Biomedical subjects
Publications and source records attributed to P C Wong.
A new fluorimetric method to assay HGPRT (hypoxanthine-guanine phosphoribosyl transferase, EC 2.4.2.8) from human red cell lysates using 6MP (6-mercaptopurine) as substrate is described. This assay is compared with an existing spectrophotometric method using hypoxanthine as substrate. Precision, sensitivity limits and kinetic differences of these assays are reported.
Explore the source record for details and available documents.
1. Radioactivity was found to be associated with purine ribonucleotides in extracts of gravid adult Angiostrongylus cantonensis from the lungs of rats when the parasite was incubated in a medium containing [14C]glycine. 2. The rate of incorporation was at least 0.2 pmole/min per mg of parasite and approximately equal amounts of adenine and guanine ribonucleotides were synthesized. 3. This is the first demonstration of the capacity for a mammalian parasitic helminth to synthesize purines de novo.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The biochemical properties of partially purified preparations of human myocardial MB and MM iosenzymes of creatine kinase have been compared with one another. Differences in substrate affinity, behaviour with inhibitors, and heat denaturation have been demonstrated. It is doubtful if the differences we have shown are sufficiently great to form the basis of a practical routine procedure for measuring serum levels of the MB isoenzyme.