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Biomedical subjects

P G Howell

Publications and source records attributed to P G Howell.

At least 19 recordsLinked to original sources
PubMed2003

A group-specific, indirect sandwich ELISA for the detection of equine encephalosis virus antigen.

A polyclonal antibody-based, group-specific, indirect, sandwich ELISA (S-ELISA) for the detection of equine encephalosis virus (EEV) antigen was developed. Purified EEV particles were titrated in the S-ELISA and the limit of detection was determined to be approximately 9.0 ng of antigen/ml (0.45 ng/well). Positive S-ELISA reactions were recorded with seven serologically distinct EEV serotypes. No cross-reactions were recorded with other arboviruses including African horse sickness virus (AHSV) serotypes 1-9, bluetongue serotypes 1-24, epizootic haemorrhagic disease serotypes 1-8 and isolate 318, and selected isolates of Palyam, Eubenangee, Corriparta, Warrego, Akabane and bovine ephemeral fever viruses. The assay proved to be sensitive and specific for the rapid detection of EEV in cell cultures and in homogenated suckling mouse brain (MB). The data generated in this study suggest that the ELISA will be valuable for epidemiological studies of EE and will assist in making a reliable differential diagnosis between EEV and AHSV infections.

Animals↗
PubMed2003

Variation in the NS3 gene and protein in South African isolates of bluetongue and equine encephalosis viruses.

Bluetongue virus (BTV) and equine encephalosis virus (EEV) are agriculturally important orbiviruses transmitted by biting midges of the genus Culicoides. The smallest viral genome segment, S10, encodes two small nonstructural proteins, NS3 and NS3A, which mediate the release of virus particles from infected cells and may subsequently influence the natural dispersion of these viruses. The NS3 gene and protein sequences of South African isolates of these viruses were determined, analysed and compared with cognate orbivirus genes from around the world. The South African BTV NS3 genes were found to have the highest level of sequence variation for BTV (20 %), while the highest level of protein variation of BTV NS3 (10 %) was found between South African and Asian BTV isolates. The inferred NS3 gene phylogeny of the South African BTV isolates grouped them with BTV isolates from the United States, while the Asian BTV isolates grouped into a separate lineage. The level of variation found in the NS3 gene and protein of EEV was higher than that found for BTV and reached 25 and 17 % on the nucleotide and amino acid levels, respectively. The EEV isolates formed a lineage independent from that of the other orbiviruses. This lineage segregated further into two clusters that corresponded to the northern and southern regions of South Africa. The geographical distribution of these isolates may be related to the distribution of the Culicoides subspecies that transmit them.

African Horse Sickness Virus↗
PubMed2003

Lateral transmission of equine arteritis virus among Lipizzaner stallions in South Africa.

REASONS FOR PERFORMING STUDY: A serological study conducted in 1995 revealed that 7 stallions at the Lipizzaner Centre, Gauteng, South Africa, were seropositive for antibody to equine arteritis virus (EAV). A Lipizzaner stallion imported into South Africa from Yugoslavia in 1981 had previously (1988) been confirmed to be an EAV carrier. Despite being placed under life-long breeding quarantine, EAV had been transmitted between stallions at the Lipizzaner Centre. OBJECTIVES: To investigate the phylogenetic relationships between the strain of EAV shed in the semen of the original carrier stallion and strains recovered from the semen of 5 other stallions; and to investigate the means whereby lateral transmission of EAV occurred among 7 in-contact, nonbreeding stallions at the Centre. METHODS: EAV was isolated from semen collected from the seropositive stallions using RK-13 cells. Viral RNA was reverse transcribed and amplified by polymerase chain reaction using ORF 5-specific primers, subjected to sequence and phylogenetic analysis. RESULTS: Phylogenetic analysis of strains of EAV recovered from the semen of 6 persistently infected stallions confirmed that all viruses were closely related and probably derived from a common ancestor, i.e. the stallion imported from Yugoslavia. Lateral transmission subsequently occurred among 7 in-contact, nonbreeding stallions at the Centre. It is speculated that these stallions may have been exposed to virus from bedding or fomites contaminated with semen. CONCLUSIONS: These data confirm that lateral transmission of EAV can occur from shedding stallions to susceptible, in-contact horses, including other stallions, which may become persistently infected with the virus. POTENTIAL RELEVANCE: The findings are consistent with lateral spread of a single, unique strain of EAV among a group; and suggest that transmission of EAV may be initiated by infection of one or more stallions with virus on bedding or other fomites contaminated with EAV- infected semen.

