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Biomedical subjects

P J Sharp

Publications and source records attributed to P J Sharp.

At least 55 records · Page 3Linked to original sources
PubMed1999

Effects of vasoactive intestinal peptide on plasma prolactin in passerines.

Vasoactive intestinal peptide (VIP) is a potent releaser of prolactin (PRL) in domestic fowl, turkey, and ring doves. However, few comparative studies have investigated this in wild species. We tested the effects of intravenously administered chicken VIP on plasma PRL concentrations in four passerine species: the white-crowned sparrow (Zonotrichia leucophrys gambelii), the dark-eyed junco (Junco hyemalis), the Florida scrub-jay (Aphelocoma coerulescens), and the western scrub-jay (A. californica). In the white-crowned sparrow, junco, and Florida scrub-jay, which were tested during the breeding season, VIP induced a rapid increase in plasma PRL. Serial plasma samples taken after VIP injection in the white-crowned sparrow show a 10-fold increase in PRL within 2 min of treatment, followed by a gradual decline. Effects of VIP, as compared to saline, remained significant for at least 20 min after treatment. Western scrub-jays did not respond to intravenous VIP with a significant rise in PRL secretion, possibly because they were tested after termination of the breeding season. This study indicates that VIP control of PRL release may be widespread among avian species, and that seasonal changes in plasma PRL may be mediated in part at the level of the pituitary. In addition, analysis of the control data revealed no increase in plasma PRL as a result of injection or restraint, suggesting that unlike in mammals, PRL is not released during acute stress in passerines.

Animals↗
PubMed1999

An analysis of physiological mechanisms underlying the antigonadotropic action of intracranial prolactin in ring doves.

Intracerebroventricular (ICV) injections of prolactin (PRL) exert potent antigonadal and antigonadotropic effects in ring doves (Streptopelia risoria) at doses that are insufficient to stimulate prolactin-dependent crop growth. To explore the physiological basis of these effects, we tested the ability of ICV-injected PRL to influence pituitary responsiveness to chicken gonadotropin-releasing hormone-I (cGnRH-I) and to alter GnRH content and concentration in the preoptic area (POA) and median eminence (ME). cGnRH-I-induced changes in plasma LH were monitored by radioimmunoassay (RIA) in photostimulated male doves after they received five daily ICV injections of ovine PRL (1 microg/2 microl) or saline vehicle. Although PRL treatment reduced basal plasma LH levels and testes weight, it did not reduce the amount or alter the pattern of LH released in response to a bolus injection of cGnRH-I. This suggests that ICV PRL does not suppress LH by reducing pituitary responsiveness to GnRH. In two subsequent studies, GnRH content (ng/region) and concentration (pg/microg protein) in the POA and ME were measured in male doves by RIA and by competitive enzyme immunoassay after 5 days of ICV PRL or vehicle treatment. Although ICV PRL reduced plasma LH levels in both studies, no significant PRL-induced alterations in GnRH content or concentration were apparent. In a final study, PRL-treated female doves had lower plasma LH levels than vehicle-treated control females at 12 and 24 h after a single ICV injection. GnRH content of the POA was also lower in PRL-treated females than in controls at 24 h. However, the two treatment groups did not differ in POA or ME GnRH content at earlier postinjection sampling intervals. Analysis of GnRH concentration data revealed no treatment group differences in either region at any sampling interval (1, 6, 12, or 24 h post-PRL injection). Collectively, these results are consistent with the idea that ICV-injected PRL acts at the level of the CNS to inhibit the reproductive system. However, the nature of the alterations involved remains to be clarified. Plausible hypotheses are (1) that ICV PRL suppresses the gonadal axis by influencing the activity of GnRH neurons at brain sites other than the POA or ME or (2) that PRL alters the synthesis, storage, degradation, and/or release of GnRH in the POA or ME, but the dynamic changes involved are not reflected in integrated, steady-state measures such as peptide content or concentration in tissue.

Animals↗
PubMed1999

The chicken leptin gene: has it been cloned?

