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Biomedical subjects

P Klouda

Publications and source records attributed to P Klouda.

9 recordsLinked to original sources
PubMed1993

Class I and II HLA antigens in British patients with oral lichen planus.

The frequencies of human leukocyte antigens--HLA-A, HLA-B, HLA-C, HLA-DR, and HLA-DQ1--were determined in a group of 40 white British patients with oral lichen planus and compared with those of healthy controls. Alterations in the frequencies of several HLA antigens were noted. In particular, an increase in HLA-Bw57 and a decrease in the frequency of HLA-DQ1 were seen in the group with lichen planus. When different clinical subgroups of lichen planus were compared with the control group, significant changes were also noted in the frequencies of HLA antigens. This suggests that lichen planus may represent a heterogeneity of diseases and that HLA-Bw57 may predispose a person to lichen planus whereas HLA-DQ1 may be associated with resistance to it.

Adult↗
PubMed1991

A rapid solid-phase rosette-inhibition assay for the detection of Fc receptor (FcRII)-blocking alloantibodies [corrected].

A procedure for the detection of FcR-blocking alloantibodies is described which uses human B lymphocytes immobilised on plastic by poly-L-lysine. Antibodies which inhibited rosette formation between B lymphocytes or Daudi cells and ox erythrocytes coated with rabbit antibodies (EA) were detected in 10 out of 10 sera containing anti-HLA A2 antibodies and 3 out of 3 sera containing anti-HLA class II antibodies. Inhibition of rosette formation (EAI activity) was mediated by protein G-separated IgG. Analysis of rosette formation using these 13 sera and lymphocytes from 39 donors revealed that the degree of inhibition was bimodal; most sera were either clearly inhibitory or non-inhibitory in the assay. However, there was no correlation between inhibition of rosette formation (EAI activity) and lymphocytotoxicity. Four pairs of sera showed similar patterns of reactivity (r greater than 0.6, p less than 0.01; 2 x 2 chi-square test), and cells from 2 donors showed antithetical reactions with 12 of 13 sera (r = -0.86, p less than 0.001). These data suggest that the solid-phase rosette inhibition assay is a rapid and reproducible means of detecting antibodies reactive with non-HLA class I or class II antigens on human B cells.

B-Lymphocytes↗
PubMed1985

HLA polymorphisms in Saudi Arabs.

The HLA-A, -B, -C, -DR, Bf and GLO phenotypes of 109 unrelated Saudi Arab males have been determined. HLA-A and -B antigen frequencies were compared with data reported for European Caucasoids and various Arab populations. Most similarities in antigen frequencies were seen between Saudi Arab and Iraqi populations. A high frequency of Bw50 was observed in Saudi Arabs. The frequencies of HLA-DR antigens in Saudi Arabs were compared to European Caucasoids. HLA-DR7 was at high frequency in Saudi Arabs. Linkage disequilibria between alleles of HLA loci was examined. Many instances of previously reported antigen associations were seen in Saudi Arabs, together with a number of associations which have not been described elsewhere. HLA-Cw6-Bw50-DR7-BfS0.7 is suggested as being a common haplotype in Saudi Arabs.

Complement Factor B↗
PubMed1985

The acute phase response in acute anterior uveitis.

The acute phase response is part of the body's systemic response to inflammation. It consists of the synthesis and release of proteins participating in the inflammatory process. The acute phase protein serum amyloid A (SAA) was studied by serial measurement of its concentration in 57 patients with acute anterior uveitis. Patients were divided into two big groups on the basis of their SAA concentrations. Thirty-eight patients showed a transient elevation of SAA and were designated responders. In 19 patients SAA levels remained normal throughout the study and these patients were designated non-responders. Responders and non-responders were of similar age and sex distributions. The severity of the uveitis was equal in the two groups, and did not correlate with the SAA concentrations at presentation. Responders tended to have had fewer previous attacks of uveitis than non-responders. HLA typing showed that HLA B27 was more frequent in patients with recurrent disease than in those suffering their first attack. The majority of HLA B27 positive patients were responders. SAA concentration does not predict the severity of an attack of uveitis, but absence of an acute phase response may be associated with a greater tendency to recurrence.

Acute Disease↗