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Biomedical subjects

P Hellings

Publications and source records attributed to P Hellings.

14 recordsLinked to original sources
PubMed1996

Routine determination of flumequine in kidney tissue of pig using automated liquid chromatography.

A high-performance liquid chromatographic assay is described as a routine analytical method for the determination of flumequine (FLU) and its hydroxylated metabolite (OH-FLU) in pig kidney tissue. Kidney samples (2 g) containing FLU and OH-FLU were extracted by liquid-liquid extraction with ethyl acetate (10 ml). Analytical separations were performed by reversed-phase HPLC with fluorometric detection at 252 nm excitation and 356 nm emission under gradient conditions. The mobile phase was acetonitrile-2.7.10(-3) M oxalic acid in water (pH 2.5). The assay is specific and reproducible within the flumequine range of 0.050-2.5 micrograms/g and recovery at 0.050 microgram/g was 94.8%.

Administration, Oral↗
PubMed1995

Determination of fumagillin in muscle tissue of rainbow trout using automated ion-pairing liquid chromatography.

A high-performance liquid chromatographic assay is described as a routine analytical method for the determination of fumagillin in rainbow trout muscle tissue. Muscle tissue samples (1 g) containing fumagillin were deproteinized with 8 ml of an acetonitrile-water mixture (2:6, v/v). The extracts were purified with a Bond Elut Octyl C8 cartridge column, washed with a water-methanol mixture (95:5, v/v; 4 ml) and fumagillin was eluted with acetonitrile (1 ml). Analytical separations were performed by reversed-phase HPLC with UV detection at 351 nm under gradient conditions. The mobile phase was acetonitrile-0.005 M tetrabutyl ammonium phosphate in water (pH 7.8). The assay is specific and reproducible within the fumagillin range of 20-1000 ng/g and recovery at 20 ng/g was 69.2%. Sample preparation involves the use of a robotic sample preparation system. Gravimetric validation of all operations enabled Good Laboratory Practices to be observed.

Animals↗
PubMed1989

A case study of qualitative versus quantitative reviews: the maternal-infant bonding controversy.

This study used meta-analysis methodology to examine a series of studies in maternal-infant attachment. The results of this quantitative analysis were compared with those of a classic qualitative analysis of the same subject. The advantages and disadvantages of both quantitative and qualitative approaches to literature reviews are discussed and compared with quantitative and qualitative analyses of individual research studies.

Female↗
PubMed1975

A short-term intensive training program for pediatric nurse practitioners.

A pediatric nurse practitioner course has been described in which 30 nurse trainees spent six seeks in an intensive training program followed by six months of preceptorship with a pediatrician in their own agency. This short but intensive program had advantages of permitting the PNP student to retain her health care agency position during the intensive part of the program and of assuring her a position as a PNP once the course was completed. Evaluation of the students by written and practical examinations indicated that they had made significant gains in their knowledge and understanding of pediatrics and in their ability to evaluate and refer patients and to provide well-child care and maternal counseling.

California↗
PubMedDate not supplied

Breast-feeding problems and telephone consultation.

Parents and health care providers may have difficulty obtaining accurate and current information for the management of breast-feeding concerns. This study documents the types of problems that lead family members and health care professionals to seek telephone consultation from a breast-feeding service managed as an activity of a school of nursing faculty practice. During the 6-month study period, calls from 387 individuals (64% mothers and 31% health care providers) were received, for a mean of 64 calls per month. These calls required approximately 9.5 hours monthly to manage. Thirty-one percent of the calls were requests for general information (including stooling and urination or frequency and duration of breast-feeding); an additional 24% requested consultation from or referral to the service. Twenty-five percent of the calls resulted in a consultation with either the clinicians (9.9% inpatient; 9.6% outpatient) or referral to another health care professional (6%). Suggestions for pediatric nurse practitioner practice with breast-feeding mothers and babies were developed from the results of this clinical study.

Breast Feeding↗