The DNA sequence of a chicken leptin gene that shares 95% nucleotide similarity with the mouse leptin sequence has been recently reported (Taouis et al., 1998, Gene 208, 239-242). Experiments have been performed independently in two laboratories to try to confirm this finding. Fourteen PCR primers based on the mouse leptin sequence were designed to amplify the avian leptin gene. Four of the primers were identical to the mouse and published chicken leptin sequences. PCR amplification was carried out on genomic DNA and reverse-transcribed mRNA from the fat, liver, and pancreas of several chicken strains and from the domestic turkey, goose, and Japanese quail. No PCR products sharing close similarity to the mouse leptin sequence were generated from any avian templates. Amplification of mouse leptin sequence was consistently obtained when control mouse templates were used. Northern hybridization using a mouse leptin probe failed to produce a signal with poly(A)+ RNA from chicken fat and liver and from the fat and liver of force-fed geese but a strong signal was obtained from control mouse fat total RNA. Southern hybridization under low stringency washing conditions revealed hybridization of a mouse leptin probe to chicken genomic DNA. Under higher stringency washing conditions, the chicken signal disappeared, while those from control mouse and sheep genomic DNA remained. This suggests that the putative chicken leptin sequence shares less than the 83% nucleotide sequence identity between the mouse and sheep genes. It is concluded that a chicken leptin gene sequence with close sequence similarity to mouse leptin is not present in the chicken genome. Furthermore, mRNA sharing high sequence identity with mouse leptin is not present in the fat or liver of the domestic chicken, turkey, goose, or Japanese quail.

Animals↗
PubMed1999

Photo-induction of hypothalamic gonadotrophin releasing hormone-I mRNA in the domestic chicken: a role for oestrogen?

Photoinduced changes in GnRH neuronal function were investigated in prepubertal and in midpubertal cockerels and somatically mature hens. Photostimulation of short day mid-pubertal cockerels and somatically mature out-of-lay hens for 7 days significantly increased (P<0.05) total hypothalamic gonadotrophin releasing hormone-I (GnRH-I) mRNA. The increase in GnRH-I mRNA was associated with increased (P<0.05) plasma LH in the hens but not mid-pubertal cockerels. Photostimulation of short day prepubertal cockerels for 7 days also stimulated LH release (P<0.05) but in contrast did not increase total hypothalamic GnRH-I mRNA. Plasma LH and hypothalamic GnRH-I mRNA were depressed in (P<0.001) short day prepubertal cockerels chronically treated with oestradiol benzoate (0.5 mg/kg, on alternate days). However, photostimulation of oestrogenized prepubertal cockerels for 7 days stimulated LH release (P<0.001) and increased hypothalamic GnRH-I mRNA (P<0.001). It is concluded that photostimulatory inputs to GnRH neurones have the potential to increase GnRH-I mRNA transcription or stability and to increase GnRH-I release. The extent to which increased levels of GnRH-I mRNA or increased GnRH release from GnRH neurones are observed after photostimulation may depend on the interaction between the drive on GnRH-I neurone function, which increases at the onset of puberty, and the inhibitory action of oestrogen produced locally in the hypothalamus.

Animals↗
PubMed1998

Prolactin receptor gene expression in the brain and peripheral tissues in broody and nonbroody breeds of domestic hen.

The objective of this study was to establish whether the gene encoding prolactin receptor (PRLR) is expressed in the hypothalamus and peripheral tissues of the domestic chicken and, if so, to determine whether there are breed differences in the structure or expression of the gene which might account for the observation that broodiness does not occur in the White Leghorn hen but does occur in other breeds of domestic hens, including the bantam. A preliminary experiment demonstrated that the absence of broodiness in White Leghorns is not due to a lack of a prolactin response to the avian prolactin-releasing hormone vasoactive intestinal polypeptide. The largest amounts of PRLR mRNA in the brain, which did not differ significantly between laying White Leghorns and bantams, were found in the pituitary gland and basal and preoptic hypothalamus. Small or nondetectable amounts were found in both breeds in the forebrain, cerebellum, and optic lobes. Prolactin receptor mRNA was widely distributed in peripheral tissues in both breeds, in the following descending order of abundance: kidney, leg skin, brood patch, duodenum, intestine > thyroid gland > adrenal gland, liver, ovary >> adipose tissue > thymus, spleen > muscle > blood. Southern blotting analysis using four restriction enzymes and a chicken PRLR cDNA probe demonstrated identical digestion patterns for White Leghorn and bantam genomic DNA. Northern blotting analysis identified two sizes of chicken PRLR mRNA transcripts (7.5 and 3.3 kb) in hypothalami from laying White Leghorn and bantam hens. It is concluded that differences in the expression of broodiness in White Leghorn and bantam hens cannot be explained by differences in the amounts of PRLR mRNA in the hypothalamus or in the transcription or gross structure of the PRLR gene.

