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P K Rawlings

Publications and source records attributed to P K Rawlings.

3 recordsLinked to original sources
PubMed1976

Determining the base sequence of RNA molecules using continuous degradation kinetics.

A kinetic approach to sequence analysis is presented. A polymer chain composed of a small number of monomer types is degraded continuously with degradation proceeding in only one direction of the chain. Ordinarily, the direct determination of the sequence from the amount of degraded monomers is hampered by the rapid loss of reaction synchrony. However, it is shown, that if the monomers can be measured reasonably accurately, one can solve a system of linear equations with the unknowns giving the positions of the monomers in the chain, and the syn chrony loss due to the stochastic nature of the degradation process can be accounted for. Model calculations are presented.

Base Sequence↗
PubMed1976

Physical-chemical approach to the transient change in Na ion conductivity of excitable membranes.

A new method is proposed for analyzing the rapid transient current component (Na ions) in voltage clamp experiments on excitable membranes. The method is based on only two very general assumptions: the Na ion conductivity of an excitable membrane is determined by some general membrane parameter, the kinetic behavior of which is consistently described by the sum of only two simple exponential terms. A least square computer analysis for the data by L. Goldman and C.L. Schauf on Myxicola axons is described [(1973) J. Gen. Physiol. 61, 361-384]. The method gives (as a result) the relationship between conductivity and membrane parameter. A physically plausible, chemical model (cycle of three states) is proposed for a dissipative control of the Na ion conductivity. The rate constants for the specific model are calculated from kinetic parameters derived only from the general analysis. These rate constants reproduce the original voltage clamp data in every feature which includes peak current ratios (h infinity)-shift with test potential. By allowing for differences in the experimental conditions, we derive essentially the same rate constants for the voltage clamp data of A.L. Hodgkin and A.F. Huxley on squid giant axons.

Action Potentials↗