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P F Costa

Publications and source records attributed to P F Costa.

12 recordsLinked to original sources
PubMed2006

Potential bird dispersers of Psychotria in a area of Atlantic forest on Ilha Grande, RJ, Southeastern Brazil: a biochemical analysis of the fruits.

The present study assessed the fruiting pattern, bird foraging behavior, and sugar content of ripe fruits of two sympatric species of Rubiaceae (Psychotria brasiliensis and P. nuda). This study was carried out in an Atlantic forest area on Ilha Grande, RJ, between August 1998 and July 1999. Fruit production occurred year round, with a peak of mature P. brasiliensis fruits in December 1998 and another of P. nuda in February of 1999. Lipaugus lanioides (Cotingidae), Baryphtengus ruficapillus (Momotidae) and Saltator similis (Emberizidae) made the most frequent foraging visits to fruiting P. brasiliensis, so that L. lanioides and B. ruficapillus removed the fruits with sallying maneuvers while S. similis gleaned the fruits. Lipaugus lanioides was by far the most important consumer, and potentially the main disperser of P. brasiliensis. Birds of this genus are heavy frugivores in the tropical forests and are widely assumed to be important seed dispersers. The fruits were analyzed quantitatively and qualitatively in relation to the amounts of sucrose and starch. Psychotria brasiliensis (the visited species) showed the smallest quantity of sucrose and the highest amount of starch. These findings suggest that what may influence the birds' choice of fruit is the proportion of starch in the Psychotria species studied here rather than the carbohydrate composition.

Animals↗
PubMed2001

Sodium channel currents in maturing acutely isolated rat hippocampal CA1 neurones.

Sodium channel currents were recorded in excised inside-out patches from immature (P(4-10)) and older (P(20-46)) rat CA1 neurones. Channel conductance was 16.6+/-0.013 pS (P(20-46)) and 19.0+/-0.031 pS (P(4-10)). Opening patterns varied with step voltage and with age. In some patches bursting was apparent at voltages positive to -30 mV. Non-bursting behaviour was more dominant in patches from younger animals. In older animals mean open time (m.o.t.) was best described by two exponentials especially in the older cells; in the immature, there were fewer cases with two exponentials. The time constant of inactivation (tau(h)) estimated in ensemble averages was best described by two exponentials (tau(hf) and tau(hs)) in most patches from older cells. tau(hf) decreased with depolarization; tau(hs) increased in the range -30 to 0 mV. The voltage dependence of tau(hf) in the older cells is identical to that of the single tau(h) found in the younger; the results indicate a dominance of tau(hf) in the younger. Patches from younger cells more often showed one apparent active channel; in such cases, m.o.t. was described by a single exponential. However, in two cases, channels showed bursting behaviour with one of these channels showing a shift between bursting and non-bursting modes. Our findings are consistent with a heterogeneous channel population and with changes in the population in the course of maturation.

Animals↗
PubMed2000

Kinetic parameters of calcium currents in maturing acutely isolated CA1 cells.

Calcium currents were recorded in CA1 hippocampal cells from immature (P(4-10)) and older (P(22-55)) rats, using whole-cell voltage clamp techniques. Parameters defining the voltage-dependence of activation (tau(m)) and inactivation (tau(h)), steady-state inactivation and activation were determined at both stages of maturation. Current density increased with maturation. A transient low voltage activated (l.v.a.) current was found in P(4-10) cells, but not in the older cells. At voltages less negative than -30 mV, current inactivation was best described by two exponentials (tau(hf), tau(hs)); the ratio of the amplitudes of the two components changed with maturation, with a dominance of the faster component (tau(hf)) in the younger cells. The voltage dependence of tau(hf) followed a simple dependence model, decreased with increasing depolarization, in all cells at both stages of maturation. In P(4-10) cells, tau(hs) was voltage insensitive (range -25 to +30 mV); in P(22-55) cells, the voltage dependence of tau(hs) was found to be complex. Two current components were identified from the voltage dependence of the conductance in both groups. The first, more hyperpolarized component, the l.v.a. current found in P(4-10) cells; this was absent in the older cells, in which we found a component with a different voltage dependence. The voltage dependence of the conductance of the second, more depolarized component did not differ in younger and older cells. In the course of maturation, the steady-state inactivation of the second component underwent a hyperpolarizing shift and a decrease in voltage sensitivity.

Aging↗
PubMed1998

Sustained potassium currents in maturing CA1 hippocampal neurones.

