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P Kuang

Publications and source records attributed to P Kuang.

At least 37 records · Page 2Linked to original sources
PubMed1997

Effect of acupuncture on vasoactive intestinal peptide in ischemic cerebrovascular diseases.

BACKGROUND AND PURPOSE: Vasoactive intestinal peptide (VIP) appears to play an important role as a neurotransmitter or neuromediater in ischemic cerebrovascular diseases (ICVD). The effect of acupuncture, which is used in treatment of ICVD with good efficiency, on VIP has not been known. For finding the mechanism of acupuncture in treatment of ICVD and the effect of electro-acupuncture on VIP, the present study was performed. MATERIALS AND METHODS: 59 patients with acute ICVD were randomly divided into two groups. Electro-acupuncture and routine treatment were given in Group 1 (n = 29), and routine treatment was used alone in Group 2 (n = 30). The cerebrospinal fluid (CSF) and blood were taken before the beginning of treatment and after a course of treatment in both groups. The control group consisted of 38 cases of non-ICVD. VIP was measured by radioimmunoassay. RESULTS: The level of CSF VIP in patients with acute ICVD was significantly lower than that in the controls, while the levels of plamsa VIP showed no significant difference between the ICVD and control groups, and the level of CSF VIP was not significantly correlated with the level of plasma VIP. After acupuncture treatment, the level of CSF VIP was increased and showed no significant difference as compared with the control group. CONCLUSION: Acupuncture might alleviate the disturbance of metabolism of VIP in CNS.

Acupuncture Therapy↗
PubMed1997

ET-1 gene expression of rat brain during ischemia and reperfusion and the protective effect of radix Salviae miltiorrhizae.

Endothelin-1 (ET-1) gene expression of rat brain during ischemia and reperfusion as well as the effect of Radix Salviae Miltiorrhizae (RSM) were studied with in situ hybridization. It was found that ET-1 gene expression of cerebral cortex and caudate-putamen was markedly increased both in 24 hours of ischemia and 24 hours of reperfusion groups (P < 0.01, P < 0.05). In RSM-treated rats, although the ET-1 gene expressions of ischemia and reperfusion sides were also increased as compared with contralateral cortex and caudate-putamen, they were significantly lower in RSM-treated rats than those of controls (P < 0.05, P < 0.01 respectively). The present study indicated that RSM can partly inhibit ET-1 gene expression of cerebral cortex and caudate-putamen during ischemia and reperfusion. This may be one of the protective mechanisms of RSM on cerebral ischemia and reperfusion.

Animals↗
PubMed1996

Batroxobin accelerates lipid accumulation of peroxidized low density lipoprotein in mouse peritoneal macrophages.

Mouse peritoneal macrophages were incubated in DMEM medium with batroxobin (DF-521) to determine the effect of batroxobin on the internalization of peroxidized low-density lipoprotein (pox-LDL) by transmission electron microscopy. Although the morphology of the mouse peritoneal macrophages after incubation with DMEM, normal LDL (n-LDL) and n-LDL plus batroxobin was similar to that of the cells before incubation, they exhibited numerous cytoplasmic lipid droplets after incubation with pox-LDL for 4 h. After addition of batroxobin to the medium containing pox-LDL, the production of lipid droplets in the mouse peritoneal macrophages was tremendously accelerated. Batroxobin accelerates the phagocytosis and degradation of pox-LDL by macrophages.

Animals↗
PubMed1996

Studies of Qingyangshen (II): modulatory effect of co-treatment with qingyangshen and diphenylhydantoin sodium on rat hippocampal c-fos expression during seizures.

