Biomedical subjects

P Darbyshire

Publications and source records attributed to P Darbyshire.

At least 19 recordsLinked to original sources

PubMed1992

Rapid production of diversity during the progression of a mixed lineage leukaemia.

A leukaemia presenting with two morphologically different blast populations failed to respond to either antimyeloid or antilymphoid treatment and showed a rapid clinical progression. Immunophenotyping provided good evidence for two blast populations, one lymphoid and the other lymphoid with granulocyte monocytic markers. Two different gene rearrangements within JH were also observed with band densities corresponding to the sizes of the two blast cell populations. A t(19; 22) translocation was observed in almost all cells at presentation one of which evolved into a subclone, becoming dominant in the terminal phase of the disease. We show here both the clonal evolution and clonal competition that occurred in this leukaemia and suggest that the potential of the tumour stem line for rapidly producing diversity was the reason for the resistance to treatment.

Bone Marrow↗

PubMed1990

Sickle cell disease in the UK.

Explore the source record for details and available documents.

Anemia, Sickle Cell↗

PubMed1986

Idiopathic thrombocytopenia in childhood. Edinburgh experience 1962-82.

This retrospective review of 136 children with idiopathic thrombocytopenia assesses the prognostic significance of various presenting parameters and the effect of therapy. Initial haemoglobin, white cell count, actual platelet count, splenomegaly, hepatomegaly and lymphadenopathy had no significant effect upon the final outcome. Cases of acute I.T.P. were associated with a higher incidence of preceding infection, a shorter history of bleeding and a preponderance of males. The presence of greater than 20 per cent lymphocytes in the bone marrow was associated with a longer time to achieve a normal platelet count (p = 0.05). Steroid therapy shortened the time for acute cases of I.T.P. to obtain a normal platelet count (p = 0.05), but had no effect on long-term prognosis. Chronicity occurred in 25 children (18%) and nine of these had spontaneous remissions. Immunosuppressive therapy was ineffective, but nine out of 12 children were cured by splenectomy. Mortality was 0.7 per cent (one death) and general morbidity was low.

Acute Disease↗

PubMed1985

Lysosomal isoenzyme profiles used to classify a case of acute undifferentiated leukaemia.

Lysosomal enzyme activities and isoenzyme profiles were measured in lymphoid and non-lymphoid leukaemic cells from childhood patients. High activities, especially of beta-hexosaminidase and alpha-mannosidase, were associated with leukaemic cells of myeloid or monocytic origin. Leukaemic cells from two children with acute myeloid leukaemia had a relative reduction in the B isoenzyme of beta-hexosaminidase activity, whereas in patients with non T, non B cell acute lymphoblastic leukaemia, intermediate beta-hexosaminidase isoenzymes were expressed. A patient is described on whom conventional marker studies were either negative or equivocal, but lysosomal enzyme markers were consistent with a myeloid leukaemia. This observation was supported by the clinical course of this patient.

Child↗

PubMedDate not supplied