Whole-cell voltage clamp techniques were used to characterize sustained outward currents in maturing (P4 to P48) acutely isolated rat CA1 hippocampal neurones. Sodium removal and signal subtraction were used to isolate a sodium dependent sustained potassium current (IKNa). Calcium blockade (Co2+), sensitivity to a low TEA dose (0.5 mM) and sensitivity to Charibdotoxin (CTX 25 nM) and Iberiotoxin (IbTX 25 nM), in conjunction with signal subtraction, were used to isolate a sustained current with the characteristics of IC (IKCa). IKNa was found in both immature (P4-5) and older (P > 21) cells; this corresponded, respectively, to 56 +/- 5% and 36 +/- 6% of the outward current in younger and older cells. In the course of maturation, the voltage dependence of activation of IKNa shifted to more hyperpolarized values by approximately 20 mV. In the younger cells (P5-18) there was no evidence for sensitivity to CTX or IbTX. In 55 out of 77 older cells we found a component sensitive to CTX, IbTX, 0.5 mM TEA and Co2+.

Animals↗
PubMed1996

The kinetic parameters of sodium currents in maturing acutely isolated rat hippocampal CA1 neurones.

Whole-cell voltage clamp techniques were used to characterize the kinetics of INa in immature (P3-5) and older (P > 25) acutely isolated rat CA1 hippocampal neurones. Fast-rising and fast-inactivating currents were recorded at all stages of maturation, evocable from Vm values of -55 to -50 mV. Currents were sensitive to TTX (1 microM) and to sodium removal from the perfusate. Current density and maximum slope conductance increased with maturation. Current decay was described by two exponentials, the faster component dominating at -35 mV or more depolarized Vm values; the ratio fast/slow inactivating component decreased with maturation. The voltage-dependence of conductance was taken as an approximation of m infinity. In younger cells, V1/2 values of the steady-state inactivation (h infinity) and activation curves (m infinity) were depolarized. Shifts of h infinity and m infinity curves were accompanied by shifts in the corresponding tau h and tau m voltage-dependence curves. In younger cells, activation curves had comparatively higher slope factors (Vs), which is an indication of a lower voltage sensitivity of activation. m infinity, tau m, h infinity, and tau h parameters were used to calculate the forward and backward activation and inactivation rate constants (alpha m, beta m, alpha h and beta h). P3-5 cells had relatively higher beta m values accounting for the lower voltage sensitivity of activation. The findings are an indication of a dominant channel variety in the younger cells with a closed state higher probability. The results are consistent with lower depolarization rates previously reported in CA1 cells at early stages of maturation. Faster inactivation due to poor expression of the slower inactivating component may compensate for poorer repolarization mechanisms due to the immaturity of outward currents previously reported at early stages of maturation.

Animals↗
PubMed1994

Potassium currents in acutely isolated maturing rat hippocampal CA1 neurones.

Whole-cell voltage clamp techniques were used to characterize the postnatal development of current amplitude and inactivation and activation kinetics of two potassium currents in acutely isolated CA1 cells from rats P4 to P52: the A-current (IA) and a slow-rising, slow inactivating current (IK). In the course of maturation, changes in the relative proportion of IA and IK currents were observed, the latter becoming a dominant current in older cells. The half-maximal point (Vh) of steady-state inactivation and activation of IA and IK shifted in the course of the first and second postnatal weeks. The shifts were hyperpolarizing in the case of IK, whereas IA shifted to less negative values. The shifts in steady-state inactivation Vh were accompanied by a change in the slope factor (Vs), which is an indication of a modification in the voltage sensitivity of the steady-state inactivation. The kinetics of IK evolve after birth in a fashion that matches the changes in action potential parameters previously reported.

Animals↗
PubMed1992

Slow and medium afterhyperpolarizations in maturing rat hippocampal CA1 neurones.

The postnatal evolution of the signals underlying the afterhyperpolarization (slow-AHP and medium-AHP) was studied in rat CA1 hippocampal neurones (P10-16, P17-23 and P greater than 26) using in vitro slices. Noradrenaline (NA) and signal subtraction were used to decompose the AHP (m-AHP and s-AHP). The amplitude of the s-AHP was found significant between P10-16 and P17-23 cells; the m-AHP showed no significant change. The time to peak of the s-AHP of the P greater than 26 cells was found to be significantly different from the remaining groups; the m-AHP showed no significant change. The changes in waveform and amplitude of the AHP at this stage were found to be mainly due to the modifications of the slow-AHP.

Aging↗
PubMed1991

Afterpotential characteristics and firing patterns in maturing rat hippocampal CA1 neurones in in vitro slices.

