving all-out efforts. Conversely, track endurance cyclists achieve their highest mean score during an event specific 300 s test and their lowest during a 70 s test. These findings have important implications when testing high performance cyclists for determination of MAOD, with the implication that it is necessary to assess MAOD under exercise conditions (i.e., duration, pacing) specific to the cyclist's chosen event.

Adolescent↗

PubMed1993

Aerobic and anaerobic indices contributing to track endurance cycling performance.

A group of 18 male high performance track endurance and sprint cyclists were assessed to provide a descriptive training season specific physiological profile, to examine the relationship between selected physiological and anthropometric variables and cycling performance in a 4000-m individual pursuit (IP4000) and to propose a functional model for predicting success in the IP4000. Anthropometric characteristics, absolute and relative measurements of maximal oxygen uptake (VO2max), blood lactate transition thresholds (Thla- and Th(an),i), VO2 kinetics, cycling economy and maximal accumulated oxygen deficit (MAOD) were assessed, with cyclists also performing a IP4000 under competition conditions. Peak post-competition blood lactate concentrations and acid-base values were measured. Although all corresponding indices of Thla- and Th(an),i occurred at significantly different intensities there were high intercorrelations between them (0.51-0.85). There was no significant difference in MAOD when assessed using a 2 or 5 min protocol (61.4 vs 60.2 ml.kg-1, respectively). The highest significant correlations were found among IP4000 and the following: VO2max (ml.kg-2/3.min-1; r = -0.79), power output at lactate threshold (Wthla) (W; r = -0.86), half time of VO2 response whilst cycling at 115% VO2max (s; r = 0.48) and MAOD when assessed using the 5 min protocol (ml.kg-1; r = -0.50). A stepwise multiple regression yielded the following equation, which had an r of 0.86 and a standard error of estimate of 5.7 s: IP4000 (s) = 462.9 - 0.366 x (Wthla) - 0.306 x (MAOD) - 0.438 x (VO2max) where Wthla is in W, MAOD is in ml.kg-1 and VO2max is in ml.kg-1 x min-1.(ABSTRACT TRUNCATED AT 250 WORDS)

Acid-Base Equilibrium↗

PubMed1993

Involuntary dehydration during cricket.

The sweat rate, heart rate and core temperature as well as urinary volume, osmolarity, electrolyte concentration and pH of 20 cricketers were measured under cool, warm and hot conditions with wet bulb globe temperature indices of 22.1, 24.5 and 27.1, respectively. Simulated match conditions were used on the cool and warm days, while 3 bowlers were measured under actual match conditions on the hot day. The tendency for higher heart rate, sweat rate and renal conservation of water and sodium on the warm day compared with the cool day is consistent with increasing thermoregulatory stress under relatively moderate environmental conditions. The average dehydration of the three fast bowlers was -4.3% of initial body mass after only two sessions of play, on the hot day. This level of dehydration is sufficient to impair physical performance. These results suggest that the adverse effects of dehydration could be minimised if the rules of cricket were amended to allow players the opportunity to drink as desired when the environmental conditions are extreme.

Adipose Tissue↗

PubMed1989

Spirometric standards for healthy male lifetime nonsmokers.

The FVC, FEV1.0, FEF25-75%, and FEV1.0/FVC (%) were measured in 162 males aged 18.9-78.6 yr using a Stead-Wells spirometer. Multiple regression equations were generated to predict these lung parameters from the best weighted combination (p less than or equal to 0.05) of age, standing height, sitting height, biacromial breadth, and chest expansion (FVC: R = 0.843, SEE = 513 ml BTPS; FEV1.0: R = 0.850, SEE = 436 ml BTPS; FEF25-75%: R = 0.665, SEE = 997 ml/s BTPS; FEV1.0/FVC: R = 0.537, SEE = 4.95%). The lower limit of normality was defined as the predicted value minus the 95% confidence interval (one-tailed test). Cross-validation of other FVC, FEV1.0, and FEF25-75% equations in the literature indicate that all the FVC and FEV1.0 ones are unsuitable for the sample and instrumentation used in this investigation.

Adult↗

PubMed1988

Lung volume standards for healthy male lifetime nonsmokers.

Functional residual capacity (FRC), residual volume (RV), vital capacity (VC), and total lung capacity (TLC) were measured in 162 men aged 18.9 to 78.6 years using a multiple breath helium dilution technique. Multiple regression equations were generated to predict these lung parameters from the best weighted combination (p less than or equal to 0.05) of age, mass, standing height, body surface area, sitting height, biacromial breadth, end-tidal chest girth, expanded chest girth, and chest expansion (FRC: R = .748, SEE = 504 ml; RV: R = .725, SEE = 301 ml; VC: R = .808, SEE = 537 ml; TLC: R = .808, SEE = 551 ml; RV/TLC: R = .778, SEE = 4.15 percent). The range of normality was defined as the predicted value +/- the 95 percent confidence interval (two-tailed test). Cross-validation of other FRC, RV, VC, and TLC equations in the literature indicated that they were unsuitable for use with our data.

Adolescent↗

PubMed1987

Relative body fat and anthropometric prediction of body density of male athletes.

Two hundred and seven male members of South Australian representative squads in 18 sports (mean +/- s = 24.2 +/- 4.7 years) were tested in order to provide descriptive data on relative body fat (% BF), develop a population specific equation and cross-validate existing equations. Measurements were taken of 10 circumferences, 2 diameters and 8 skinfolds; body density (BD) was measured by underwater weighing with the residual volume (RV) being determined by He dilution. The overall mean BD was 1.0761 g X cm-3 (s = 0.0085 g X cm-3; range = 1.0465-1.0968 g X cm-3) which corresponded to 10.0% BF according to Siri (s = 3.7%; range = 1.3-23.0%). The games players (n = 129) registered an overall mean of 10.3% BF (s = 3.7%; range = 2.2-23.0%). There were significant differences (p less than 0.05) for % BF between the lacrosse players (mean = 12.3%) an both the Australian Rules footballers (mean = 8.0%) and track and field athletes (mean = 8.7%). A stepwise multiple regression on 185 subjects yielded the following equation, which had an R of 0.787: BD = 1.078865-0.000419 (sigma abdominal, medial calf, front thigh and juxta-nipple skinfolds in mm) +0.000948 (neck circumference in cm) -0.000266 (age in decimal years) -0.000564 (ankle circumference in cm). Only those predictors which resulted in a significantly increased correlation (p less than or equal to 0.05) were included. The standard error of estimate of 0.00537 g X cm-3 was equivalent to 2.3% BF at the mean. This equation was satisfactorily cross-validated against the BD of a separate sample (n = 22) from the same population. However, cross-validation of 11 previously published equations indicated that they have limited applicability to State representative sportsmen.

Adipose Tissue↗