[Research in complementary medicine: results and problems].
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Clinical Trials as Topic↗
Biomedical subjects
P H Baumann
Publications and source records attributed to P H Baumann.
[Academic medicine and complementary medicine differ from each other in reasoning and evaluation but not in goals].
The principal objective of all medical activity is to prevent deterioration of health, but also its recuperation, when it has been lost, by mobilisation of inner, natural resources and the commitment of practical knowledge about ill-health. This is less true not for individuals with regard to their proper care, than for practitioners of the arts and sciences of healing towards those who seek their help, regardless of the 'school' to which they may belong. If such objectives are generally accepted, the way to their accomplishment is open. However relevant and justifiable differing intentions may be, they become difficult to materialize if energy is squandered in futile disputes over territorial rights. It is the purpose of the Swiss National Science Foundation's 'Komplementärmedizin' Programme (NFP 34) to build bridges between different territories. (See the Table of Projects currently being undertaken at the end of this article.)
Attitude of Health Personnel↗
Three-dimensional component labeling of digital confocal microscope images enumerates replication centers in BrdUrd labeled fibroblasts.
An algorithm (3Dlabel-1) for labeling three dimensional binary data sets has been developed. Serial optical sections are acquired using a confocal laser scanning microscope. After filtering and thresholding operations, contiguous elements in the three dimensional data sets are identified and labeled. The results are used to calculate the number and size of objects. Additionally, the labeled data are displayed by applying an algorithm (3Ddisp-1) to generate stereo pairs, in which label numbers are color coded and depth is cued by intensity. These procedures have been applied in investigations of the temporal and spatial distribution of replication centers throughout S phase in BrdUrd pulse labeled mouse fibroblasts.
Algorithms↗
Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts.
The temporal course of replication monitored by 2- or 5-min pulses of bromodeoxyuridine (BrdUrd) incorporation in synchronized 3T3 cells was mapped by high-resolution light microscopy employing a charge-coupled device (CCD) camera and a confocal laser scanning microscope (CLSM). The cells were labeled simultaneously with monoclonal antibodies directed against BrdUrd and nuclear lamin, and stained with the A+T-specific dye 4',6-diamidino-2-phenylindole (DAPI). Stereoscopic reconstructions of cells showing both the lamin and BrdUrd distributions demonstrate that DNA replication occurs at discrete sites in the nucleus, the locations of which progress through a programmed sequence during S phase. Replication begins in a small number of sites in the interior of the nucleus exclusive of the nuclear membrane and proceeds rapidly in early S phase to encompass a relatively large number of small, discrete sites located throughout the nucleus, with the exception of the condensed heterochromatic regions. Replication is primarily confined to the condensed heterochromatic regions in mid-to-late S phase, and to the nuclear periphery at the end of S phase. These distinctive patterns demonstrate a programmed control of replication sites in the spatial domain in differentiated cell nuclei.
Animals↗