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P Cherubino

Publications and source records attributed to P Cherubino.

At least 19 recordsLinked to original sources
PubMed2003

Autologous chondrocyte implantation using a bilayer collagen membrane: a preliminary report.

OBJECTIVE: To present preliminary clinical experience with Matrix-induced autologous chondrocyte implantation, a new tissue-engineering technique for treatment of deep cartilage defects, in which autologous chondrocytes are seeded on a tridimensional scaffold provided by a bilayer type I-III collagen membrane. METHODS: From December 1999 to January 2001, 13 patients underwent implantation procedure for deep cartilage defects. Age of patients ranged from 18 to 49 years (mean age, 35 years). The mean defect size was 3.5 cm(2) (range, 2.0-4.5 cm(2)). Clinical and functional evaluation were performed using various score systems for the ankle and the knee, and magnetic resonance imaging was performed at 6 and 12 months postoperatively. Membrane structure and cellular population were investigated by light microscopy, scanning electron microscopy, and electrophoresis before implantation. RESULTS: The mean follow-up was 6.5 months (range, 2-15 months). No complications were observed in the postoperative period. The 6 patients with a minimum follow-up of 6 months showed an improvement in clinical and functional status after surgery. Magnetic resonance images showed the presence of hyaline-like cartilage at the site of implantation; there was evidence of chondroblasts and type II collagen inside the seeded membrane. CONCLUSION: Matrix-induced autologous chondrocyte implantation offers several advantages with respect to the traditional cultured cell procedure. These include technical simplicity, short operating time, minimal invasiveness, and easier access to difficult sites. It appears to be a reliable method for the repair of deep cartilage defects.

Adolescent↗
PubMed2001

Preliminary results of a survey of the use of antimicrobial agents as prophylaxis in orthopedic surgery.

An epidemiological survey of the use of antimicrobial prophylaxis in Italian hospitals was carried out under the auspices of the Journal of Chemotherapy. Out of 500 Italian orthopedic centers queried, 225 agreed to participate in this study. A total of 136,321 surgical procedures were reported in the 166 centers reporting complete answers on type of surgery. They comprised hip and knee prosthesis (13.9%), spine surgery (4%), hip endoprosthesis (5.2%), osteosynthesis (26.9%), arthroscopy (24.4%), and others (25.5%). Perioperative antimicrobial prophylaxis was used in 75% of operations (ranging from 57.1% to 99.4% in arthroscopy and joint prosthesis, respectively). Short term (<24 h) antimicrobial prophylaxis was performed in 38.4% of the 206 centers answering this question correctly. 61.1% of centers employed single agent prophylaxis and 70.8% of these prescriptions were betalactam antibiotics. Bacteriological analysis revealed gram-positive isolates in 73.3% of cases. Methicillin resistance was present in 45% of 915 tested strains. Out of 4221 patients with high risk of infectious complications (joint prosthesis surgery) given antimicrobial prophylaxis in 46 centers, the percentage of surgical wound infections was overall 2.1%, while that of non-surgical wound infections was 3.6%. The total infection rate was decreased by about half in association with long-term (>24 h) as compared to short-term (<24 h) antibiotic treatment (3.7% vs 7.6%, respectively), and with use of antibiotic drug combinations vs single antibiotic drugs (3.9 vs 6.6%, respectively). The incidence of surgical-site infection was not decreased by extending the chemoprophylaxis for more than the first 24 h after surgery, while it was reduced from 2.5 to 1.4% by use of combination antibiotic therapy.

Aged↗
PubMed2000

[Antibiotic prophylaxis of postoperative infection in orthopedics. Results of an epidemiologic survey in Italy conducted by the Journal of Chemotherapy].

An epidemiological survey of the use of antimicrobial prophylaxis in Italian hospitals was carried out under the auspices of the Journal of Chemotherapy. Out of 500 Italian orthopedic centers requested, 225 have participated in this study. A total of 136,321 surgical procedures were reported in the 166 centers reporting complete answers on type of surgery. They comprised hip and knee prosthesis (13.9%), spine surgery (4%), hip endoprosthesis (5.2%), osteosynthesis (26.9%), arthroscopy (24.4%), and others (25.5%). Perioperative antimicrobial prophylaxis was used in 75% of operations (ranging from 57.1% to 99.4% in arthroscopy and joint prosthesis, respectively). Short term (< 24 h) antimicrobial prophylaxis was performed in 38.4% of the 206 Centers answering this question correctly. 61.1% of Centers employed single agent prophylaxis. 70.8% of these prescriptions were betalactam antibiotics. Bacteriological analysis of the wound in 86 Centers (total number of isolates 2013) revealed the presence of Gram-positive isolates in 73.7% of cases. Methicillin resistance was expressed in 45% of 915 tested strains. Out of 4221 patients with high risk of infectious complications (joint prosthesis surgery) given antimicrobial prophylaxis in 46 Centers, the percentage of surgical wound infections was overall 2.1%, while that of non-surgical wound infections was 3.6%. A decrease in the total infection rate by about half was associated with long-term (> 24 h) as compared to short-term (< 24 h) antibiotic treatment (3.7 vs 7.6%, respectively), and with the use of antibiotic drug combinations vs single antibiotic drugs (3.9 vs 6.6%, respectively). The incidence of surgical-site infection is not decreased by extending the chemoprophylaxis for more than the first 24 h after surgery, while it is reduced from 2.5 to 1.4% by use of combination antibiotic therapy.