Animals↗
PubMed1998

Ontogeny of the photoperiodic control of prolactin and luteinizing hormone secretion in male and female bantams (Gallus domesticus).

The development of the photoperiodic responses for prolactin and luteinizing hormone (LH) secretion was determined in the bantam (Gallus domesticus), which becomes somatically mature at 16-18 weeks of age. Male and female bantams were reared from hatch on short days (8 h light/day) and photostimulated by transfer to 20 h light/day at 4, 8, 12, and 16 weeks. Photostimulation at all ages stimulated a robust increase in prolactin secretion in both sexes. Luteinizing hormone secretion was also stimulated except in 4-week-old females and 16-week-old males. It is concluded that the neural pathways involved in the photoperiodic control of LH and prolactin secretion are mature in juvenile bantams well before somatic maturity.

Aging↗
PubMed1998

Relationship between prolactin receptor mRNA in the anterior pituitary gland and hypothalamus and reproductive state in male and female bantams (Gallus domesticus).

The aim of this study was to test the hypothesis that prolactin may up- and down-regulate prolactin receptor gene expression in the anterior pituitary gland and hypothalamus respectively. Experiments were carried out in bantams (Gallus domesticus). Comparisons were made of concentrations of PRLR mRNA in the anterior pituitary gland and basal and preoptic hypothalamus in adult males and females held on long days (low vs high plasma prolactin); in 3-week-old juvenile male and females on short days (high vs low plasma prolactin); in 8-week-old juvenile male and females on short days (both low plasma prolactin); in adult laying, incubating, and out-of-lay (high, very high, and low plasma prolactin, respectively); in adult cockerels exposed to long or short days (high vs low prolactin); and in adult hens exposed to long or short days (high vs low prolactin). There was a sex difference in anterior pituitary and basal hypothalamic PRLR mRNA, with lower values in both tissues in females than in males. Compared with laying and out-of-lay hens, anterior pituitary and basal hypothalamic PRLR mRNA concentrations in incubating hens were increased and decreased, respectively. In adult birds of either sex held on long or short days, there was no difference in pituitary PRLR mRNA, while basal hypothalamic PRLR mRNA was lower on short days. PRLR mRNA in the preoptic hypothalamus was not affected by sex, reproductive state, or photoperiod. It is concluded that there is no consistent relationship between plasma prolactin, in the physiological range, and the concentration of PRLR mRNA in the anterior pituitary gland, basal hypothalamus, and preoptic hypothalamus.

Aging↗
PubMed1998

Influence of stimuli from chicks on behavior and concentrations of plasma prolactin and luteinizing hormone in incubating hens.

Removal of eggs from the nests of incubating birds or substitution of eggs for chicks disrupts incubation behavior and induces changes in the secretion of prolactin and luteinizing hormone (LH). The aim of the present study was to determine how different stimuli, such as physical contact with eggs and tactile, visual, and/or auditory cues from chicks, interact to control the transition between incubation and brooding and to induce changes in prolactin and LH plasma concentrations. Physical contact with chicks, in the presence or absence of eggs, induced brooding behavior and an immediate fall in plasma prolactin concentration and a gradual increase in LH concentration. Vocalizations, particularly clucking and food calls, increased rapidly while incubation and nest attachment disappeared slowly. No change in plasma prolactin or LH concentration was observed in incubating hens which could hear and see or only hear chicks. These incubating hens showed no interest in chicks and continued to incubate persistently. To conclude, tactile stimuli, alone or in combination with visual and/or auditory stimuli from newly hatched chicks, are the only cues that induce the transition from incubation to brooding and the associated decrease in prolactin secretion and increase in LH secretion.

Acoustic Stimulation↗
PubMed1998

The effect of exogenous testosterone on parental behavior, plasma prolactin, and prolactin binding sites in dark-eyed juncos.

