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Biomedical subjects

P Földi

Publications and source records attributed to P Földi.

7 recordsLinked to original sources
PubMed1997

About streamlined brachial dialysis shunts.

The authors modified the technics of fistula forming. In such cases where to create a connection on the forearm is impossible one possible modality is to make it between major vessels on the arm. Streamlined connection was made in seven patients between basilic vein and brachial artery in three cases, between cephalic vein and brachial artery in four. There were two men and five women. The age interval was between 58 and 78 years. The longest observation period is 30 months. Until this time the fistulas were suitable for dialysis and had abundant yield. Because of the number of cases and the observation period has not yet make possible to come to final conclusion their goal was to present technical solution first of all. Their expectation is that with making them streamlined they will manage to create good perfusing and long lasting fistulas.

Aged↗
PubMed1985

An ultra rapid and sensitive high-performance liquid chromatographic method for determination of tissue and plasma free amino acids.

An ultra rapid and sensitive HPLC method for measuring individual free amino acids in biological fluids has been developed by using o-phthaldialdehyde/3-mercaptopropionic acid as derivatization agent and employing 3-micron-particle-size reversed-phase columns. Resolution of the amino acid derivatives is accomplished with an acetonitrile gradient in 12.5 mM sodium phosphate buffer, pH 7.2. These conditions facilitate separation of the 23 major tissue free physiological amino acids in the lower picomole range in less than 13 min. Muscle, liver, and kidney free amino acid concentrations, as determined by HPLC, are in the expected physiological range and compare favorably with those obtained by conventional amino acid analyzer.

Amino Acids↗
PubMed1984

Measurement of free amino acids in human biological fluids by high-performance liquid chromatography.

Favourable analytical conditions allowing amino acid analysis in biological fluids, acquired from small human biopsy specimens, were achieved by considering various derivatization methods, the mode of detection and the column used. By using o-phthalaldehyde-3-mercaptopropionic acid as derivatization agent (high sensitivity and stability) and fluorescence detection (excitation at 330 nm, emission at 450 nm), excellent separation of 26 amino acids was obtained in the lower pmol range (1-10 pmol). The reproducibility of the retention times was better than 1.0% for the majority of amino acids and the results from high-performance liquid chromatography (HPLC), compared favourably with those of conventional amino acid analysis (r = 0.97). HPLC technology facilitates amino acid analysis in biopsy specimens of less than 1 mg of tissue.

3-Mercaptopropionic Acid↗
PubMed1984

Increase of cloning efficiencies by using high-performance liquid chromatography-purified vectors and linkers.

High-performance liquid chromatography (HPLC) was applied to the purification of vector and linker DNAs used in molecular cloning experiments. The data clearly demonstrate that HPLC (Blue Column from LKB/TSK G 4000 SW) based on steric exclusion is a very rapid and convenient method for the size separation of DNA fragments that are virtually free of any contamination. Application of vector and linker DNAs, purified by this method, enabled us to clone nanogram amounts of avian myeloblastosis virus RNA, which was used for the evaluation of a recently described cloning procedure.

Animals↗
PubMedDate not supplied

The effect of repeated doses of (-) deprenyl on the dynamics of monoaminergic transmission. Comparison with clorgyline.

The action of clorgyline and (-)deprenyl on the dynamics of dopaminergic and serotonergic transmission in the rat brain was compared. It was found, that daily administration of 0.25 mg/kg sc clorgyline, a specific MAO A inhibitor, reduced the turnover rate of both dopamine and serotonin after two weeks of injections. The treatment for two or four weeks with 0.25 mg/kg sc (-)deprenyl, a specific MAO B inhibitor, enhanced the turnover rate of dopamine and the fractional rate constant of dopamine efflux, reflecting an increased utilization rate of this amine in the striatum. Beside the augmentation of the dopamine turnover rate, the dopaminergic tone was also elevated by the reduction of the dopamine uptake in the striatum. Two week injections with the same dose of (-)deprenyl did not change the dynamics of serotonergic transmission.

Animals↗