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Biomedical subjects

P Chiewsilp

Publications and source records attributed to P Chiewsilp.

At least 19 recordsLinked to original sources
PubMed1992

A new type of hepatitis C virus in patients in Thailand.

Partial nucleotide sequences in the tentative NS5 region of the hepatitis C viral genome obtained from patients with chronic hepatitis in Thailand were analyzed by reverse transcription followed by the polymerase chain reaction. Of ten samples studied, four showed low homologies to any known type of HCV: the homologies of the nucleotide sequences of these clones with HCV-J, -US, -K2a and -K2b were 66.5-69.1%, 66.5-68.2%, 61.2-64.1% and 64.4-66.2%, respectively, and the homologies of their deduced amino acids sequences were 71.7-75.2%, 71.7-75.2%, 69.0-72.6% and 69.9-73.5%, respectively. These four clones were classified a new distinct type of HCV, named HCV-T. Moreover, the nucleotide and amino acid sequence homologies of the four HCV-T clones showed that the HCV-T type could be classified into two genotypes, HCV-Ta and HCV-Tb.

Amino Acid Sequence↗
PubMed1992

Autologous blood donation for elective gynaecological surgery.

The utilization and effectiveness of a hospital autologous blood donation programme for elective gynaecological surgery at Ramathibodi Hospital during April 1, 1988-March 31, 1991 were analyzed. A total of 438 patients or 98% of eligible patients were enrolled into the programme. Each patient gave 1 unit of blood within 14 days of undergoing gynaecological surgery. The untoward effects observed in the programme were higher than those found in the regular donors. However, these were very mild. Nearly 90% of the participants used no homologous blood during their hospitalization, 8% used only their autologous blood, while 2% used additional homologous transfusion. The released autologous components were subsequently administered to other recipients. The patients' acceptance was excellent. Autologous blood donation is a safe, inexpensive and feasible procedure for transfusion treatment in elective gynaecological surgery especially in developing countries.

Blood Specimen Collection↗
PubMed1992

Bone marrow transplantation in Ramathibodi Hospital: progress report.

Bone marrow transplantation has become the accepted treatment for several hematologic disorders. We have done 3 autologous and 6 allogeneic bone marrow transplantations at Ramathibodi Hospital since July 1989 in patients with acute lymphoblastic leukemia, acute non-lymphocytic leukemia, chronic myeloid leukemia, non-Hodgkin's lymphoma and severe aplastic anemia. Only one patient with aplastic anemia had late graft rejection, but the rest of them engrafted and did well during the median follow up period of 317 days (range: 39 to 962 days) post transplantation. None of the allogeneic BMT had graft-versus-host disease. We use cyclosporin and short course methotrexate for post transplantation immunosuppression.

Adolescent↗
PubMed1992

Antibodies to hepatitis C virus among patients with hepatocellular carcinoma and blood donors in Thailand.

HCC is the most cancer among Thai men. It is not known if HCV plays an oncogenic role in HCC in this country where HBV is endemic. Anti-HCV and HBsAg were assayed in 154 sera from HCC and 3,387 voluntary blood donors. The prevalence of anti-HCV in HCC (8.4%) was significantly higher than blood donors (1.38%). The prevalence of HBsAg in HCC (61%) was also significantly higher than blood donors (5.28%). The prevalence of anti-HCV in HCC was lower than that of Spain, Italy, Africa and Taiwan. Anti-HCV was found associated with a small portion of patients with HCC while HBV was found closely associated with the larger proportion of HCC. HCV in normal Thais was as common as those in southern Europe and HCV was found associated with HCC. However, HBV remains the major etiological factor of HCC in Thailand.

Adult↗
PubMed1992

Fetal red cell staining: method evaluation.

Acid elution methods have been extensively used for the identification of fetal red cells in pregnant women with fetomaternal hemorrhage. A number of methods have been described: Kleihauer-Betke, Boehringer Mannheim, Fetaldex, Nierhaus-Betke modification and Sanguansermsri amido black B. A comparative evaluation of these methods was performed in this study. The technique of Sanguansermsri using amido black B was found to be more advantageous than the others in terms of simplicity, accuracy, precision and time taken.

Erythrocyte Count↗
PubMed1992

Anti-HIV seroconversion in hemophilia A patient after transfusion of anti-HIV negative blood components.

A Thai male hemophiliac A patient has been anti-HIV seroconverted in the hospital after a 2-year admission for a large infected retroperitoneal hematoma. The source of HIV is thought to be one or more of more than 20,000 units of blood components used during the admission. All were screened as anti-HIV negative. Although he received heat treated factor VIII concentrate before this admission, it was not thought to be the cause. Autologous blood transfusion and HIV antigen screening are suggested as a safer way of blood transfusion.

Adult↗
PubMed1991

Diffuse systemic sclerosis and related diseases in Thailand.