Animals↗
PubMed2003

West Nile virus infection of Thoroughbred horses in South Africa (2000-2001).

REASONS FOR PERFORMING STUDY: West Nile virus (WNV) infection is endemic in southern Africa. With the recent emergence of WNV infection of horses in Europe and the USA the present study was performed to estimate the risk of seroconversion to WNV in a cohort of 488 young Thoroughbred (TB) horses. OBJECTIVES: To estimate the risk of seroconversion to WNV among a cohort of South African TB yearlings sold at the 2001 National Yearling Sales (NYS) and to determine whether the risk varied geographically. Two horses were also infected with a recent South African isolate of WNV to evaluate its virulence in horses. METHODS: Serum samples were collected from the cohort of 488 TB yearlings at the 2001 NYS. Serum samples that were collected from the same horses at the time that they were identified were sourced from our serum bank. Sera from 243 of the dams that were collected at the time that the foals were identified were also sourced from our serum bank. These sera were subjected to serum neutralisation (SN) tests for antibody to WNV. RESULTS: Approximately 11% of yearlings seroconverted to WNV on paired serum samples collected from each animal approximately 12 months apart. Studfarms with WNV-seropositive yearlings were widely distributed throughout South Africa and SN tests on sera from their dams indicated that exposure to WNV was even more prevalent (75%) in this population. Neurological disease was not described in any of the horses included in this study and 2 horses inoculated with a recent lineage 2 South African isolate of WNV showed no clinical signs of disease after infection and virus was not detected in their blood. CONCLUSIONS: Infection of horses with WNV is common in South Africa, but infection is not associated with neurological disease. POTENTIAL RELEVANCE: In contrast to recent reports from Europe, North Africa, Asia and North America, the results of our field and experimental studies indicated that exposure of horses to the endemic southern African strains of WNV was not associated with neurological disease.

Animals↗
PubMed2002

A comparison of the vector competence of the biting midges, Culicoides (Avaritia) bolitinos and C. (A.) imicola, for the Bryanston serotype of equine encephalosis virus.

Equine encephalosis virus (EEV) is widespread and prevalent in southern Africa. In this study, the oral susceptibility of Culicoides (Avaritia) imicola Kieffer (Diptera: Ceratopogonidae) to EEV was confirmed. In addition, C. (A.) bolitinos Meiswinkel, collected in the high-lying eastern Free State, South Africa, was systemically infected with the Bryanston serotype of EEV after feeding through a membrane on artificially infected equine blood containing 4.7 log10 PFU/mL of EEV. The mean infectivity of Bryanston virus in C. bolitinos increased from 1.2 log10 PFU/midge, in midges assayed for virus immediately after feeding on the blood-virus mixture, to 3.1 log10 PFU/midge in midges assayed after 10 days' incubation at 23.5 degrees C. Elevated virus infectivity titres, found in individual infected C. bolitinos, suggested that this Culicoides species is a vector of EEV. This bovine dung-breeding Culicoides species may play an important role in transmitting EEV in the cooler parts of southern Africa, where it can be the most abundant Culicoides species collected near livestock. In the present study the prevalence of infection obtained for C. bolitinos (2.2%) with the Bryanston serotype of EEV was significantly lower than that of C. imicola (18.4%). After incubation, the Bryanston serotype of EEV was also isolated from one of 110 C. onderstepoortensis Fiedler assayed. However, the virus titre in this midge was 1.2 log10 PFU/midge, which is not different from the titre that would be expected immediately after feeding on the blood-virus mixture. Culicoides species that survived the incubation period and that were negative for the presence of Bryanston virus were C. magnus Colaço (96), C. bedfordi Ingram & Macfie (95) and C. pycnostictus Ingram & Macfie (45).

African Horse Sickness↗
PubMed2002

The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa.

Selected isolates of equine encephalosis virus were shown to have comparable viral protein profiles and to represent seven distinct serotypes, based on cross-neutralization tests. Serotype-specific virus-neutralizing antibody in serum samples from horses confirmed the widespread occurrence of infection. The distribution and prevalence of individual serotypes however, varied considerably. Localised foci with an increased seasonal seroconversion in groups of horses to a specific serotype and the detection of an ongoing low level of infection from other serotypes within the population, confirmed the independent persistence of the viruses in a maintenance cycle. The identification of donors with antibody resulting from infection with multiple serotypes indicated a low level of cross-protection in horses to natural reinfection.

Animals↗
PubMed2001

A model of temperature transients in dental implants.

