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Biomedical subjects

P Grigolato

Publications and source records attributed to P Grigolato.

14 recordsLinked to original sources
PubMed1992

Suppurative granulomatous lymphadenitis. Immunohistochemical evidence for a B-cell-associated granuloma.

The cellular composition of suppurative granulomas was investigated by the application of monoclonal and polyclonal antibodies to paraffin sections and compared with nonsuppurative, hypersensitivity-type granulomas. Macrophages, T cells, and dendritic cells showed a similar distribution in both types of granulomas. In addition to the presence of granulocytes, a major difference between suppurative granulomas and hypersensitive-type granulomas concerned their relationship with B lymphocytes. Hypersensitive-type granulomas were surrounded by small mantle B cells, but they did not contain any B lymphocytes. In contrast, variable numbers of B cells were found either at the periphery or in the center of suppurative granulomas. In view of their morphology (medium size, pale cytoplasm, irregular nuclear shape) and phenotype (L26 +/LN1 -/MB2 +/MT2 +) these B lymphocytes closely resembled monocytoid B cells. The monocytoid B cells might have a role in the recruitment of polymorphonuclear granulocytes and in the development of the necrosis, which occur within suppurative granulomas.

Acid Phosphatase↗
PubMed1991

Static cytometry of DNA in breast lump with reference to grading.

Fresh smears of 400 consecutive cases of breast lumps stained with Feulgen stain were measured with densitometric technique in static cytometry. A correlation was observed between DNA content expressed by the DNA index and histologic grading of malignant lesions; relatively high values were observed in the group of "hyperplasias", which were similar to G1. Values of DNA content in different categories: benign, hyperplastic (nodular and sclerosing adenosis, epitheliosis . . .) and all malignant types variously graded in G1, G2 and G3, show broad areas of overlapping, suggesting that the parameter is not always discriminating between benign, hyperplastic and malignant, but it can identify different amounts of DNA (high and low degrees) within a homogeneous category. Correlation between number of hypertetraploid nuclei (4c) and histologic grading was also evident. Therefore, fresh smears, in relation to DNA content and to the percentage of hypertetraploid elements allow a better definition of "high and low degree" lesions in a short time with possible prognostic involvement as regards tumoral progression.

Breast Neoplasms↗
PubMed1989

Histological features of uteroplacental vessels in normal and hypertensive patients in relation to birthweight.

Placental bed biopsies were obtained during caesarean section from normal, pre-eclamptic and hypertensive pregnancies. Of the 14 biopsies from normal pregnancies 13 showed normal vascular physiological changes in the decidua and in the myometrium. Biopsies from 24 pregnancies complicated by pre-eclampsia showed acute atherosis in 18 and physiological changes limited to the decidua in six; none had normal physiological changes. Biopsies from five hypertensive patients showed all three types of histological changes. The mean birthweight centile was lower in the group with atherosis than in the group with limited and the group with normal physiological changes.

Birth Weight↗
PubMed1987

Blue nevus of the endocervix. A study of five cases.

The pathologic and immunohistochemical features of 5 cases of blue nevus of the endocervix are presented: 4 of them were studied ultrastructurally. The lesions were incidentaly discovered at microscopic examination and showed pigmented dendritic cells in the cervical stroma. Immunocytochemical examination showed all cases to be positive for S-100 protein. Ultrastructurally they contained melanosomes, were surrounded by a basement membrane, and displayed occasional desmosome-like devices. Histogenesis is discussed, and support for a schwannian origin is presented.

Cystadenoma↗
PubMed1987

Ultrastructural renal findings in allografted kidneys of patients treated with ciclosporin A.

22 renal biopsies of 22 patients with 6-month-old transplants have been examined at the light- and electron-microscopic level to demonstrate the toxic effects induced by ciclosporin A (CS). These patients presented stable renal function and were exempt from acute rejection symptoms or nephrotoxicity. Interstitial fibrosis, tubular atrophy and interstitial lymphocytic infiltration were the lesions most often observed by light microscopy. In a single case an arteriolar lesion suggestive of arteriolopathy due to Cs was seen. Ultrastructural observations of renal tubular cells showed a tendency of rough and smooth endoplasmic reticulum to microdilate and microvacuolize. In 4 out of 22 cases there were mitochondrial alterations with giant mitochondria. In only 1 case, clusters of cilia were seen. Clinical histological correlations show that interstitial fibrosis is directly proportional to CS blood level. CS higher blood levels suggest a higher risk of nephrotoxicity.

