PubMed Health⌕ Search

Biomedical subjects

P K Storm

Publications and source records attributed to P K Storm.

10 recordsLinked to original sources
PubMed1994

[Respiratory problems, growth retardation and arthritis in turkeys and broilers caused by a Pasteurella-like organism: Ornithobacterium rhinotracheale or 'Taxon 28'].

Since August 1993 moderate to serious respiratory problems with necrotic pneumonia, growth depression and fast increasing mortality are seen in commercial turkeys (2-8 weeks of age) and broilers (4-6 weeks of age). An unidentified pleiomorphic Gram-negative rod was isolated from affected tissues. This Pasteurella-like organism, with yet unknown taxonomy, is recently named Ornithobacterium rhinotracheale gen. nov. sp. nov. or 'Taxon 28'. Experimentally severe growth depression and arthritis could be evoked in commercial turkeys and chickens. Respiratory signs caused by O. rhinotracheale could not (yet) be reproduced experimentally. This is the first report of the isolation of this organism in poultry in the Netherlands findings.

Animals↗
PubMed1994

Identification, purification, and characterization of a thiol-activated hemolysin (suilysin) of Streptococcus suis.

The present report describes the identification, purification, and characterization of a hemolysin produced by Streptococcus suis type 2. The hemolysin was purified from the culture supernatant by using different filtration steps, Superose-12 column chromatography, and selective (NH4)2SO4 precipitation. The purified hemolysin, designated suilysin, had an apparent molecular mass of 54,000 Da and exhibited a specific activity of 0.7 x 10(6) hemolytic units per mg. Suilysin appeared to belong to a family of toxins known as the thiol-activated toxins, with which it had several characteristics in common: loss of activity upon oxidation, reactivation upon reduction, and inhibition of activity by small amounts of cholesterol. The N-terminal amino acid sequence of suilysin showed many similarities with parts of the deduced N-terminal amino acid sequences of perfringolysin O, streptolysin O, listeriolysin O, alveolysin, and pneumolysin. Mice immunized with a vaccine containing purified suilysin appeared to be completely protected against a lethal S. suis type 2 challenge, indicating that suilysin is an important factor and that the neutralization of this single factor is sufficient to protect mice against the detrimental effects of an S. suis type 2 infection. Most of the different (serotype) strains appeared to secrete hemolytic activity which was biochemically and immunologically indistinguishable from suilysin into the culture supernatant in vitro, indicating that suilysin might be a cross-protection factor.

Amino Acid Sequence↗
PubMed1993

Identification of F11 fimbriae on chicken Escherichia coli strains.

Fimbriae were purified from Escherichia coli strains isolated from chickens with septicemia or colibacillosis. When grown on solid media, these strains expressed fimbriae with an apparent subunit molecular mass of 18 kDa. Morphological, biochemical, serological, functional, and molecular characterization revealed that these 18-kDa fimbriae are identical to F11 fimbriae, which were previously found to be involved in the pathogenesis of human urinary tract infection. Screening of a large strain collection showed that 78% of chicken E. coli strains expressed F11 fimbriae, whereas this percentage increased to 96% when the only strains taken into account were those with the serotypes most commonly encountered in avian colibacillosis (O1:K1, O2:K1, O35, and O78:K80). The prevalence of F11 fimbrial expression appeared to be independent of the country of isolation of the strains, except for the United States, where the prevalence seemed higher. Expression of F11 fimbriae by chicken E. coli strains could not be correlated with adherence to chicken tracheal or pharyngeal cells.

Animals↗
PubMed1992

Efficacy of a trivalent Haemophilus paragallinarum vaccine compared to bivalent vaccines.

The efficacy of a trivalent oil-adjuvant Coryza vaccine containing serotypes A, B and C of Haemophilus paragallinarum has been compared with that of a bivalent oil-adjuvant Coryza vaccine containing serotypes A and C and that of a commercially available, bivalent A1(OH)3-potentiated vaccine, containing types A and C. The trivalent vaccine, given at 10 and 17 weeks of age, provided the best protection. Even at 55 weeks after booster vaccination, chickens were still significantly protected, following severe challenge with either of the three serotypes of Haemophilus paragallinarum. Both bivalent vaccines did not protect against type B challenge. Furthermore the oil-adjuvant vaccines induced higher HI-A titers, which correlate with protection, compared to the A1(OH)3-potentiated vaccine. The results show that type B strains are pathogenic and constitute a distinct immunotype and thus a Coryza vaccine should contain three serotypes to obtain a broader protection against all serotypes.

Adjuvants, Immunologic↗
PubMed1984

A test in vero cell monolayers for toxin production by strains of Pasteurella multocida isolated from pigs suspected of having atrophic rhinitis.

Monolayers of Vero cells showed a morphological change after exposure to supernatants of certain porcine Pasteurella multocida cultures. It appeared possible to screen Pasteurella multocida isolates for their ability to produce toxin and to cause atrophic rhinitis in pigs. A close correlation with the guinea pig skin test was demonstrated.

Animals↗