We have reported that, after KA treatment in rats, there was first a significant increase in hippocampal c-fos expression during acute seizures and then a long-term inhibition in hippocampal c-fos expression during chronic seizures. In this experiment, we examined the modulatory effect of Qingyangshen (QYS), a traditional Chinese medicine with antiepileptic property, and diphenylhydantoin sodium (DPH) on hippocampal c-fos expression during seizures. We found that after intraperitoneal injection of QYS + DPH (15 mg/kg and 50 mg/kg respectively, q.o.d. x 6), the peak level of hippocampal c-fos mRNA induction during KA-induced acute seizures was reduced by 56.27%; while the inhibition of hippocampal c-fos expression during KA-induced chronic seizures was disinhibited to control level. It is suggested that the inhibitory effect of QYS + DPH on KA-induced seizures, chronic seizures in particular, may have much to do with its modulatory effect on hippocampal c-fos expression.

Animals↗
PubMed1996

Changes of endothelin-1 gene expression in rat brains during ischemia and ischemic reperfusion.

OBJECTIVE: The experiment was designed to study the association of cerebral ischemia and reperfusion with endothelin-1 (ET-1) gene expression of rat brains and time-dependent changes of ET-1 gene expression during cerebral ischemia. MATERIALS AND METHODS: Thirty-three male SD rats were divided into dot blot hybridization (n = 27) and in situ hybridization groups (n = 6). The focal cerebral ischemia and reperfusion models were made with suture embolism of middle cerebral artery. Dot blot hybridization groups were redivided into control and ischemic subgroups (ischemia for 0.5, 1, 1.5, 3, 6, 12, 24, 48 and 72 h respectively). In situ hybridization groups were redivided into ischemia and reperfusion groups. After 24 h ischemia and 24 h reperfusion, ET-1 gene expressions were investigated with in situ hybridization and the results were analyzed with IBAS 2000 Image Analysis System. RESULTS: Dot blot hybridization showed that ET-1 mRNA of cerebral cortex and caudate-putamen was increased at 6 h of ischemia and reached peak at 24 h (3.9 and 3.7 fold respectively), and at 72 h of ischemia it remained at high levels (3.5 and 2.1 fold respectively). In situ hybridization showed that the levels of ET-1 mRNA of cerebral cortex and caudate-putamen were also markedly increased both in 24 h ischemia and 24 h reperfusion groups (P < 0.01, P < 0.05 respectively). CONCLUSIONS: ET-1 gene expression in focal ischemic brain tissue were markedly and progressively increased during cerebral ischemia and reperfusion and down-regulation of ET-1 gene expression may be a new approach to the treatment of ischemic cerebrovascular diseases.

Animals↗
PubMed1996

[The changes of vasoactive intestinal peptide somatostatin and pancreatic polypeptide in blood and CSF of acute cerebral infarction patients and the effect of acupuncture on them].

VIP, SS and PP which exist in gastrointestinal tract and CNS might be to play an important role in nervous system as neurotransmitters of neuromediaters. There have been a few of reports about their changes in plasma and CSF in ICVD. The effects of acupuncture, which was used in treatment of ICVD with good efficiency, on VIP, SS and PP have not been known. For researching their changes in ICVD and effects of electro-acupuncture on them, and finding the mechanism of acupuncture in treatment of ICVD, the study was performed. The levels of VIP, SS and PP in 64 patients with acute cerebral infarction were determined. The points of acupuncture were Quchi (LI 12), Waiguan (SJ 5), Huantiao (BG 30), and Zusanli (ST 36). The routine treatment included dextran, nicotinic acid, aspirin, dipyridamole and radix salviae miltiorrhizae composita. The CSF and blood were taken before the begining of treatments and after a course of treatment. The level of VIP, SS and PP were measured by radiommunoassay. Results showed the level of CSF VIP in the patients was significantly lower as compared with controls. The level of plasma SS in the patients was lower, but the difference was not significant as compared with controls, and level of plasma PP in the patients was significantly increased when it was compared with controls. After electro-acupuncture treatment, in patients with good efficiency, CSF VIP recovered to normal level and the levels of plasma and CSF SS were significantly increased, while the level of plasma PP had no significant change. The results suggest that acupuncture might regulate the disturbances of metabolism of VIP and SS in CNS.

Acupuncture Points↗
PubMed1996

Inhibitory effect of 764-3 on Ca2+ uptake in rat brain synaptosomes.

