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Biomedical subjects

P D Amin

Publications and source records attributed to P D Amin.

6 recordsLinked to original sources
PubMed2002

A stability indicating LC method for felodipine.

A stability indicating reversed-phase liquid chromatographic (RP-LC) method was developed for the assay of felodipine as a bulk drug and in pharmaceuticals. The chromatography was performed on a C18 column. Eluents were monitored by UV detection at 238 nm using the mobile phase methanol-potassium dihydrogen orthophosphate (pH 3.5; 0.01 M) (75:25, v/v). The method was statistically validated for linearity, accuracy, precision and specificity. The linearity of felodipine peak area responses was demonstrated within the concentration range of 1-7 microg/ml. The limits of detection and quantitation were 150 and 500 ng/ml, respectively. The method was demonstrated to be precise, accurate and specific with no interference from the tablet excipients and separation of the drug peak from the peaks of the degradation products (oxidative degradation, photodegradation, acid and base degradation). The results indicated that the proposed method could be used in a stability assay.

Calcium Channel Blockers↗
PubMed2001

Sustained ophthalmic delivery of ofloxacin from a pH triggered in situ gelling system.

The poor bioavailability and therapeutic response exhibited by conventional ophthalmic solutions due to rapid precorneal elimination of the drug may be overcome by the use of in situ gel-forming systems that are instilled as drops into the eye and undergo a sol-gel transition in the cul-de-sac. The present work describes the formulation and evaluation of an ophthalmic delivery system of an antibacterial agent, ofloxacin, based on the concept of pH-triggered in situ gelation. Polyacrylic acid (Carbopol 940) was used as the gelling agent in combination with hydroxypropylmethylcellulose (Methocel E50LV) which acted as a viscosity enhancing agent. The developed formulation was therapeutically efficacious, stable, non-irritant and provided sustained release of the drug over an 8-h period. The developed system is thus a viable alternative to conventional eye drops.

Anti-Infective Agents↗
PubMed2001

Stability indicating HPTLC determination of Trimetazidine as bulk drug and in pharmaceutical formulations.

A simple, selective, precise and stability-indicating high-performance thin-layer chromatographic method of analysis of trimetazidine hydrochloride both as a bulk drug and in formulations is reported. The mobile phase composition was n-butanol-water-methanol-ammonia (20%) (14:0.2:0.2:2, v/v/v/v). Densitometric analysis of trimetazidine hydrochloride was carried out in the absorbance mode at 254 nm. the calibration curve of trimetazidine hydrochloride in methanol was linear in the range 400 -- 2400 ng. The mean value of correlation coefficient, slope and intercept were 0.99815 and #61617;0.001, 0.4849 and #61617;0.001 and 31.633 and #61617;5.996 respectively. The limits of detection and quantitation were 50 and 80 ng respectively. The recovery of trimetazidine hydrochloride was about 98 -- 100%. This method was utilized to analyze trimetazidine hydrochloride from conventional tablets and controlled release pellets in the presence if commonly used excipients.

Calibration↗
PubMed2001

Trimetazidine: stability indicating RPLC assay method.

An accurate, specific and reproducible reversed phase liquid chromatographic method for the determination of trimetazidine hydrochloride in presence of its degradation products is reported. The mobile phase consisted of water-acetonitrile-triethylamine (90:10:0.1, v/v/v) adjusted with o-phosphoric acid to a pH of 3.3. Chromatography was performed using C-18 column at a flow rate of 1.0 ml/min and the drug along with its degraded products was detected at 270 nm. The calibration curve of trimetazidine hydrochloride in methanol was linear in the range 500-3000 ng. The mean value of correlation coefficient, slope and intercept were 0.99859 &# 0.001, 17.7986 &# 0.0709 and 482.56 &# 147.03, respectively. The limits of detection and quantitation were 5 and 20 ng, respectively. The recovery of trimetazidine hydrochloride was about 99-100%. This method was utilized to analyze trimetazidine hydrochloride from conventional tablets and controlled release pellets in the presence of commonly used excipients.

Calibration↗
PubMed2000

Stability indicating HPTLC determination of timolol maleate as bulk drug and in pharmaceutical preparations.

Timolol was the first beta blocker to be used as an anti-glaucoma agent and to date remains as the standard because none of the newer beta blockers were found to be more effective. The high performance thin layer chromatographic method of analysis of timolol maleate is reported. The mobile phase selected was ethyl acetate-methanol-isopropyl alcohol-ammonia (25%) (80:20:2:1, v/v/v/v). The calibration curve of the drug was linear in the range of 100-600 ng. The spectrodensitometric analysis was carried out at 294 nm. The mean (+/- RSD) values of slope, correlation coefficient and intercept were 2487.5 (+/- 0.9), 0.996 (+/- 0.081) and 90463 (+/- 1.1), respectively. The system precision and the method precision were excellent with an RSD of 2.8 and 1.004, respectively. The limits of detection and quantitation were 10 and 40 ng, respectively. The mean percent recovery was found to be 98.6. Timolol maleate was degraded by exposing the drug to heat, acid and base. The degraded products were found to be well separated from the pure drug with significantly different Rf values suggesting a stability indicating analysis method for quantification of timolol maleate in pharmaceutical preparations and as bulk drug. The method was utilized to analyze timolol maleate from conventional eye drops and novel sustained release solid polymeric ocular inserts and oral preparations. The reported method is simple, selective, precise, accurate, time saving and economic as compared to reported HPLC methods.

Adrenergic beta-Antagonists↗