Dental implants provide a continuous interface between the oral environment and the deep core structures of the jaws. Implants and trans-mucosal superstructures are primarily metal and heat conduction occurs readily. A hypothetical heat conduction model is investigated to determine the ranges of temperature gradients that might occur in implants. This model showed that a 60 degrees C heat source will cause a heat front of > or = 47 degrees C to advance > 3 mm down an implant within one second. Oral temperature transients may be a factor in implant pathology.

Dental Implants↗
PubMed2000

The size of occlusal rest seats prepared for removable partial dentures.

OBJECTIVE: The aim of this study was to test whether rest seats cut by a group of general dental practitioners for a removable partial denture differed in size and shape from those prepared by either a group of postgraduate students or their academic teachers. METHOD: The occlusal surfaces of a number of plastic teeth were scanned by a laser profilometer. Each tooth was then placed in a set of articulated phantom head dental arches. 30 dental practitioners, 16 postgraduates and 11 dental academics were asked to cut a rest seat preparation in the mesial marginal ridge suitable for the construction of a removable chromium cobalt partial denture. The tooth was removed from the models, rescanned, and this data converted to grey level images for measurement of the width, length and area of each rest seat. Depth was calculated as the difference between the pre and post preparation scanned profiles. RESULTS: There was a wide variation in the size of the individual rest seat preparations. There was no significant difference between the measured parameters from the images of the preparations made by academic staff and postgraduate students. The two sets of data were therefore combined. The length, width and area of the rest seats prepared by the staff and postgraduate group were significantly greater than those cut by the dental practitioner group. However, there was no significant difference in the depths measured. The outline form of the rests prepared by the dental practitioners was often round with sharply defined margins contrasting with the smooth triangular preparation the staff and postgraduates prepared. CONCLUSION: A 'refresher' in tooth modification for GDPs designing partial dentures would improve the long term success of the prosthesis.

Clinical Competence↗
PubMed2000

A biometric guide to prosthetic tooth positioning investigated in subjects with Class II division 2 incisor relationships.

A biometric guide for positioning of the maxillary central incisors in complete dentures is the incisor-incisive papilla distance. This distance was compared in dentate subject with either Class I or Class II/2 incisor relations. Maxillary casts were orientated parallel to the occlusal plane on an xy table, connected to a movable vertical arm. The distance from the posterior edge of the papilla to the labial incisor surface was measured. Results showed a mean difference of 2 mm between the groups, significant at the P < or = 0.001 level, however this distance would not appear to invalidate the conventional biometric guideline of 10 mm.

Adult↗
PubMed1999

Vector competence of selected South African Culicoides species for the Bryanston serotype of equine encephalosis virus.

Equine encephalosis virus (EEV) was recognized and described in the Republic of South Africa in 1967 and subsequent serological studies have shown this orbivirus to be both widespread and prevalent in southern Africa. In the present study it was shown that wild-caught Culicoides (Avaritia) imicola Kieffer (Diptera: Ceratopogonidae) can become infected with and permit the replication of the Bryanston serotype of EEV following membrane-feeding on infective blood containing 5.0 log10 plaque-forming-units (PFU)/ml. The mean prevalence of Bryanston virus infection in C. imicola after 10 days extrinsic incubation at 23.5 degrees C was 22.3% (23/103). The mean infectivity of Bryanston virus in the infected C. imicola increased from 1.3 log10 PFU/midge, in insects assayed immediately after feeding on the blood-virus mixture, to 2.6 log10 PFU/midge in insects assayed after incubation. The virus concentration in individual C. imicola infected with the Bryanston serotype of EEV ranged from 0.7 to 3.6 log10 PFU/midge. Bryanston virus titres higher than 2.5 log10 TCID50, found in individual C. imicola, suggest that this species may be able to transmit this virus to susceptible hosts. Prevalence of virus infection in C. imicola was determined by PFU and microtitration assays on both BHK and Vero cells and confirmation of the Bryanston serotype of EEV was determined by plaque inhibition. No virus replication could be demonstrated in 102 C. nivosus tested after the incubation period, suggesting that not all Culicoides species are equally susceptible to Bryanston virus infection. Other Culicoides species that survived the incubation period and that were negative for the presence of Bryanston virus were C. pycnostictus (42), C. leucostictus (7), C. magnus (2), C. bolitinos (1) and C. bedfordi (1).

Africa, Southern↗
PubMed1998

Monte Carlo simulation of electron backscattering from compounds with low mean atomic number.