In an attempt to examine the effect of 764-3, a purified component extracted from Radix Salviae Miltiorrhizae (RSM), on calcium channel, Ca2+ uptake in rat brain synaptosome stimulated by high-K+ and Glutamate (Glu) was investigated by using radioisotope labeled 45CaCl2. It was found that 764-3 could decrease the uptake of Ca2+ induced by high-K+ (60 mM) and 0.1 mM glutamate in a concentration-dependent manner. The maximum inhibiting rates were 45.22 +/- 4.88% and 37.58 +/- 4.52%, respectively. The results indicated that neuroprotective effect of RSM on cerebral ischemic injury may be related to its blocking effects on calcium channel.

Animals↗
PubMed1996

Effect of radix Salviae miltiorrhizae on nitric oxide in cerebral ischemic-reperfusion injury.

It has been postulated that nitric oxide (NO) is produced in significant amount on reperfusion of ischemic brain and could cause brain edema and cell death. This study was undertaken in an attempt to determine whether Radix Salviae Miltiorrhizae (RSM) could reduce cerebral NO contents in 4-vessel occlusion rat model after 30 min of global ischemia and 15 min of reperfusion. There was a significant increase in cerebral NO level in untreated animals as compared with sham-operated ones. By pretreatment with RSM the cerebral NO concentration was reduced to normal level. The possibilities of neuroprotective role of RSM in cerebral ischemic-reperfusion injury were discussed.

Animals↗
PubMed1996

Radix Salviae miltiorrhizae treatment results in decreased lipid peroxidation in reperfusion injury.

In our pervious investigations we have found that Radix Salviae Miltiorrhizae (RSM) can reduce neurological deficits and ameliorate cellular damages, and its protective actions are associated with its effects on cerebral edema, monoamines, neuropeptides, neurotransmitter aminoacids, thromboxane A2, peroxidation and down-regulation of c-fos gene expression in ischemic animals. The purpose of present study was to investigate the protective effect of RSM against lipid peroxidation in 4-vessel occlusion and reperfusion rat model. We found that in RSM-treated rats the cerebral SOD activity is significantly increased, while the cerebral MDA level is significantly decreased when compared with untreated animals. The results suggested that RSM can reduce the lipid peroxidation and afford cerebroprotection against reperfusion injury.

Animals↗
PubMed1995

Protective effect of radix Salviae Miltiorrhizae Composita on cerebral ischemia.

The protective effects of Radix Salviae Miltiorrhizae Composita (RSMC) on cerebral ischemia in gerbils produced by unilateral ligation of left common carotid artery and on the development of ischemic cerebral edema were studied. It was found that the administration of RSMC resulted in a very significant decrease in neurological deficits and the time for exhibiting first signs of neurological deficit delayed compared with untreated animals. Although the values of specific gravity of fresh cerebral tissue on the ligated side of common carotid artery in untreated animals (1.0406 +/- 0.0039 SD) and in RSMC-treated animals (1.0453 +/- 0.0009) were decreased significantly compared to that (1.0468 +/- 0.0003) of sham-operated animals, the specific gravity of cerebral tissue in RSMC-treated animals was significantly heavier than that in untreated animals (t = 5.5022, P < 0.001), i.e. the administration of RSMC decreased the severity of cerebral edema. A significant negative correlation was found between stroke index and specific gravity, and it suggests that one of the protective mechanisms of RSMC might be related to its ameliorating cerebral edema.

Animals↗
PubMed1995

Anti-inflammatory effects of tianrong acupoint on blood vessels of dura mater.

