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Biomedical subjects

P Kupcsulik

Publications and source records attributed to P Kupcsulik.

32 records · Page 2Linked to original sources
PubMed1979

Ischemic damage of the liver. Part I: In vitro investigation of the prevention of the ischemic lesion of the liver.

In an isolated rat liver perfusion system the effects of normothermal ischemia on hepatic functions were investigated. After 30 minutes of anoxy bile production and BSP elimination capacity of the liver are significantly reduced. The quantity of secreted "ascites" from the surface of the liver several times high after anoxic damage, while oxygen consumption, portal venous pressure and ammonia elimination do not differ significantly from the controls. Pretreatment with insulin plus glucose, isoproterenol, hypoxanthine, chlorpromazine and glucagon (5 micrograms/100 g i.v., or 0.2 mg/100 g s.c.) does not reduce noticeably the normothermal anoxic lesion of the liver Glucagon (50 micrograms/100 g i.v.), allopurinol, dibenzyline, ATP-MgCl2 and aspartic acid enhance significantly the ischemia-tolerance of liver in vitro.

Animals↗
PubMed1979

Ischemic damage of the liver. Part II: In vivo investigation of the prevention of the ischemic lesion of the liver.

A new model for the study of ischemic liver lesion on rats has been worked out. Pretreatment with allopurinol, dibenzyline, methylprednisolone, glucagon, ATP-MgCl2 and aspartic acid reduced the overall mortality of ischemic liver injury. Administered after the anoxic hepatic lesion only glucagon and aspartic acid had beneficial effect on the survival rate. Under the influence of 30 minutes of normothermal ischemia the DNA synthetizing ability of the liver decreased. Aspartic acid, glucagon and ATP-MgCl2 significantly enhanced the regeneration of the ischemically damaged liver. These procedures might be suitable for donor pretreatment in liver transplantation, as well as for the treatment of other pathological states, causing a normothermal ischemia of the liver.

Adenosine Triphosphate↗
PubMed1979

[The effect of ischemia on ascites secretion in the perfused rat liver].

The ascites fluid secreted by the isolated perfused liver was studied in rats. The quantity of fluid appearing on the surface of the isolated perfused liver was independent of the pressure in the portal vein and could be raised by the amount of fluid flowing through the liver. Irrespective of the flow conditions, the ischaemic liver will produce more ascites than the intact liver und similar conditions. A rise of hepatic oncotic pressure will not reduce ascites production by the ischaemic liver. With respect to osmolarity there is no difference between the perfusing fluid and ascites. As regards the quantity of bilirubin secreted with ascites no significant difference was found between the control and the ischaemic group. The pertaining literature and the theories of ascites formation are discussed.

Animals↗
PubMed1977

Effect of ischaemia on the enzyme activity of the hepatic tissue.

The lactate and pyruvate levels, as well as acid and alkaline phosphatase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, glutaminic acid-oxalacetic acid transaminase and aldolase levels of rat liver homogenizates were measured at 24 degrees C and 38 degrees C during 120 min ischaemia from 0 to the 120th min. With the exception of transaminase and aldolase, the other enzymes were also enzyme-histochemically studied. The early lesions of the liver can be detected, both the quantitative laboratory tests and enzyme histochemical studies. The deviations from normal, observed at 24 degrees C between the 60th and 100th min, and at 38 degrees C between the 30th and 60th min, might be signs of irreversible lesions. Fractionated study of the liver homogenizate improves the assessability of enzyme determinations. In the course of "warm" ischaemia, the liver lysosomal lesions are early symptoms. Parallel to the breakdown of aerobic glycolysis lactic acid, fermentation, and an active pentose phosphate cycle can be detected. Quantitative testing of the liver homogenizate and enzyme histochemical observation of the hepatic tissue, might be a suitable method for the assessment of ischaemic liver lesions.

Acid Phosphatase↗
PubMed1976

Enzyme histochemical studies of the preserved rat liver.

The effect of depersolone, of the oxygenation of the perfusing solution and of phenoxybenzamine pretreatment has been studied in the isolated rat liver intermittently perfused with a solution containing low molecular weight dextran at 4 degrees C. The use in liver preservation of Collins' C3-solution and of a special albumin-containing solution was tested. The behaviour of acid and alkaline phosphatase, esterase, lactate dehydrogenase and adenosine triphosphatase in the preserved liver was followed by means of histochemical methods allowing semi-quantitative evaluation. Pretreatment with phenoxybenzamine and perfusion by an albumin-containing solution reduced the lesion of the liver, while prednisolone and oxygenation of the perfusion solution improved preserving effect only moderately.

Acid Phosphatase↗
PubMed1975

[Enzyme-histochemical studies of ischemic damage to the pancreaticoduodenal graft ready for transplantation in the dog].

The ischaemic lesion of the pancreaticoduodenal graft prepared for transplantation was studied by enzyme histochemical methods. Estimation of lactate dehydrogenase, non-specific acid and alkaline phosphatases and esterases and of glucose-6-phosphatase pancreas. While in the pancreas, lesions due to warm ischaemia will appear after one hour, the same can be observed in the duodenum after 30 minutes and in both organs the lesions progress rapidly. Lesions resulting from cold effects appear in the pancreas after three hours and remain unchanged for 15 to 18 hours, while in the duodenum they will appear after one hour and deteriorate considerably after three hours. Continuous perfusion for more than 30 minutes or the glucose perfusion test performed after cold ischaemia of more than two hours duration considerably affected the enzymes of the organs.

Acid Phosphatase↗
PubMedDate not supplied

Occurrence and significance of Helicobacter pylori infection after gastric surgery.

Occurrence and significance of Helicobacter pylori (Hp) infection in patients having undergone partial and total gastric resection was studied. Forty-nine patients after gastric resection with Billroth I and II operation (28 patients due to ulcer and 21 patients due to gastric cancer), and 8 patients with total gastrectomy due to gastric cancer were selected. In addition, 40 patients with active peptic ulcer disease, 32 patients suffering from gastric cancer, and 28 patients with non-ulcer dyspepsia as a control group were enrolled. Occurrence of Hp in patients having been operated on due to ulcer disease was slightly lower than in controls (50% vs. 54%), but it was significantly lower than in patients with active peptic ulcer (50% vs. 85%, p < 0.001). On the other hand, in patients who were operated on due to gastric cancer, Hp was much less frequent than in the control group, as well as in patients in the preoperative period (9.5% vs. 54% and 72%, p < 0.001). As a conclusion, since in our series colonization with Hp was found to be less frequent after gastric resection than before surgery, its aetiologic significance in postoperative chronic gastritis and ulceration seems to be unlikely. Therefore, the routine postoperative eradication of Hp appears to be unnecessary in such patients.

Adult↗