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Biomedical subjects

P Halberg

Publications and source records attributed to P Halberg.

At least 19 recordsLinked to original sources
PubMed1979

Enzyme-linked immunosorbent assay for determination of IgM rheumatoid factor.

A semi-automated enzyme-linked immunosorbent assay (ELISA) for determination of IgM rheumatoid factor was established. Human gammaglobulin (Cohn fraction II) was used as an antigen on a solid phase of polystyrene microtitre trays. The results were read on a spectrophotometer. ELISA and the sheep cell agglutination test were compared in a study of sera from 400 blood donors, 53 patients with active rheumatoid arthritis and of 200 sera received for routine determination of rheumatoid factor. The results of the two tests correlated well. The ELISA procedure was precise, objective, inexpensive and well suited for quantitative routine determination of IgM rheumatoid factor.

Adolescent↗
PubMed1979

Deposits of immunoglobulins and complement in skin of patients with rheumatoid arthritis. Influence of anti-rheumatic treatment.

Immunopathological studies on skin biopsies from 88 patients with rheumatoid arthritis showed that one-third of them had deposits of IgM and/or C3 in the walls of small vessels immediately underneath the dermal-epidermal junction. The deposits in the vessel walls which may reflect subclinical immune complex vasculitis could be correlated to the occurrence of IgG-rheumatoid factor in the serum, but not to IgM-rheumatoid factor, other extra-articular manifestations, or to the occurrence of circulating immune complexes demonstrated by the complement consumption test or the thrombocyte aggregation test. Two untreated patients had granular deposits in the dermal-epidermal junction. Five out of 50 patients developed deposits in the dermal-epidermal junction during treatment with levamisole, penicillamine, or azathioprine, as observed by serial skin biopsies.

Anti-Inflammatory Agents↗
PubMed1978

A clinical study of 32 patients with oral discoid lupus erythematosus.

Thirty-two patients (26 females, six males) with discoid lupus erythematosus of the oral mucosa have been studied. Patients with signs of dissemination were classified according to accepted criteria. Four patients had systemic lupus erythematosus as well as oral discoid lesions. Twenty-one patients had discoid skin lesions. Eleven patients had oral discoid lesions without involvement of the skin. The time of onset of the oral discoid lesions rnaged from 6 to 75 years with a mean of 41 years. The mean druation of the oral lesions was 4.2 years. Symptoms from the oral discoid lesions were present in 75% of the patients. The clinical appearance varied and some lesions were difficult to differentiate from oral leukoplakia or from the lesions seen in oral lichen planus. The oral discoid lesions were most often seen on the buccal mucosa, the gingiva, labial mucosa and vermilion border. The oral lesions were infected by yeasts in more than half of thepatients.

Adolescent↗
PubMed1978

Antibodies against double-stranded DNA in patients with connective tissue diseases. Comparison between Crithidia luciliae kinetoplast immunofluorescence test and Farr technique.

Antibodies against double-stranded (ds) DNA were demonstrated by an immunofluorescence technique using Crithidia luciliae kinetoplast as antigen, and by means of the Farr technique. Both techniques were used simultaneously in 172 sera from patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), juvenile rheumatoid arthritis (JRA), temporal arteritis (TA) and from healthy controls. Comparable results were obtained with the two techniques. SLE patients with active disease had higher titres of IgA antibodies than patients with inactive disease. Of the patients with RA and JRA, 10% had significant titres of dsDNA antibodies. Patients with TA and normal controls had either no dsDNA antibodies in their sera or very low titres without complement-fixing properties.

Adult↗
PubMed1978

Penicillamine-induced polymyositis-dermatomyositis.

Two patients with classical rheumatoid arthritis developed myopathy during treatment with penicillamine. In both patients, electromyography and muscle biopsies were indicative of polymyositis. In one patient, muscle enzymes were not examined at the appropriate time; in the other patient muscle enzymes were raised. This latter patient also had a rash and, in the dermal epidermal junction of pathological skin, granular deposits of immunoglobulin and complement were found. The myopathy subsided in both patients after withdrawal of penicillamine.

Action Potentials↗
PubMed1978

Deposits of complement and immunoglobulins in dermal and synovial vessels in psoriasis.

Deposits of complement C3 and/or immunoglobulin were found in the vessel walls and/or at the dermal-epidermal junction in skin lesions of all of 11 patients with psoriatic arthritis and guttate psoriasis. Similar deposits were seen in 6 out of 15 patients with psoriasis vulgaris. Synovial tissue available from 2 patients revealed deposits in the vessel walls.

Complement C3↗
PubMed1978

Levamisole-induced hypersensitivity.

