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Biomedical subjects

P Geiger

Publications and source records attributed to P Geiger.

29 records · Page 2Linked to original sources
PubMed1991

[Experiences with autologous donation and transfusion equipment].

For autologous plasma predeposit there are commonly two types of cell-separators in use: 1. Discontinuous centrifugation (Plasma Collection System PCS, Haemonetics Company). 2. Discontinuous membrane filtration (Plasmapur-Monitor, Organon Teknika Company). Normally donation volume is 900 ml. The rate of undesired secondary effects does not exceed those numbers known of regular homologous donors. Shed wound blood processing by a wash-centrifuge cell separator (Type Cell-Saver, Haemonetics Company) stands for optimal quality of the refusable red blood cells. Depending on the type of operation and the accuracy of wound blood suction, up to 75% of the red blood cells lost may be harvested. In order to achieve more widespread mechanical autotransfusion, one issue is to lower the rather high costs of the disposables by simplifying the too highly sophisticated systems. From clinical experience with orthopedic patients, at least practical advice is given on how to use the single autologous transfusion methods in a comprehensive strategy.

Blood Component Removal↗
PubMed1991

[Kinetics of bacterial wash-out in the Haemonetics Cell Saver III].

Autologous, outdated blood units were inoculated with four types of bacteria--Pseudomonas aeruginosa, Escherichia coli, Streptococcus faecalis, Staphylococcus aureus--in two concentrations (10e4, 10e6) to test the effect of the washing procedure (Cell Saver III Haemonetics) on the elimination of bacteria. The elimination rate ranged from 17% (Streptococcus faec. 10e4) to 96% (Pseudomonas aer. 10e6). These results confirm the routine use of wash centrifuge systems (type Cell Saver) to process shed wound blood as well as drainage blood for autologous transfusions.

Blood↗
PubMed1990

[Differentiated blood replacement therapy in endoprosthesis surgery of the hip joint].

In a prospective clinical study with 37 patients (41-84 years) undergoing a first implantation of hip joint endoprothesis, a total blood loss of two liters in average was registrated including the first 24 h postoperative wound drainage blood. Sufficient volume replacement was achieved without any homologous blood components by consequently using a comprehensive concept of autologous transfusion including acute preoperative hemodilution, intra and postoperative blood salvage and preoperative plasma predeposit (ATU = Autologous Transfusion-Concept of Ulm). Plasma and blood substitution was managed mainly by polygelin and autologous fresh frozen plasma completed by autologous warm blood and processed autologous washed packed red cells. Through this any organization troubles or disadvantages of whole blood predeposit may be avoided. To increase the net gain of washed packed red cells time limited use of blood salvage disposables should be extended.

Adult↗
PubMed1990

[4 years experience with the Ulm autologous transfusion concept].

After a 4-year period in clinical practice the autologous transfusion concept Ulm (ATU) has proved its value. The effort and expense involved are entirely justified by obvious medical advantages. The patients' active involvement in the therapeutic procedure is a remarkable aspect of positive motivation. Furthermore, the medical staff is positively motivated, too, in spite of the obvious additional load to their daily routine work.

Blood Transfusion, Autologous↗
PubMed1988

The effect of pretreatment with pentobarbital on the extent of [14C] incorporation from [U-14C]glucose into various rat brain glycolytic intermediates: relevance to regulation at hexokinase and phosphofructokinase.

In the present investigation we monitored the incorporation of [14C] from [U-14C]glucose into various rat brain glycolytic intermediates of conscious and pentobarbital-anesthetized animals. Labeled glucose was delivered to brain by single bolus intracarotid injection and brain tissue was subsequently prepared at 15, 30, and 45 sec by freeze-blowing. Glycolytic intermediates were then separated by column chromatography. Our results showed a gradual decrease with time of 14C-labeled glucose which gave a calculated rate for glucose metabolism of 0.86 mumol/min/g and 0.56 mumol/min/g in conscious and anesthetized animals, respectively. Compared to the results obtained using conscious animals the administration of pentobarbital not only resulted in a significant attenuation of the rate of glucose metabolism but also caused a similar reduction in the amount of 14C incorporated into several glycolytic intermediates. These intermediates included: glucose 6-phosphate, fructose 6-phosphate, fructose 1,6 diphosphate, dihydroxyacetone phosphate and post glycolytic compounds. In addition, pretreatment with pentobarbital resulted in a 75% increase in the endogenous concentration of glucose, 10% increase in glucose 6-phosphate, no change in fructose 6-phosphate and 42% decrease in lactate compared to levels in brains obtained from conscious animals. These results are discussed in relation to control of glycolysis through coupled regulation at hexokinase-phosphofructokinase.

Animals↗
PubMed1982

Impaired energy metabolism in rat myocardium during phosphate depletion.

The effects of phosphate depletion (PD) of 4, 8, and 12 wk duration on myocardial energy metabolism were studied in rats fed a phosphate-deficient diet and compared with rats pair-fed a normal phosphate diet. Myocardial biopsies were examined for high-energy phosphate bonds. The results show that PD causes a significant reduction in myocardial concentration of inorganic phosphorus at 4 wk of PD and creatine phosphate at 8 wk of PD, while adenine nucleotides were significantly reduced only after 12 wk of PD. The changes in cellular inorganic phosphorus and creatine phosphate displayed a significant correlation with serum phosphorus levels. Mitochondrial respiration was impaired early in PD. Total cellular, mitochondrial, and myofibrillar creatine kinase activities were significantly reduced at 4 wk of PD and fell further at 8 and 12 wk. These data show that chronic PD is associated with reduced mitochondrial capacity to produce ATP, impaired transport via the creatine phosphate shuttle, and reduced myofibrillar ability to utilize ATP. These abnormalities indicate that all steps of myocardial energetics are impaired in PD and provide the molecular basis for the altered myocardial function seen in PD.

Animals↗
PubMedDate not supplied

[Combined caudal and general anesthesia in clubfoot surgery].

In a retrospective study we analysed the effect of caudal epidural anesthesia combined with general anesthesia on requirement for intraoperative narcotics and postoperative analgesics in children undergoing surgical release of congenital clubfoot. In 20 children (mean age at day of surgery 4,7 months) general anesthesia was supplemented by caudal epidural anesthesia, controls (20 children, mean age 4,3 months) received general anesthesia only. Compared to controls, the amount of narcotics was significantly reduced in the caudal epidural group (1,1 Vol% compared to 1,6 Vol% Isofluran). As well the use of analgesics in the postoperative phase was decreased from 112,5 mg to 56,3 mg Paracetamol. The children in the caudal epidural group recovered from anesthesia more comfortably. No complications occurred in either group. Our results show, that general anesthesia combined with caudal epidural anesthesia is more suitable for surgical release of congenital clubfoot than the exclusive use of general anesthesia.

Analgesics↗