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Biomedical subjects

P Klyve

Publications and source records attributed to P Klyve.

7 recordsLinked to original sources

Isolation and in vitro propagation of human choroidal fibroblasts. Morphology and characterization of the cultures.

Subretinal ingrowth of choroidal fibroblasts is of importance in several clinical settings such as age-related macular degeneration or after traumas such as laser treatment and infection. In the present investigation we describe a method for isolation and propagation of human choroidal fibroblasts in vitro. Outgrowth from the outseeded choroid was studied by means of phase contrast microscopy, scanning electron microscopy and by immunohistochemical methods. The secondary cultures were found to contain almost only fibroblasts (more than 95% of the cells), some contaminating retinal pigment cells were also found. Choroidal fibroblasts contribute to the fibrovascular subretinal scarring process, and our cell culturing system seems to be a suitable tool for studying factors regulating the behavior of these cells in vitro.

Aged↗

[Contusion of the eyeball. Changes in trauma types and their treatment].

All journals of patients admitted to the Eye Department, Ullevål hospital with the diagnosis ocular contusion were examined during three periods in the course of the 24 years from 1968 to 1991. The distribution of age, sex and number of patients in the different periods remained almost unchanged. The hospitalization period decreased from six days in the first period to two days in the last period, but this did not increase the incidence of complications. The number of working traumas was reduced, and traumas by violence remained unchanged. There was a large percentage increase in sport traumas, caused mainly by a ball, and these now account for more than 1/3 of all contusions. The prognosis was good in most patients.

Accidents, Occupational↗

[Surgery of strabismus performed during topical anesthesia using adjustable sutures].

Retrobulbar, peribulbar and general anaesthesia for strabismus operations all share the disadvantage that they do not permit preoperative evaluation of the effect of the operation. To overcome this problem, we now operate selected cases in topical anaesthesia, using adjustable sutures. In addition to using adjustable sutures in recession, we have developed a method of adjustable resection. During a six month period, 70% of our grown-up patients were operated by this method. We find it a useful supplement to traditional strabismus surgery, also in reoperations and in operations on paretic muscles.

Anesthesia, Local↗

[Tear gas and eye injuries].

In the course of the last year, the Eye Clinic, Ullevål hospital has treated six cases of corneal injury caused by violent use of tear gas spray cannisters. In some cases there was total abrasion of the corneal epithelium, which could take several weeks to heal. We want to draw attention to this "weapon", the use of which seems to be spreading among young people.

Adolescent↗

Topical anesthesia and adjustable sutures in strabismus surgery.

Our experience with the use of topical anesthesia and adjustable sutures in a one-stage operation for treatment of strabismus is discussed, and selected cases are presented. In addition to using the method in recession, we have also developed a method for using adjustable sutures in resection of muscles. We have also found the method useful in reoperations and in operations on paretic muscles.

Administration, Topical↗