The postnatal evolution of depolarizing after-potentials (DAPs) and after-hyperpolarizations (AHPs) was studied in rat CA1 hippocampal neurones (5-68 days of age) using in vitro slices. Results were pooled into 4 age groups: P5-9, P10-16, P17-24 and P26-68. In P5-9 cells, DAPs were seen as passive signals, with a time constant similar to the time constant of the membrane. The evolution of the DAP was characterized by a decrease in amplitude, an increase in duration and a change in contour. In P10-16 and P17-24 cells, the DAPs often had a plateau or a hump-like shape which increased the probability of firing and the occurrence of spike doublets. The firing pattern and bursting behaviour of P10-16 CA1 neurones differed from the pattern typical of the adult. P5-9 and P10-16 cells had post-burst AHPs with a smaller amplitude and a more prolonged early phase than at late stages of development.

Action Potentials↗
PubMed1989

Determination of ionic permeability coefficients of the plasma membrane of Xenopus laevis oocytes under voltage clamp.

1. A method of estimating absolute ionic permeability coefficients which does not depend on the use of impermeant substitutes is reported. 2. The method is based on a pump leak model of the Xenopus laevis oocyte membrane. The procedure consists of measuring, in the same experiment, the pump current and the currents generated under voltage clamp by the partial substitution of one or two ions at a time. For each experimental condition, the measured currents are substituted in a Goldman-Hodgkin-Katz type equation with two unknowns (the permeability coefficients). The set of equations thus generated enables the computation of all the ionic permeability coefficients. 3. The Xenopus oocyte membrane (stages IV and V, Dumont, 1972) has been found to be permeable to conventional ion substitutes such as N-methyl-D-glucamine (NMG), sulphate, isethionate and gluconate. 4. The values for sodium, potassium and chloride permeability coefficients obtained from sixty-eight pooled experiments were, respectively, 5.44, 17.41 and 1.49 x 10(-8) cm s-1. 5. The diffusional currents for sodium, potassium and chloride computed from the experiments referred to above were, respectively, -1.16, 0.69 and -0.038 microA cm-2. 6. A stoichiometry of the Na+-K+ pump exchange of 3/1.8 was computed. 7. The intracellular concentrations of sodium, potassium and chloride ions, as determined by ion-selective microelectrodes, were, respectively, 10.1 +/- 0.66 mM (n = 12), 109.5 +/- 3.3 mM (n = 13) and 37.7 +/- 1.18 mM (n = 19), corresponding to equilibrium potentials of 61, -95 and -28 mV. 8. Since chloride is not at equilibrium across the membrane, we propose that there is an inward uphill Cl- transport.

Animals↗
PubMed1985

[The therapeutic approach to drug addicts].

Difficulties of biological, psychological, social and legal nature may be seen involved in the treatment of drug-addict persons. In this study, the major models used in the treatment are analysed, so as so one may arrived at a new model which will better integrate the various factors involved. The major models may be brought down to three: the medical psychiatric model which emphasizes the biological dependency on drugs and which equates drug addiction and "mental illness"; the behavioristic model which utilizes directive conditioning and desensitizing techniques, as well as educational and suggestional means, in order to determine new kinds of behavior; the relational model which takes origin from psychoanalysis and the systemic approach and sponsors a non-directive treatment of the drug-addict through the exploration and work on his personal and social unconscious conflicts, drive and desires, as well as his self-destructive tendencies. To assure the drug-addict a way to assume responsibility for his own behavior and to be free by respecting other people's rights, it is necessary to elaborate an integrative model of treatment which will consider also the anthropological specificity of the problems, referring also to the Brazilian society.

Behavior Therapy↗
PubMedDate not supplied

[Applications of problem-based learning in the basic medical curriculum].

This article describes a project, which took place between 1992 and 1994, on the application of problem-based learning to three disciplines of the basic medical curriculum: Biochemistry (1st year) Physiology (2nd year) and physiopathology (3rd year). The main objectives of the project were to bring teaching closer to the future professional reality, to stimulate critical thinking and to facilitate independent learning. Despite differences in content and in teaching materials, the trigger event for learning was information given within a clinical context which was further analyzed in small-group tutorial sessions. The project also included the training of the tutors and the development of audiovisual materials. Although no statistically significant differences were found in the final scores between students taught during this period and before, the first group reported a considerable increase in the motivation to learn which, in the case of Biochemistry, was important to change the passive attitude of most students on entering university. The project also helped to create the appropriate atmosphere for the establishment of an office for medical education in the medical school to support other innovations in the field. With this project we wished to contribute, with a concrete experience, to the reform of medical education in Portugal, by demonstrating that problem-based learning can be used in single disciplines without major changes in the basic curriculum.

Curriculum↗