Basement Membrane↗
PubMed1977

Experience of treatment in the Amanita phalloides poisoning (clinical and experimental observations).

Intensive care was applied in 13 patients with acute liver insufficiency and different degrees of metabolic encephalopathy due to the ingestion of Amanita phalloides. Twelve of them were subjected to hepatic assist with E.T., in three cases this measure was repeated. Ten recoveries were obtained, which denote, on the basis of the necrosis enzymes and the hepatic histo-pathological aspects from bioptic samplings, the use of the E.T. method, when applied within the first 72 hours. In pigs poisoned with Amanita phalloides, the clinico-biochemical pattern and the histopathological findings are comparable to the observations in man. Electron microscopy demonstrates peculiar lesion in the cytoplasm. The anatomo-pathological findings in the three dead patients show a liver necrosis over the 90% and the presence of cerebral edema.

Adolescent↗
PubMedDate not supplied

Growth fraction of colorectal carcinoma (Ki67): a comparative study.

We studied the growth fraction of 55 resected colorectal adenocarcinomas by means of a three-step immunoperoxidase technique (avidin-biotin-peroxidase) using the monoclonal antibody Ki67 directed against a cell proliferation-associated nuclear antigen. The percentage of Ki67-positive cells was evaluated independently by two observers, and a Ki67 score was obtained for each case. No correlation was observed between Ki67 staining and patient's age and sex, tumor size and localization or grading and staging according to Dukes' method (modified by Astler-Coller and Turnbull). The growth fraction showed extreme heterogeneity in the cases examined, within each grade of differentiation.

Adult↗
PubMedDate not supplied

[DNA index of colorectal carcinoma and follow-up].

In relation to DNA index we have studied 18 cases of differently evoluted carcinoma of the colon, some incoherently (8) other in agreement (10) with staging. Static and flow cytometric techniques have been employed, the first by means of densitometric study on paraffin sections with the Feulgen method, the second based on application of Hedley method on paraffin included material. DNA content, pressed by the DNA index, is inversely proportional to survival with similar results in both techniques, from which the possible prognostic significance is argued.

Carcinoma↗
PubMedDate not supplied

PNA: a marker of neoplastic progression and differentiation in the gastro-intestinal tract.

We examined 35 cases of stomach carcinoma and 40 cases of colonic carcinoma with PNA associated with peroxidase (peanut agglutinin, lectin which binds to the terminal disaccharide galactose beta (1,3)-N-acetil-galacto-samine). In this way evaluation of the functional aspects of the normal-neoplastic sequence was undertaken. This method was carried out for histological and ultrastructural investigations. The results obtained in both cases showed a different reactivity in the evolution of neoplastic disease: in fact, positivity in dysplasia is finely granular intracytoplasmic, whereas in well-differentiated neoplastic transformation such a reactivity is preferentially localized along the cellular membranes, with restoration of gross positivity in the cytoplasm for the poorly-differentiated neoplasm. We therefore believe PNA to be a marker not only of neoplastic progression but of differentiation as well: we also hypothesize it to reveal glycoprotein groups with possible antigenic power, involved in immunologic interactions between tumor and host.

Biomarkers, Tumor↗
PubMedDate not supplied

[The gastrointestinal tract in H.I.V. patients. Anatomopathologic aspects of opportunistic infections].

The symptoms of the Acquired Immuno-Deficiency Syndrome (A.I.D.S.) in the gastrointestinal tract are principally represented by neoplastic processes (Kaposi's sarcoma, lymphoma) and by opportunistic infections. These infections, for the diversity of bacterial, viral or parasitic etiological agents which are involved, ask for new and more specific interpretative problems, from the pathological point of view-either in the identification of the pathogenic agents either in the recognition of infective lesions associated with the presence of a not directly objectivable infective agent. On the basis of these considerations the authors report the principal histopathological characteristics connected with opportunistic infections of the gastrointestinal tract in 18 H.I.V. positive patients. Of every segment of the gastrointestinal tract, the detected agents are described with the lesions related to them, the other not specific A.I.D.S. associated infective forms recently described in literature, and in particular the differential diagnostic problems connected with viral infections. In the large bowel must be underlined the problems and the principal distinctive elements between opportunistic agents derived colitis and idiopathic colitis.

Acquired Immunodeficiency Syndrome↗