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Biomedical subjects

P Danesino

Publications and source records attributed to P Danesino.

7 recordsLinked to original sources

Radiographic evaluation of teeth subjected to high temperatures: experimental study to aid identification processes.

The radiographic evaluation of dental remains represents a significant aspect in the forensic identification process, particularly after an exposure to fire. The aim of this "in vitro" study was to evaluate the radiographic features of unrestored, endodontically treated and restored teeth after exposure to an experimental range of high temperatures. Ninety human teeth were divided into two groups: (1) unrestored teeth, as a control group and (2) teeth endodontically treated (condensation technique) and restored with amalgam or composite fillings. Before testing the high temperatures, periapical radiographs of all teeth were performed. The tests of exposure to heat were carried out in an oven for six different temperatures (200, 400, 600, 800, 1000 and 1100 degrees C (392, 752, 1112, 1472, 1832, 2012 degrees F)). After each exposure, periapical radiographs of all the teeth were taken. The radiographic appearance of all the teeth before and after the thermal stresses were evaluated and the differences were recorded. The results of the radiographic examination showed that a number of significant radiographic details were conserved: the composite fillings were in place maintaining the shape till 600 degrees C (1112 degrees F), the amalgam fillings were in place maintaining the shape till 1000 degrees C (1832 degrees F) and the endodontic treatments were recognisable till 1100 degrees C (2012 degrees F).

Case-Control Studies↗

Further study of restored and un-restored teeth subjected to high temperatures.

Forensic dentistry has been shown to be of fundamental importance in medico-legal investigations aimed at identifying human remains involving high temperature incidents because dental remains and prosthetic devices are resistant to quite high thermal change. In this project we studied teeth containing class I and V amalgam and composite fillings and compared them to un-restored teeth when exposed to high temperatures. Twenty five un-restored teeth, 25 teeth with class I amalgam restorations, 25 teeth with class V amalgam restorations and 25 teeth with class I composite fillings were placed in a furnace and heated at a rate of 30 degrees C/min. The effects at the predetermined temperatures 200, 400, 600, 800, 1000 and 1100 degrees C were examined macroscopically and then observed microscopically by means of a stereomicroscope. Our observations showed that the class I amalgam restorations at the different temperature levels remained in place, maintaining their shape despite disintegration of the crowns, whilst the class I composite restorations remained in place but in an altered shape. Comparing restored with un-restored teeth we observed different responses in crown disintegration at the different temperature levels.

Acrylic Resins↗

Observations on dental prostheses and restorations subjected to high temperatures: experimental studies to aid identification processes.

In large scale disasters associated with fire the damage caused by heat can make medico-legal identification of human remains difficult. Teeth, restorations and prostheses, all of which are resistant to even quite high temperatures can be used as aids in the identification process. In this project the behaviour and morphology of teeth and dental prostheses exposed to a range of high temperatures was studied. Healthy teeth, dental restorations and prostheses were placed in a furnace and heated at a rate of 30 degrees C/min and the effects of the predetermined temperatures 200, 400, 600, 800, 1000 and 1100 degrees C were examined by stereomicroscopy and scanning electron microscopy (SEM). Our observations show that some prostheses and restorative materials resist higher temperatures than theoretically predictable and that even when a restoration is lost because of detachment or change of state its ante-mortem presence can be confirmed and detected by both stereomicroscopic examination and SEM of the residual cavity. We further conclude that a reasonably reliable estimation of the temperature of exposure can be made from an analysis of the teeth and restorative materials.

Composite Resins↗

Fluorescent technique for macroscopic detection of electrical metallization.

A fluorescence method for the detection of trace metals was investigated to visualize in situ latent current marks or electrical metallic traces on cloth. The main electrodes metallization (iron, copper, zinc, aluminum, nickel, chromium, and brass) can be identified by spraying a 0.2% solution of 8-hydroxyquinoline in ethanol followed by observation under shortwave UV light. Advantages and disadvantages of this technique are compared with previously described methods.

Electric Injuries↗

The macroscopic detection of metallization in the latent current mark.

Immediate revelation of latent current mark (LCM) was investigated by means of macroscopic detection of metallization (M). LCM were produced on corpses using electrodes of metals most frequently involved in electrocution (iron, copper, zinc, aluminium, chrome, nickel, and brass). Also, current marks of accidental electrocutions (10 cases) were examined. Single metals can be identified by spraying specific reagents on the suspected skin. The following multiple systems can be used to simultaneously produce the above mentioned metals: A. Sequence: 10% ammonium sulphid--H2O--0.2% solochrome cyanine in 1% HCl. B. Sequence: 0.5% 2.nitroso.1.naphtol in acetone--H2O--0.2% solochrome cyanine in 1% HCl. Instead of appearing on the skin, metallization can sometimes be found on clothes or shoes. Macroscopic search for LCM metallization is a very simple method and it is recommended in both certain and suspected electrocution, as well as in cases of sudden, unexpected death, particularly during work.

Electric Injuries↗

[De Quervain's tenosynovitis and work with hand tools: a case study].

Work-related musculoskeletal disorders of the upper limbs are a frequent complaint and their pathogenetic mechanism is often related to mechanical overload of the body region involved without traumatic events. The occupational origin of such disorders is much debated, since the clinical pictures are often not specific and may be found in subjects who do not perform hazardous work tasks. A better understanding of the relationship between clinical symptoms, diagnosis, and functional capacity would provide a deeper knowledge in decision making for return to work and rehabilitation strategies. In this paper we present a case study of a worker using scissors who developed De Quervain's syndrome in the dominant hand. A methodology for the biomechanical investigation of the work task and the possible occupational aetiology of the clinical picture are discussed according to the findings of the specific literature.

Adult↗