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Biomedical subjects

P G Storozhuk

Publications and source records attributed to P G Storozhuk.

At least 19 recordsLinked to original sources
PubMed2000

[Measurements of salivary lysozyme activity].

A new method for measuring salivary lysozyme is proposed, which is based on lysozyme capacity to lyze glycosaminoglycanes and proteoglycanes in plasma membranes of Micrococcus lysodeikticus. Specific activity of the enzyme is identified from the amount of protein in glycosaminoglycanes and proteoglycanes. The quantity of glycosaminoglycanes and proteoglycanes containing 0.001 mg protein/ml/min is suggested to be takes as a unit of lysozyme.

Clinical Laboratory Techniques↗
PubMed1990

[Determination of the erythrocyte count using a microcolorimeter].

Red cell count is estimated with the use of a MKMF-1 microcolorimeter. 10 ml of Gower's solution are used; 0.02 ml of blood are added to this solution. Photometry of red cell suspension is carried out in a cuvette with a 0.5 cm optic route at lambda 610 nm. Microampermeter data are read from the optic density D scale. The D value is substituted into the formula: red cell count (RC) = K.D.10(6), where K coefficient is found experimentally by measuring the D and by estimating red cell count in microscopy, and is equal to 33.3. This formula permits estimating red cell count in 1 microliter of blood.

Colorimetry↗
PubMed1990

[Substantiation of the use of pepsidil in secretory insufficiency of the digestive glands caused by antitubercular drugs].

The experiments on rats were undertaken to study the action of pepsidil to make up for the biosynthesis deficiency of digestive enzymes in the stomach and pancreas caused by antituberculous drugs (methazide + PAS, isoniazid + PAS, isoniazid + rifampicin + ethambutol). It was proved that pepsidil reduced an inhibiting action of antituberculous drugs on the activity of gastroenteric tract proteinases. The given condition should be taken into account when antituberculous treatment is conducted.

Animals↗
PubMed1990

[Value of the study of various erythrocyte enzymes in determining the nature of cerebral stroke in the acute period].

Activities of catalase, superoxide dismutase, glyceraldehyde phosphatedehydrogenase and glucose-6-phosphate dehydrogenase implicated in the process of hemoglobin oxygenation were studied and compared in 40 patients with ischemic and in 30 patients with hemorrhagic brain strokes in the most acute disease period (days 1-2). A well-defined relationship was revealed between the brain stroke pattern and enzymatic activity which is likely to be determined by varying degree of body adaptation under hypoxia developing in ischemic and hemorrhagic brain strokes. It is assumed that the clinico-enzymatic correlations discovered might be employed as additional criteria in the differential diagnosis of the brain stroke pattern.

Acute Disease↗
PubMed1989

[Effect of antitubercular preparations on enzyme activity of erythrocytes].

It was established experimentally that antituberculous agents (isoniazide, rifampicin, methazid, ethambutol, PAS) and their combination (isoniazide + rifampicin + PAS; isoniazide + PAS; methazid + PAS) inhibit erythrocytic glucoso-6-phosphate-dehydrogenase, glycerophosphate-dehydrogenase. This should be considered in instituting individual antituberculous therapy.

Animals↗
PubMed1981

[Polypeptides isolated from the gastric mucosa and their effect on pepsin biosynthesis by gastric glands].

It was found in experiments on 90 rats that polypeptides A, B and C (with a molecular mass of 2200-2800) isolated from the gastric mucosa have the ability to stimulate pepsin biosynthesis by the gastric glands. Application of the polypeptides did not result in the concurrent rise of proteolytic and milk-curdling activity. Pepsin and trypsin promoted the release from polypeptide B of low-molecular weight peptides which also stimulated pepsin biosynthesis.

Animals↗
PubMed1981

[Effect of diacarb on the carbonic anhydrase activity in the blood and gastric mucosa and pepsinogen concentration in the gastric mucosa of the rat].

It was established that the activity of blood and gastric mucosa carboanhydrase increased after the introduction of food irritant (milk) into the stomach, as well as after the subcutaneous injection of histamine. This was accompanied by the increase of pepsinogen content in the gastric mucosa. When introduced into the stomach before the food irritant or histamine, acetazolamide inhibited blood and gastric mucosa carboanhydrase and reduced the content of pepsinogen in the gastric mucosa. Oral administration of acetazolamide for 5 days resulted in a more remarkable inhibition of blood and gastric mucosa carboanhydrase and in a drastically reduced content of pepsinogen in the gastric mucosa. The rate of pepsinogen biosynthesis by the gastric mucosa seems to depend on the activity of carboanhydrase in blood and in the gastric mucosa.

Acetazolamide↗
PubMedDate not supplied

[Effect of certain antibiotics of tetracycline series on the level of blood sugar and the role of insulin in the mechanism of its regulation].

Experiments on male rats showed enterally administered tetracycline and chlortetracycline to promote an elevation of the blood sugar level. These antibiotics administered once together with glucose retarded the normalization of the blood sugar concentration. After 7-day administration of tetracycline and chlortetracycline glucose load caused a stable hyperglycemia. Special experiments with depancreatization and insulin injection to the animals and also the intravenous injection of glucose and the antibiotic demonstrated that the changes in the blood sugar concentration under the effect of tetracycline were associated both with its inhibitory action on the absorbing function of the intestine and with the retarded glucose utilization in the tissues. Insulin injected intramuscularly eliminated the hyperglycemic effect caused by the antibiotics.

Animals↗
PubMedDate not supplied

[Enzyme-forming function of digestive glands after administration of polypeptide C, copepsidyl and panintestine].

Polypeptide C (molecular weight 2640 Dalton) extracted from artificial gastric juice), copepsidyl (containing high dosage of pepsin) and panintestine (polyenzyme drug) are studied for their effect on the activity of digestive enzymes of glandular gastric element pancreas and small intestine of rats. It is established that all mentioned drugs stimulate enzymogenesis in the analyzed organs. The activity of pepsin increases in homogenates of gastric mucosa, the activity of trypsin, total proteinases, carboxypeptidase and amylase grows in pancreas homogenates, and that of leucineaminopeptidase--in small intestine.

Animals↗
PubMedDate not supplied

[Changes in glyceraldehydephosphate dehydrogenase, glucose-6-phosphate dehydrogenase, superoxide dismutase, and catalase activity in erythrocytes depending upon the rate of glucose utilization].

Content of glucose as well as activities of glyceraldehyde phosphate dehydrogenase (alpha-GPD), glucose-6-phosphate dehydrogenase (G6PD), superoxide dismutase (SOD) and catalase were studied in whole blood, in intact and hemolyzed erythrocytes of 57 volunteers within 120 min after sucrose loading. Direct correlation was found between the activity of the enzymes studied and level of glucose as well as the rate of its utilization in erythrocytes. These data suggest that reduced NAD- and NADP-containing oxidoreductases alpha-GPD and G6PD are donors of H+ used in biosynthesis of H2O2 catalyzed by SOD. Intact and hemolyzed erythrocytes are involved in destruction of H2O2 accompanied by liberation of O2, which reacted with Hb more readily as compared with atmospheric oxygen.

Adult↗