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P Craig

Publications and source records attributed to P Craig.

41 records · Page 3Linked to original sources
PubMedDate not supplied

Partial characterization of antigens in circulating immune complexes in cystic hydatid patients treated with albendazole.

Immune complexes were precipitated by 3% polyethylene glycol (PEG) from 4 sera from albendazole-treated hydatid patients from Uruguay, and together with sheep hydatid cyst fluid were analysed by ion-exchange fast protein liquid chromatography (FPLC). FPLC-eluted fractions of serum preparations and cyst fluid were tested in an enzyme-linked immunosorbent assay for the detection of specific Echinococcus granulosus antigens and also for presence of specific immunoglobulin (Ig) M and IgG in the serum samples. Two peaks (nos 13 and 25) were found to have antigenic activity. Antigens were identified in both serum and cyst fluid preparation (fraction 25) after sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting analysis, with approximate molecular masses 50 kDa and 62 kDa. Specific IgM antibody was also detected in the same FPLC fraction (no. 25) of PEG-precipitated serum complexes.

Adolescent↗
PubMedDate not supplied

Improved video documentation of endoscopic sinus surgery made possible with desktop digital video.

Endoscopic cameras in endoscopic sinus surgery (ESS) allow for easy video documentation of surgery. This often leads to the build-up of extensive videocassette libraries where the prospective viewer is subjected to a lengthy and cumbersome videotape search before accessing a sequence of interest. Footage is difficult to use for educational purposes. New techniques for storing, retrieving, and presenting ESS video footage were developed. Desktop computer systems were used to digitize video footage. Short video sequences containing highlights of interesting ESS cases were prepared using video-editing programs. The resulting images were stored in individual segments on recordable CD-ROM disks. This technique was used to archive all video footage from our ESS procedures for a 6-month period. Archiving footage on CD-ROM rather than videotape has made the storage of large quantities of information possible while now allowing for the random access of segment(s) of interest. It has simplified preparation of video projects, reduced production time 10-fold and has eliminated the need for professional video-editing services. These techniques have also been adapted for the development of an educational CD-ROM demonstrating the component steps of ESS, which can be played back on any CD-ROM-equipped computer.

CD-ROM↗