Although Tianrong acupoint (SI 17) is used for migraine headache, its mechanism of action remains obscure. The effects of Tianrong acupoint therapy on neurogenically-mediated plasma protein extravasation (125I-BSA) in rat dura mater induced by electrical stimulation of the right trigeminal ganglion was studied. When the unilateral trigeminal ganglion was stimulated (5 Hz, 1.2 mA, 5 msec for 5 min), the ratio of stimulated side/unstimulated side (cpm/mg) was 1.6663 +/- 0.0217. The plasma extravasation was blocked by different kinds of treatment in different degrees. The order of the ratio was as follow: Tianrong acupoint therapy (1.0917 +/- 0.0266) and Tianrong electrical needle therapy (1.1281 +/- 0.0227) < Tianrong acupoint injection with normal saline (1.325 +/- 0.0444) < Quchi acupoint (LI 11) injection with prednisolonum (1.5284 +/- 1.1624). The results indicated that Tianrong acupoint therapy could inhibit the neurongenic inflammation on the affected side.

Acupuncture Points↗
PubMed1995

Studies of qingyangshen (I): Differential expression of hippocampal c-fos proto-oncogene during kainic acid induced acute and chronic seizures.

We previously reported that Qingyangshen (QYS), a traditional Chinese medicine with antiepileptic property, had therapeutic effect on kainic acid (KA) induced experimental seizures (see JTCM 13 (4): 281-286, 1993 for reference). To investigate the mechanisms underlying the anticonvulsant action of QYS, we analyzed the modulatory effect of QYS on rat hippocampal c-fos proto-oncogene expression during KA-induced epileptogenesis in this and the following paper. The expression of hippocampal c-fos gene during KA-induced seizures were examined first. Rats were intraperitoneally injected with kainic acid (KA, 12 mg/kg) and hippocampal c-fos mRNA level was determined by Northern blot analysis during both acute (within one day after KA injection) and chronic (15 days after KA treatment) seizures. A mild increase in hippocampal c-fos mRNA level was observed 30 min after KA injection (being 1.98 +/- 0.70 times of control level), which corresponded temporally to the occurrence of the first limbic seizures. There was an 11.02 +/- 3.33 fold maximal induction of c-fos mRNA at 2 h after KA administration, which remained relatively stable even when behavioral seizures continued to aggravate 4 h after KA treatment. Twelve hours after KA administration, c-fos mRNA in the hippocampus returned to control level when acute seizures began to gradually disappear. In contrast, the induction of hippocampal c-fos mRNA during chronic seizures was inhibited significantly, being reduced by 55.40% compared to control. This suggested that c-fos is in different functional states at acute and chronic stages of epileptogenesis induced by KA.

Animals↗
PubMed1994

The effect of radix Salviae Miltiorrhizae Composita on peroxidation of low density lipoprotein due to copper dichloride.

It is well known that plasma lipoprotein, particularly oxidized LDL, plays an important role in the pathogenesis of atherosclerosis and atherosclerotic diseases. We used oxidized LDL generated by incubating LDL from healthy persons with copper dichloride as a model to investigate the antioxidate property of Radix Salviae Miltiorrhizae Composita (RSMC). On photos, the spot X1 and the spot X2 were clearly found in the control group after the dialysis into copper dichloride for 24 and 48 hours, but they could not found in the RSMC group. The analysis of the constituents of lipids in LDL (by charring method) showed that after dialysis the percentages of the spot X1 and the spot X2 in the RSMC group were significantly lower than those in the control group (P < 0.01). The results suggest that RSMC plays a potential role in antioxidation of lipids or LDL.

Adult↗
PubMed1994

The effect of radix salviae miltiorrhizae composita and ligustrazine on lipid peroxidation in low density lipoprotein due to copper dichloride.

It is well known that lipid peroxide in low density lipoprotein (LDL) plays an important role in atherosclerosis and atherosclerotic diseases. We used oxidized LDL generated by incubating LDL from healthy persons with copper dichloride as a model to investigate the anti-lipid-peroxide property of Radix Salviae Miltiorrhizae Composita (RSMC) and ligustrazine. The changes in concentrations of lipid peroxide and lipids in LDL due to Cu++ were studied, and the effects of RSMC and ligustrazine on the changes were studied. The results in our study indicate that RSMC has a potential role on anti-lipid-peroxidation, but it was not found that ligustrazine has similar anti-peroxidation action.