Antibiotic Prophylaxis↗
PubMed1998

Treatment of closed humeral shaft fractures with intramedullary elastic nails.

PURPOSE: Our purpose is a retrospective study of the results of diaphyseal humeral fracture treatment by elastic intramedullary nailing in comparison with plate osteosynthesis. METHODS: Thirty cases were reviewed, with an average follow-up of 2 years. In 14 cases, flexible nails were used, and in the remaining 16 cases, A-O plates were used. We considered patients' sex and age, fracture type according to the A-O classification, type of treatment, consolidation defects, additional therapeutic procedures, healing time, and functional recovery. The surgical technique of elastic nailing appeared very simple, safe, and rather atraumatic because the nail is introduced in a retrograde manner and does not need proximal interlocking. RESULTS: The results of elastic nailing, in terms of fracture healing time and functional recovery, appeared comparable with the results of plating, and complications appeared milder. CONCLUSION: We conclude that intramedullary elastic nailing is valid for routine use in appropriately selected humeral shaft fractures.

Adolescent↗
PubMed1995

A diagnostic workstation for neoplastic bone marrow diseases: evaluation on 526 cases.

The diagnostic accuracy of a workstation for neoplastic bone marrow pathology was evaluated on 526 cases from two institutions. The workstation consists of knowledge-based systems for peripheral blood analysis, flow cytometry immunophenotyping, and bone marrow morphology, linked together by a relational database. The "gold standard" diagnosis was established by complete agreement among three hematopathologists after independent review of the data from each case. The workstation's diagnosis agreed with the "gold standard" in 515 cases (97.9%). In six of the 11 diagnostic errors, the bone marrow module could not reach a final diagnosis. The five misclassifications would not have resulted in a change of therapy for the patient. The institutional diagnosis of record agreed with the gold standard in 462 cases (87.8%). The results indicate that the workstation is capable of high-quality, reproducible, multiparameter interpretive reporting.

Acute Disease↗
PubMed1993

Experimental model in vivo for quantitative assessment of bone resorption inhibition.

Quantitative assessment of bone resorption inhibition in vivo is not easily accomplished; methods relying on a count of osteoclasts are questionable, and histomorphometric evaluation of the bone mass presents several technical problems as well. The authors developed a simple method to measure the inhibition of bone resorption by study of the proximal tibial metaphysis of growing rats: the height of the perichondrial bone ring was taken as an index of the balance between osteoblastic and osteoclastic activity because any agent that inhibits osteoclasts (without interference with osteoblasts) produces an increase in the height of this anatomical structure. Since the ring is well demarcated by surrounding tissues, its height can be measured with accuracy and used for quantitative assessment of bone resorption inhibition. This model was tested with salmon calcitonin, and it provides evidence in vivo that this hormone inhibits osteoclastic bone resorption.

Animals↗
PubMed1993

Evaluation of hollow and full stems implanted in the rabbit tibia: preliminary results.

Full and hollow stems were implanted in the right and left tibiae of five rabbits to study the effects of the implant shape on revascularization and remodelling of the bone after 6 months. No significant difference of periosteal and endosteal circumference was observed when the left and right tibiae of each group were compared. Medullary area was, not surprisingly, significantly smaller in the full stem implanted tibiae. The cortical bone area result was significantly higher in the hollow stem group. In hollow stems, vessels were present both inside and outside the implant with connections through the holes of the implant. The observed differences of cortical bone area can be explained in terms of increased vascular spaces inside the cortical bone of full stem implanted tibiae. This supports the hypothesis that hollow implants provide more space for medullary revascularization and are consequently capable of inducing less endosteal remodelling. Cementless prostheses rely for mechanical fixation on a large interface of the implant with bone. Two factors, i.e. revascularization of the medullary canal and stiffness of the stem, can have a relevant effect on the quality of interface. This non-weight-bearing model suggests the relevance of vascular factors.

