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Biomedical subjects

P G Fast

Publications and source records attributed to P G Fast.

13 recordsLinked to original sources
PubMed1990

An in vitro system for testing Bacillus thuringiensis toxins: the lawn assay.

A cell assay system was developed that allows Bacillus thuringiensis delta-endotoxins activated at high pH (10.5) to be tested in vitro without causing alkaline injury to target cells. The assay is carried out on a lawn of gel-suspended cells, requires only 1 microliter of sample per dose, and is quantitative, rapid, and sensitive. The threshold dose for toxicity of B. thuringiensis subsp. kurstaki HD-73 with IPRI-CF-1 cells was 24 pg protein. The assay is also very useful for identifying antibodies which inhibit toxicity and for detecting beta-exotoxin.

Animals↗
PubMed1978

Bacillus thuringiensis delta-endotoxin: evidence that toxin acts at the surface of susceptible cells.

Enzymically activated delta-endotoxin of Bacillus thuringiensis covalently bound to Sephadex beads, has the same effect on insect cells in tissue culture as free toxin. The effect is prevented by antitoxin antibody and heat denaturation and is not due to a nonspecific protein effect, the beads, or toxin released from the beads. The toxin, therefore, probably acts at the cell surface.

Antitoxins↗
PubMed1968

Studies of chlorophyll-lipid-water systems.

Preliminary studies of the electronic absorption spectra of chlorophyll molecules in aqueous glycolipid and phospholipid dispersions are reported. The spectra show a shift for the red peak of chlorophyll of some 10 millimicrons to longer wavelength compared with the spectrum of chlorophyll in ether. Photochemical studies were carried out with cytochrome c incorporated into the aqueous layers separating the lipid bilayers.

Chemical Phenomena↗
PubMed1967

Ethanolamine phosphoglycerides: effect on the properties of myelinoid lecithin water systems.

The amount of swelling solution trapped when mixtures of ethanolamine and choline phosphoglycerides were dispersed in 0.145M glucose-C(14) is dependent on, but not linearly related to, the amount of ethanolamine phosphoglyceride in the mixture. The leakage of swelling solution out of such myelinoid lipid-water dispersions was, however, linearly related to the proportion of ethanolamine phosphoglyceride.

Chemical Phenomena↗