Adult↗
PubMed1994

Effect of radix salviae miltiorrhizae on EAA and IAA during cerebral ischemia in gerbils: a microdialysis study.

The concentrations of amino acids (AA), stroke index and infarct area were determined in 26 gerbils which were divided into 3 groups: RSM-treated (n = 8), Saline-treated (n = 10) and sham-operated (n = 8). The levels of AA were measured with microdialysis technique in cerebral cortex. The concentrations of neurotransmitter AA, as Glu and GABA and Asp, were significantly increased during the first 60 min after CCA ligation, while the concentrations of non-neurotransmitter AA, as Thr and Ser, had no significant changes. In RSM-treated gerbils, the level of Glu was significantly lower than that of the saline-treated, but the GABA in RSM-treated was significantly higher than that of the saline-treated. The ratio of Glu/GABA was significantly decreased after ischemia. The RSM could improve the reduction of ratio of Glu/GABA during 0-30 min and 91-120 min after cerebral ischemia. There were statistically significant decrease in terms of stroke index in RSM-treated group when compared with saline-treated group at 24 h and 16 h after CCA ligation respectively. The RSM has a tendency to decrease the size of infarct area, but no statistical difference. The results suggest that the neurotransmitter AA involve in the pathophysiological procedures of cerebral ischemia and the RSM can attenuate dysfunctions of EAA and IAA. Furthermore, the results also imply that there may be an alternate way to treat cerebral ischemia by inhibiting the presynaptic releasing of Glu and stimulating the releasing of GABA.

Amino Acids↗
PubMed1993

Evidence for amelioration of cellular damage in ischemic rat brain by radix salviae miltiorrhizae treatment--immunocytochemistry and histopathology studies.

The changes of somatostatin (SS)--like immunoreactive cells and morphology were observed in the models of ligation of right MCA and bilateral ligation of CCA respectively. The results showed: (1) a decline of SS immunoreactive nerve cell profiles in right cerebral cortex after 3h ligation of MCA as compared with left cerebral cortex and sham-operated group, while no significant difference was found between RSM-treated and saline-treated groups; (2) selective ischemic cell changes in cerebral cortex, hippocampus and caudate nucleus after 3h ligation of CCA. The changes included shrinkage and condensation of perikaryon, nuclear pyknosis, cytoplasmic eosinophilia in some neurons. The ischemic changes were more severe in saline-treated group than RSM-treated group. The experiments indicated that the selective ischemic cell changes could be improved by RSM, suggesting that treatment to modulate SS metabolism may be indicated after ischemic insults.

Animals↗
PubMed1993

Effect of qingyangshen on hippocampal alpha- and beta-tubulin gene expression during kainic acid induced epileptogenesis.

Using alpha- and beta- tubulin cDNA Probes and Northern blot hybridization technique, we analyzed the effect of intraperitoneally injected Qingyangshen (QYS), a traditional Chinese medicine with antiepileptic property, and diphenylhydrantoin sodium (DPH) on hippocampal alpha- and beta-tubulin gene expression during kainic acid (KA) induced chronic seizures. It was found that: 1) thirty days after intraperitoneal injection of KA, alpha- and beta-tubulin mRNAs in animals showing chronic seizures increased 3.02 +/- 1.05 and 4.07 +/- 1.32 times respectively compared with control; 2) neither QYS (15 mg/kg, q.o.d. x 6) nor DPH (50 mg/kg, q.o.d. x 6), when used separately, could inhibit the above mentioned effect of KA; 3) when QYS (15 mg/kg, q.o.d. x 6) and DPH (50 mg/kg, q.o.d. x 6) were given in combination, the long-term increase in tubulin gene expression induced by KA was significantly reduced, with alpha- and beta-tubulin mRNAs being decreased to 0.44 +/- 0.08 and 0.50 x 0.10 times of corresponding values in animals treated with KA alone. The results indicate that the mechanism of antiepileptic effect of QYS is at least partially related to the inhibition of tubulin synthesis and subsequent reduction in mossy fiber sprouting and neosynaptogenesis.

Animals↗