Numerous studies have shown that parental behaviors are mediated by prolactin (PRL), while testosterone (T) interferes with their full expression. The limited data available suggest that reduced parental behavior induced by T is not mediated by reduced concentrations of plasma PRL. We hypothesized that T reduces parental behaviors by reducing PRL receptor binding activity at central neural sites that promote the expression of parental behaviors. To test this hypothesis we implanted male dark-eyed juncos (Junco hyemalis) with testosterone-filled or empty implants and measured T and PRL levels, paternal behavior, and specific binding of radio-labeled PRL at selected brain regions that have been implicated in the mediation of parental behaviors. Our findings concurred with previous studies in that T-treated males reduced their parental contributions, had higher levels of T, and had equivalent levels of PRL compared with controls. We found no differences in the capacity to bind 125I-oPRL in three brain regions previously implicated in the mediation of parental care in birds, i.e., the preoptic area, ventromedial nucleus of the hypothalamus, and paraventricular nucleus of the hypothalamus. Thus our findings do not support the hypothesis that T interferes with the expression of parental behavior by reducing PRL receptor binding activity at central sites.

Animals↗
PubMed1998

The effect of infusion of hypertonic saline on glomerular filtration rate and arginine vasotocin, prolactin and aldosterone in the domestic chicken.

Domestic fowl were infused for 60 min with isotonic saline followed by 90 min with hypertonic saline. Plasma electrolyte concentrations, osmolality and haematocrit were measured. Urine electrolyte excretion rates, osmolar output and urine flow rates were also monitored. From these results fractional excretions of electrolytes were calculated. The renal function markers inulin and rho-amino hippuric acid were infused to enable the measurement of glomerular filtration rate and plasma clearance of rho-amino hippuric acid, respectively. Plasma samples were also taken to assay for the hormones prolactin, aldosterone and arginine vasotocin. Plasma electrolytes and osmolality, fractional excretion of electrolytes and osmolar output all increased, while haematocrit decreased, throughout the experiment. However, no significant change was found in urine flow rate and little change was seen in glomerular filtration rate. The clearance of rho-amino hippuric acid, which provides an indication of renal plasma flow, increased during hypertonic saline infusion. Plasma concentrations of aldosterone and prolactin decreased during the experiment and plasma concentrations of arginine vasotocin increased. Infusion of hypertonic saline had no consistent effect on glomerular filtration rate, which may be due to conflicting influences of expansion of the extracellular fluid volume and increased plasma osmolality.

Aldosterone↗
PubMed1998

Endocrine and testicular changes in a short-day seasonally breeding bird, the emu (Dromaius novaehollandiae), in southwestern Australia.

Seasonal changes in testicular morphology and blood plasma concentrations of LH, testosterone, and prolactin are described for captive male emus in southwestern Australia. Testicular mass and testicular testosterone did not differ between the non-breeding (spring-summer) and the breeding (autumn-winter) seasons. Nevertheless, the testes obtained in the breeding season (May and August) were nearly two fold greater in mass than those collected in the non-breeding season (October and February). The highest testicular concentrations of testosterone were observed in February and lowest in October, while the values during the breeding season were intermediate. The patterns of histological changes in the testes also indicate that emus breed over the autumn-winter months. Tubule diameter was larger in the breeding season than in the non-breeding season, whereas the relative volume of the interstitium was larger in the non-breeding and smaller in the breeding season. Moreover, during the autumn and winter months, plasma LH and testosterone concentrations were high. Outside this period, in spring and summer, the concentrations of these hormones were low. Prolactin concentrations rose around the winter solstice, after the initial increases in plasma LH and testosterone. The end of the breeding season, in early spring, was marked by a gradual decrease in plasma LH concentrations but a rapid fall in testosterone concentrations. Prolactin concentrations continued to increase and peaked near the spring equinox, several weeks after the breeding season ended, and then decreased to reach baseline values by mid-summer. These testicular and endocrine changes are consistent with observations that the emu is a short-day breeder in southwestern Australia. Reproductive activity in the male begins soon after the summer solstice, well in advance of the development of suitable breeding conditions, and is then terminated in spring before food resources become limited by the onset of the dry season.

Animals↗
PubMed1998

Control of luteinizing hormone and prolactin secretion in birds.

