Biomedical subjects
Publications and source records attributed to P J Kudirka.
This paper describes the performance of absorbance and fluorescence detectors in two configurations of post-column reactors for selectively detecting and quantitating isonenzymes eluted form a high-pressure liquid chromatography column. Superb resolution of the isoenzymes of lactic dehydrogenase is illustrated by the use of new ion-exchange materials.
We describe a totally automated, computer-controlled system for separating and measuring the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes. These isoenzymes in tissue extracts were separated on disposable DEAE-cellulose mini-columns. Resolution was complete except that LD-1 was not resolved from LD-2.
The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique.
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