A 73-year-old woman with rheumatoid arthritis was treated with Levamisole, 150 mg per day, on 2 days a week. Her arthritis improved, but she developed a severely itching rash, and the treatment was stopped after 6 months. Penicillamine was subsequently given and tolerated without skin complications. 15 months after regular Levamisole was stopped, she was given a single dose of 150 mg which provoked fever of 40 degrees C and rash. Thirteen punch-biopsy specimens were examined by direct immunofluorescence microscopy. During the Levamisole treatment, granular deposits of IgG and C3 were found at the dermal--epidermal junction. Subsequently, the deposits disappeared, but reappeared after Levamisole challenge. The patient's leukocytes were exposed in vitro to Levamisole, and 36% of the total histamine content in the basophils was released. Our results provide further evidence that Levamisole can cause type-I as well as type-III hypersensitivity.

Aged↗
PubMed1976

Precipitating antibodies against Micropolyspora phaeni in sera from mushroom workers.

A new method for determining precipitating antibodies in allergic alveolitis is presented. The principle used is immunoelectrophoresis, either as a counterelectrophoresis or as a crossed immunoelectrophoresis with intermediate gel. Twenty-two mushroom workers without anamnestic signs of allergic alveolitis and 15 control persons were investigated. Twelve mushroom workers (55%) had precipitating antibodies as determined by crossed immunoelectrophoresis, and nine of these persons had antibodies demonstrated with counterelectrophoresis. Thus, the sensitivity seems greater in the crossed immunoelectrophoresis, but we find that the simpler counterelectrophoresis is an excellent screening procedure in patients suspected of allergic alveolitis. In the controls only one person had precipitating antibodies, though the antigen tested for Micropolyspora phaeni exists ubiquitously. It seems probable from this study that the antigenic load is important, although it is remarkable that no mushroom worker with precipitating antibodies against M. phaeni had ever experienced symptoms compatible with allergic alveolitis.

Actinomycetales Infections↗
PubMed1976

Cytostatic treatment of glomerular diseases. III. A double-blind cross-over study of the effect of cyclophosphamide report from a copenhagen study group of renal diseases.

Fifty patients with renal glomerular diseases entered a double-blind cross-over study on the effect of cyclophosphamide; 38 had received neither corticosteroids nor cytostatic drugs before joining the study. Cyclophosphamide was given for 4 months in doses decreasing from 3 to 1.5 mg/kg b.wt. Cyclophosphamide caused a 46% decrease in the 24-hour excretion of urinary protein and a decrease in serum creatinine within the normal range. Albumin, transferrin and IgA in urine, as well as albumin clearance and the sieving coefficient of albumin, changed parallel to the total urinary protein. The initial values of proteinuria and serum complement were of prognostic significance for the effect of cyclophosphamide in serum creatinine. We were unable to demonstrate a prognostic significance for the variables: clinical diagnosis, renal histology, arterial BP, initial values of serum creatinine and IgG, IgA and IgM in serum and urine. ESR appeared to be the most reliable acute phase reactant. No differences were found between the changes in renal histology during cyclophosphamide or placebo.

Administration, Oral↗
PubMed1975

Beclomethasone dipropionate aerosol treatment of hay fever. A dose-response investigation.

In a controlled, double-blind study 20 children and adults, suffering from summer hay fever, were treated intranasally with a daily dose of 200 mug, 300 mug or 400 mug beclomethasone dipropionate (Beconase, Becotide Nasal) or with placebo for 2 weeks during the hay fever season. No beneficial effect of the placebo treatment was observed. In patients treated with 200 mug and 300 mug beclomethasone dipropionate a day there was a moderate decrease in nasal symptom scores and in use of antihistamine tablets. As the results indicated 400 mug a day to have the most pronounced effect on nasal symptoms, this dosage is recommended for children as well as adults suffering from summer hay fever.

Administration, Intranasal↗
PubMed1975

Deposits of immunoglobulins and complement C3 in clinically normal skin of patients with lupus erythematosus.

Deposits of immunoglobulins and/or complement C3 were found in two-thirds of biopsies from clinically normal skin of 64 patients with systemic lupus erythematosus, as judged by well defined criteria. The incidence of deposits in clinically normal skin was identical in patients with and without clinical skin lesions. IgM was found more frequently in the deposits (88%) than was IgG (36%) but equally frequently in involved and uninvolved skin, whereas C3 was found more often in patients with skin lesions (59% in involved skin, 36% in uninvolved skin) than in patients without skin lesions (14%). The occurrence of IgG and IgA was less frequent than that of C3 but the distribution was similar to that of C3. In normal skin of 20 patients with chronic discoid LE, deposits were found in one. Deposits were found in the skin of one-third of patients with nuclear antibodies in their sera and symptoms compatible with SLE but with a score of symptoms too low to meet the criteria.

Adolescent↗