Animals↗
PubMed1991

Osteochondroma of the cervical spine.

The localization of an osteochondroma in the spine is considered a rare occurrence. The authors describe the case of a 37 year-old woman with osteochondroma of the C2 neural arch involving the C1 spinous process. The woman came under observation because of severe loss of neck mobility and was subsequently diagnosed with osteochondroma. Of the preoperative examinations, MRI was more valuable than CT scan for defining the nature and extent of the tumor. Treatment consisted of complete posterior excision of the tumor, including the C1 spinous process and the C2 laminae up to the facets.

Adult↗
PubMed1990

Degenerative arthritis of the adjacent spinal joints following anterior cervical spinal fusion: clinicoradiologic and statistical correlations.

On the premise that cervical intersomatic spinal fusion in the treatment of traumatic or spondylolytic myelopathy and post-traumatic instability provokes degenerative arthritis due to functional overloading of the spaces adjacent to the fusion, 37 patients (average age 57, range 25-80) who had undergone spinal fusion by Cloward's technique were subjected to radiographic study and clinical evaluation. The average follow-up was 11.5 years (range 7-16 years). In comparison with a control group of the same age who had not undergone this operation, these patients' C3-C4 and C6-C7 spaces (adjacent to the most commonly fused vertebrae) were smaller by 16.7% and 15.9%, respectively. In spite of the worsening of the radiographic situation, from a clinical standpoint there was a significant improvement in the symptomatology of 86.5% of the patients. Thus the degenerative arthritis is both less evident and, as far as the long-term clinical outcome is concerned, less influential than could be expected. This finding is confirmed by the lack of statistical correlation between the clinical results and the radiographic evidence.

Adult↗
PubMed1989

Study of the osteoconductive properties of bioactive glass fibers.

Bioactive glass fibers have been prepared and implanted in cortical defect and in muscle. The fibers can act as a substrate for bone apposition, when implanted in a cortical defect, and become incorporated in the new bone matrix. The same results were obtained when fibers were implanted in a muscle pouch together with bone marrow cells. An intense inflammatory reaction was observed when bioactive glass fibers were implanted in muscle; the reaction was milder when fibers were implanted in bone or in muscle together with bone marrow cells. This fact supports the hypothesis that osteogenic cells adhere in an early phase to the substrate and prevent recognition of the foreign material by inflammatory cells. This appears to be a fundamental condition for direct bone matrix apposition on the surface of fibers.

Animals↗
PubMed1989

[Surgical treatment of complex lesions of the wrist].

The authors underline the importance of the precocious surgical treatment of the complex lesions of the carpal bones, especially in case of concomitant neurological lesions. The quality of the late results depends on the careful anatomical reconstruction and on the fixation during a suitable period with the aim to obtain a satisfactory repair of the carpal ligaments.

Carpal Bones↗
PubMed1988

Primary bone tumours of the hand. Report of 21 cases.

Twenty-one primary bone tumours of the hand in children from 8 paediatric hospitals are reported. Osteochondromas and enchondromas were not included. Our material consisted of 16 patients with common tumours (3 Ewing's sarcoma, 5 aneurysmal bone cyst, 6 osteoid osteoma and 2 epidermoid cyst) and 5 patients with uncommon tumours (osteoma, simple bone cyst, haemangiopericytoma, capillary angiomatous tumour and benign ossifying fibroma or osteoblastoma). The X-ray diagnosis of the common tumours should have high concordance with histology, whereas that of uncommon tumours is much more difficult and uncertain. The characteristic features of Ewing's sarcoma are stressed as all our children with this tumour had a delayed diagnosis and a fatal outcome. Differential diagnosis with other short tubular bone lesions of the hand - specifically osteomyelitis - is discussed and the possibilities of microscopic diagnosis are stressed.

Adolescent↗
PubMed1988

Isoelastic shoulder prostheses. Considerations on use and results.

The principles underlying the use of isoelastic shoulder prostheses are discussed and the results obtained in 8 patients are reported. Two operations were for pseudarthrosis of the humeral neck and 6 for osteolytic neoplasms. The results were assessed after an average follow-up of 4 years. Full movement was never recovered; there was partial recovery in 6 cases, and no significant recovery in 2. The extent of the surgical resection was determined by the type of lesion; in more conservative resections which allowed for the reconstruction of tendinous insertions, the functional results were better than in wide resections. The difficulty of recovering good movement was related to the limited possibilities of reconstructing the insertions of the external rotator muscles.

Adult↗