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P DenBesten

Publications and source records attributed to P DenBesten.

5 recordsLinked to original sources
PubMed2005

Mouse amelogenin exons 8 and 9: sequence analysis and protein distribution.

Amelogenin is the major protein of the developing enamel. Two additional exons, termed 8 and 9, have been characterized in the rat. Our aim was: to identify the mouse amelogenin exons 8/9 sequences; to investigate the potential presence of the alternative spliced isoforms of amelogenin exons 8/9; and to immunolocalize proteins containing sequences encoded by exons 8/9 during odontogenesis. RT-PCR analysis with exon 9 anti-sense primer generated 2 major amplicons with the use of a mouse tooth cDNA library and dental cell lines. DNA sequence analysis showed 93% identify with the rat exons 8/9 sequence. Alternative splicing of exon 3 was also found, but only in cDNAs lacking exons 8 and 9. Immunohistochemistry localized exons 8/9-encoded proteins in ameloblasts, young odontoblasts, and stratum intermedium cells. Analysis of our data supports the hypothesis that: (1) AMELX contains 2 additional exons; (2) ameloblasts and odontoblasts synthesize amelogenin 8/9; and (3) amelogenin splice variants may have unique functions during tooth formation.

Alternative Splicing↗
PubMed2002

Stem cell properties of human dental pulp stem cells.

In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.

Acid Phosphatase↗
PubMedDate not supplied

Fluoride levels in whole saliva of preschool children after brushing with 0.25 g (pea-sized) as compared to 1.0 g (full-brush) of a fluoride dentifrice.

Concerns regarding an increased risk of dental fluorosis related to ingesting fluoride-containing toothpastes by preschool children have led to recommendations to reduce the amount of toothpaste used for young children to a pea-sized amount. The purpose of this study was to determine the effect on salivary fluoride levels of reducing the amount of toothpaste used in a preschool-age (4-5 years) population. Salivary fluoride concentrations were determined for 10 children whose teeth were brushed with both 0.25 g and 1.0 g of a fluoridated toothpaste on two separate days. Initial salivary fluoride levels following the use of 0.25 g of toothpaste were less than half of the salivary fluoride concentrations when 1.0 g of toothpaste was used, and levels returned to baseline more rapidly. The reduced salivary fluoride levels when less toothpaste is used may result in a reduced efficacy for caries prevention. However, any potential reduction in caries prevention may still be outweighed by the risk of increased fluoride ingestion with larger amounts of toothpaste in preschool children. The results of this study suggest that a reduction in the amount of a fluoridated toothpaste to a pea-sized amount be limited only to young children who are at risk of ingesting toothpaste.

Cariostatic Agents↗