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Biomedical subjects

P Jakob

Publications and source records attributed to P Jakob.

23 records · Page 2Linked to original sources
PubMed1991

[Developmental problems of children, adolescents and young adults in families with an alcohol problem].

The situation of children and adolescents in families with an alcohol problem has been neglected in the mental health field. A role model is therapeutically beneficial, because "role" is a metaphor which implies that change can take place within a short period of time. These roles can be considered generalizations on various functions which present a bind to the alcoholism system for the children. Behavior which conforms to these roles impairs the formation of personal identity, individuation from the family of origin, and the development of stable relationships outside of the nuclear family. Therapy should aim at activating resources, rather than focussing on deficits.

Adolescent↗
PubMed1983

Diadenosine 5',5"'-P1,P3-triphosphate in eukaryotic cells: identification and quantitation.

A highly sensitive enzymatic assay for diadenosine 5',5"'-P1,P3-triphosphate (Ap3A) has been established on the basis of the coupled luminescence assay for diadenosine 5',5"'-P1,P4-tetraphosphate (A. Ogilvie (1981) Anal. Biochem. 115, 302-307). Snake venom phosphodiesterase splits Ap3A into AMP plus ADP which can be measured in a luminescence reaction containing pyruvate kinase, phosphoenolpyruvate and luciferin-luciferase. The procedure is linear with Ap3A levels ranging from 0.1 to 2 pmol. The assay has been used to measure Ap3A in various eukaryotic cells after ion-exchange chromatography and high-performance liquid chromatography of acidic extracts of the cells. The level of diadenosine triphosphate was higher in all instances than the level of diadenosine tetraphosphate. When growing in the abdominal cavity of mice, Ehrlich ascites tumor cells contained high amounts of Ap3A (0.1 nmol/10(6) cells), allowing direct optical determination in the HPLC chromatography. The quantitative measurement of Ap3A with the luminescence assay gave identical results. Ap3A extracted from Ehrlich cells was also chromatographed with authentic nucleotide in two thin-layer systems providing additional proof for the existence of Ap3A in biological material.

Adenine Nucleotides↗