In birds, the secretion of luteinizing hormone (LH) and prolactin is controlled by the releasing hormones, gonadotrophin releasing hormone-I (GnRH-I) and vasoactive intestinal polypeptide (VIP), respectively. The secretion of LH is depressed and the secretion of prolactin is at its highest, during incubation/brooding young and during the development of reproductive photorefractoriness. In incubating domestic chickens, decreased LH and increased prolactin secretion are correlated with decreased hypothalamic GnRH-I mRNA and increased hypothalamic VIP mRNA. Increased plasma prolactin contributes to the suppression of LH secretion in incubating poultry, possibly acting at the levels of the hypothalamus and the anterior pituitary gland. During the development of photorefractoriness, decreased plasma LH and increased plasma prolactin are correlated with decreased hypothalamic GnRH-I and increased hypothalamic VIP. In birds exposed to seasonal changes in daylength, the seasonally maximal concentrations of plasma prolactin associated with the development of photorefractoriness can be explained, in part, by the saturation daylength for photoinduced prolactin release being reached in late spring/mid summer. During the development of photorefractoriness, high concentrations of plasma prolactin play a role in ensuring the rapid regression of the gonads but are not essential for the maintenance of the condition.

Animals↗
PubMed1998

Effect of photostimulation on concentrations of plasma prolactin in castrated bantams (Gallus domesticus).

The annual breeding cycle of 'unimproved' breeds of domestic chicken, including the bantam, at temperate latitudes, is terminated by decreasing daylength in autumn and is initiated in late winter, while daylengths are still short. Observations on photoperiodic birds that terminate seasonal breeding by the development of long day photorefractoriness suggest that the photoinduced pattern of prolactin secretion is associated with the pattern of gonadal growth and regression. It was predicted that, if there is a causal relationship between photoinduced changes in prolactin secretion and gonadal function in birds then, in the bantam, the pattern of prolactin secretion observed after photostimulation would not be the same as in birds terminating breeding by the development of long day photorefractoriness. Experiments were carried out on surgically castrated bantams to avoid confounding the effects of photostimulation and the stimulatory actions of testicular hormones on prolactin secretion. Transfer of photosensitive castrated bantams from 8 to 14, 16, 18 or 20 h light/day initially stimulated prolactin release and, subsequently, after 20-30 days, concentrations of plasma prolactin progressively decreased. After 148 days of photostimulation, concentrations of plasma prolactin approached but were still higher than short day controls. Transfer of photosensitive castrated bantam cockerels from 8 to 12 h light/day stimulated a slower increase in plasma prolactin that subsequently remained higher than in other photostimulated groups. A further 4 h increase in photoperiod in the birds exposed for 148 days to 12 or 16 h light/day resulted, respectively, in a transitory increase and no increase in prolactin secretion. Recovery of photosensitivity for prolactin release was observed in the birds transferred to 18 or 20 h light/day for 148 days after treatment with 8 h light/day for 35 days. Attempts to obtain an independent hormonal correlate of the prolactin responses to photostimulation by measurement of plasma luteinizing hormone (LH) were unsuccessful. The concentration of plasma LH in castrated bantams did not change in response to a change in photoperiod. These observations show that the photoinduced pattern of prolactin release in the bantam, a species which terminates seasonal breeding in response to decreasing daylength, is the same as that in birds which terminate seasonal breeding by the development of long day photorefractoriness. It is concluded that the photoinduced pattern of prolactin secretion in birds can be dissociated from the neuroendocrine mechanisms controlling the termination of seasonal breeding.

Animals↗
PubMed1998

Behaviour and hormone concentrations in nest deprived and renesting hens.

1. Nest and egg deprivation is a procedure traditionally used to disrupt incubation behaviour expression in commercial flocks. The aim of this study was to establish how nest deprivation affects the subsequent changes in behaviour and hormone secretion and whether readiness to renest is related to hormone concentration before and during nest deprivation. 2. Incubating broiler breeder hens were deprived of their nest either in their familiar environment, by blocking the nest entrance in the home cage or in an unfamiliar environment, by transfer in a different cage. After 3 days of nest deprivation, next access was allowed and readiness to incubate tested. 3. Both methods of nest deprivation resulted in the expression of similar behaviours associated with the disruption of incubation, and similar increases in plasma LH and oestradiol and decreases in plasma prolactin. The percentage (approximately 70%) of hens which renested after either method of nest deprivation did not differ significantly. 4. Readiness to renest was not related to the concentrations of plasma prolactin measured before or during the 3 days of nest deprivation. However, hens which would later renest could be identified by using behavioural criteria. They emitted more avoidance trills before, and sat on the wire floor for longer periods during, the nest deprivation period than the others.

Animals↗