This paper reports a Monte Carlo simulation where a single atom scattering model is adopted. The element taking part in each electron-atom interaction is selected on the basis of its contribution either to the total elastic cross section or to the electron's mean free path. Both Rutherford and Mott scattering are considered, with the continuous slowing down process of Bethe used to calculate the energy loss to the system. The backscattered electron coefficients show good agreement with experimental results from a large group of low atomic number materials when using a model which selects the scattering atom by its contribution to the whole compound calculated from its atomic fraction of the total elastic cross-section.

Computer Simulation↗
PubMed1995

A comparative microbiological study of clinically healthy eyes and those affected by ophthalmia in cattle and the association of noctuid eye-frequenting moths.

The eyes of clinically healthy Simmentaler cattle and those affected by ophthalmia were sampled once a month over a continuous period of 12 months for bacterial, mycoplasmal and ureaplasmal infections. In total 478 eyes, representing from a clinical viewpoint 414 healthy and 64 affected eyes, were swabbed. Bacteria were isolated from 201 (48.6%) healthy eyes and 56 (87.5%) affected eyes. No bacteria were isolated from the remaining eyes. Eleven genera of bacteria were isolated from healthy eyes and 8 genera from affected eyes. The majority of isolates were classified in the genera Moraxella, Neisseria and Staphylococcus. Mycoplasmas were isolated from 247 (50.7%) healthy eyes and 27 (42.2%) affected eyes. No mycoplasmas were isolated from the remaining eyes. Ureaplasmas were not isolated from any animal. Eye-frequenting moths were collected on 3 occasions during the investigation and bacterial and mycoplasmal isolation techniques were performed on a total of 21 moths. Twelve different genera of bacteria, mostly Nocardia, Corynebacterium, Staphylococcus, Moraxella, and mycoplasmas were isolated from various eye-frequenting moths. Scanning electron microscopical studies of the proboscis of the moths showed it to contain various sensillae and short triangular denticles that could possibly cause damage to the mucous membranes of the eyes and predispose to ophthalmia in cattle.

Animals↗
PubMed1993

The recording and analysis of EMG and jaw tracking. II. Reproducibility of jaw tracking.

In 10 male and 10 female adults jaw movements and muscle activity were monitored during chewing. Subjects had a Class I occlusion and fluent unrestricted jaw movements; non exhibited signs or symptoms of craniomandibular disorders. Recordings were made on two occasions separated by 2 weeks. Within each recording session the subjects carried out a number of tasks under direction, including eating nuts and gum. The jaw movements were monitored with a Sirognathograph which had been interfaced to a computer for data logging and analysis. A computer program identified each chewing stroke and after scrutiny a data base was constructed of measurements from a subset of 4447 suitable strokes. Mean values of 10 parameters selected to describe jaw movements during chewing were entered into an ANOVA. This indicated that the major contribution to variance was due to the subject from whom the data was recorded. This intersubject variability is well known, but can its separation from the other contributions to the variation within the data be assessed? To provide an estimate of the spread of data the variance for each parameter was calculated, transformed and these values entered into an analysis of variance. All subject differed significantly in their chewing movements from one another. But 15 of the 20 subjects showed a greater degree of consistency than the other five: they exhibited good inter- and intra-recording reproducibility. The food being eaten made a major contribution to the variability. The timing of the chewing strokes was also more consistent than the dimensions of the chewing envelope.

Adult↗
PubMed1992

The recording and analysis of EMG and jaw tracking. I. The recording procedure.

A computer-based system is described which records and analyses electromyographic (EMG) signals and tracking data for mandibular movements during function. EMG signals were obtained bilaterally from six muscles and a Sirognathograph monitored the position of the lower incisor teeth in three dimensions. Directed jaw movement tasks by subjects were monitored. These data were continuously sampled at a rate of 1 KHz per channel over an operator controlled period. Analysis of the data was performed off-line following the experiment. Individual chewing cycles associated with masticatory function were identified and the timing and dimensions of each chewing envelope established. Corresponding EMG values were also determined and displays of EMG and jaw movement were plotted on a common time base. An average chewing cycle and correlated EMG activity could also be displayed on a high resolution monitor and plotted for a hard copy to enable a comparison to be made between subjects with and without jaw dysfunction.

Analysis of Variance↗
PubMed1989

Some husbandry factors influencing weaning stresses in piglets.

The post-weaning diarrhoea syndrome (PWDS) in piglets is multi-factorial in origin. Numerous managemental, environmental, housing, nutritional, immunological and physiological factors are discussed, being primary factors in the cause of the PWDS. Infectious agents especially Escherichia coli are often incriminated as causes of PWDS but are more likely to be opportunistic elements due to faulty management of stressed animals.

Animal Husbandry↗