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Biomedical subjects

P Kleemann

Publications and source records attributed to P Kleemann.

4 recordsLinked to original sources
PubMed1998

Variations of DNA damage in human lymphocytes after enflurane exposure in vitro.

The rate of DNA single strand-breaks in lymphocytes of 160 human donors were determined after enflurane (CAS 13838-16-9) exposure in vitro. The rate of DNA damage increased in relation to the exposed enflurane concentration. However, not every lymphocyte sample showed an increased rate of DNA damage; some samples showed an increased rate even after exposure to only 0.4 vol% enflurane and other samples showed no increased rate even after exposure to 4.0 vol% enflurane. After exposure to 0.6 vol% enflurane and more the increased rate of DNA damage is statistically significant. The DNA damage in the lymphocytes differs individually. The reasons may be genetic differences in DNA repair. In patients with DNA repair deficiencies anesthesia with enflurane may induce irreversible DNA damage.

Adolescent↗
PubMedDate not supplied

Secretion of neutral and acid DNases in cultivated human lymphocytes after incubation with DNA; possible consequences for inhalation anesthesia.

After incubation with DNA human lymphocytes release neutral and acid DNase activities into the culture medium; the release depends on DNA concentration and time of cultivation. The electrophoretic mobility of the released neutral DNase activity is in accordance with DNase I and the electrophoretic mobility of the released acid DNase activity with DNase II. The released DNase activities do not originate from dead cells and are not influenced by blast cell formation. The anesthetic halothane can inhibit the released neutral and acid DNase activities. Inhalation anesthesia can possibly disturb the correlation between DNA and DNases in human blood.

Anesthesia, Inhalation↗