Diffuse systemic sclerosis and the related syndromes were diagnosed in 115 patients during 1977-1987. Sixty-nine patients were available for clinical evaluation and analysis. These included 46 patients with diffuse systemic sclerosis, 2 with limited cutaneous systemic sclerosis, 2 with linear scleroderma, 18 with overlap syndrome (OS), and one with primary Raynaud's disease. The major clinical manifestations among the diffuse scleroderma patients were the involvement of the following systems; cutaneous (93.3%), musculoskeletal (69.6%), gastrointestinal (54.3%), and pulmonary (43.3%) while renal involvement (4.3%) was less common. Antinuclear antibodies were found in 89.7% with predominating speckle pattern (86.2%) and anti Scl 70 antibody (40.6%). In contrast to the Western series, HLA-DR2 was significantly increased in our diffuse systemic sclerosis patients (p less than 0.01). Among the 18 OS patients, the involvement of pulmonary system (44.4%) was the major cause of morbidity and renal involvement (55.6%) was found frequently although it was clinically mild. In conclusion, our diffuse systemic sclerosis and related diseases patients seem to have milder clinical manifestations than those of the Western series.

Adult↗
PubMed1990

Prevention of hepatitis B infection in infants born to hepatitis B carrier mothers: low dosage vaccination.

Two groups of newborn infants born to HBeAg positive carrier mothers were given HBIG (200 IU) immediately after birth. Subsequently, at age 2 days and at 1, 2 and 12 months, the first group received 5 microgram and the second group 2 microgram of HBV vaccines. There was no significant difference in the anti-HBs seroconversion rate (SR), and the protective efficacy rate (PER) at the age of 13 months in either group. The SR and PER of group 1 were 91.7% and 90.18%, and group II were 92.9% and 91.01%, respectively. Although the significant differences were observed in the geometric mean titers of anti-HBs in group I (526.3 mIU/ml) and group II (371.4 mIU/ml), both were above the protective level. The immune responses to the reduced dosage of HBV vaccines are satisfactory in preventing HBV in the newborn infants of HBeAg positive carrier mothers.

Female↗
PubMed1989

Long-term immunoprophylaxis of hepatitis B surface antigen carrier in infants born to hepatitis B surface antigen positive mothers using plasma derived vaccine.

Immunoprophylaxis of HBV infection using 3 doses (10 micrograms) of plasma derived HBV vaccine were given to 49 newborn infants of e-antigen negative HBs Ag carrier mothers at birth, one and 6 months of age. The protective efficacy rate was 100% during first 2 years. At 3 years of age, HBs Ag carrier was 2.63% and 89.48% of infants had anti-HBs above the protective level. Ten point fifty-two percent of total infants had anti-HBs level below 10 mIU/ml and 21.05% had anti-HBs level between 10-100 mIU/ml. Most infants who had the maximal level of antibody below 100 mIU/ml had lost all antibody within 3 years. The loss of antibody was closely related to the maximal anti-HBs titer (one year of age) which is highly predictive indicator of the longevity of anti-HBs.

Antibodies, Viral↗
PubMed1979

The HLA antigens and leprosy in Thailand.

HLA-A and -B locus antigens were determined in 170 unrelated patients with leprosy and 100 healthy controls. There were no statistically significant deviations from expected antigen distribution in patients with any of the various forms of leprosy, though the number of patients studied is relatively small. There were some interesting trends, particularly a possible decrease in the number of tuberculoid patients with A9, and an increase in the proportions of lepromatous patients with B18 and B40. Limited evidence of a genetically controlled pre-disposition to the clinical manifestations of leprosy suggests that further studies with extended typing should be carried out on the HLA haplotype segregation in informative leprosy families. These should provide a clearer indication of the relevance of the HLA (including DR) and possibly of other non-HLA loci to the possible mechanism(s) of disease susceptibility.

Adolescent↗
PubMed1978

Red cell ABH antigens in leukaemias and lymphomas.

The qualitative and quantitative determinations of A, B, and H antigens were studied in blood samples of 509 normal subjects and 114 patients. 11 of 53 (20.75%), 3 of 27 (11.11%), and 4 of 34 (11.76%) of adults with leukaemia, children with leukaemia and adults with lymphomas, respectively, show significantly lower antigenic scores than normal controls. Since all A, B and H antigens were found to be independently affected, the destruction of well-developed ABH antigens by a certain substance produced during the course of the disease was suspected.

ABO Blood-Group System↗
PubMed1976

The HL-A system in Thais.

The HL-A antigens were studied in 100 Thai subjects. HL-A2, 11 and 9 of the first series and W15, HL-A17, 5, and 13 of the second series were commonly found. HL-A2-11 and W15- were the commonest phenotypes observed in the first and the second series, respectively. The HL-A2, X2, and 2, W15 were the most common haplotypes. However, there was no significant gametic association.

Asian People↗