Biomedical subjects

P Gilbertson

Publications and source records attributed to P Gilbertson.

3 recordsLinked to original sources

PubMed2001

The prenatal visit.

In their role as advocates for children and families, pediatricians are in an excellent position to support and guide parents during the prenatal period. Prenatal visits allow the pediatrician to gather basic information from parents, provide information and advice to them, and identify high-risk situations in which parents may need to be referred to appropriate resources for help. In addition, prenatal visits are the first step in establishing a relationship between the pediatrician and parents and help parents develop parenting skills. The prenatal visit may take several possible forms depending on the experience and preferences of the parents, competence and availability of the pediatrician, and provisions of the health care plan.

Adult↗

PubMed1987

Cellular requirements for mixed leucocyte reactivity in the cyclostome, Eptatretus stoutii.

Unfractionated peripheral blood leucocytes from allogeneic hagfish proliferated vigorously in one-way mixed leucocyte reaction (MLR) at 20-22 degrees. Maximum responsiveness was observed after 5 days of co-culture of responder and irradiated stimulator cells. Separation of leucocytes on the basis of laser scatter parameters yielded a small leucocyte population which responded but did not stimulate in MLR and a large leucocyte population capable of stimulating but not responding. Adherent cell depletion of the total leucocyte population did not affect the ability to respond in MLR but did ablate the stimulatory capacity of hagfish leucocytes. Together with previous phenotypic studies, these results confirm the presence of lymphocyte and macrophage/accessory cell populations in hagfish peripheral leucocytes.

Animals↗

PubMed1986

Evolutionary development of lymphocyte heterogeneity: leucocyte subpopulations in the Pacific hagfish.

Flow cytometric analysis of forward angle versus 90 degree scatter patterns of hagfish peripheral blood revealed two distinct leucocyte populations with size characteristics analogous to mammalian monocytes/granulocytes (hagfish large leucocytes) and small lymphocytes (hagfish small leucocytes). A cell population enhanced for the small leucocytes was obtained by density gradient centrifugation. Over 70% of the small leucocyte population consistently stained with a rabbit antiserum directed against polypeptide determinants on hagfish immunoglobulin, while staining of the large cell population was greatly reduced (less than 10%). A panel of monoclonal antibodies raised against a crude hagfish leucocyte preparation distinguished the two cell populations and revealed the existence of subpopulations of both small and large leucocytes.

Animals↗