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Biomedical subjects

P L Cary

Publications and source records attributed to P L Cary.

13 recordsLinked to original sources
PubMed1996

Prevalence and implications of perinatal substance use in Missouri.

A study was conducted to determine the prevalence of perinatal substance use in Missouri. The population sample studied was selected according to a multi-stage probability-proportional-to-size sampling. The weighted prevalence for perinatal exposure to alcohol, tobacco or illicit substances was 31.9%, 10.8% for illicit substances, 21.9% for tobacco use, and 7.9% for self-reported alcohol use. In 1993, an estimated 23,925 perinatal exposures to licit and illicit substances occurred.

Adult↗
PubMed1985

Evaluation and comparison of a radiative energy attenuation method for determining cholesterol in serum.

Determination of cholesterol by a radiative energy attenuation (REA) technique was evaluated and compared with results obtained by the Boehringer Mannheim High Performance Cholesterol Assay and the Du Pont aca. Within-assay and between-assay CVs for the REA method, for two sets of controls, were both less than 5%. We observed no interference with lipemic samples. Analytical recovery averaged 102.8%. We used all three methods for parallel determinations of 217 patients' samples containing a wide range of cholesterol concentrations. Linear regression analysis of the REA results vs those of the comparison methods were as follows: REA = 1.03 Boehringer - 0.072 (r = 0.993) and REA = 1.02 aca - 0.048 (r = 0.995). We also discuss bilirubin interference with the REA method for cholesterol.

Adolescent↗
PubMed1984

Acetaminophen overdose with fetal demise.

A case of fetal demise and maternal recovery after acetaminophen overdose is presented, to our knowledge the first reported. Fetal liver and maternal serum concentrations indicate overdose to be the cause of fetal death. Maternal disseminated intravascular coagulation (DIC) may have been related to maternal acetaminophen-induced liver disease alone or to a combination of liver disease and the presence of a dead fetus.

Acetaminophen↗
PubMed1984

Abbott radiative energy attenuation method for quantifying ethanol evaluated and compared with gas-liquid chromatography and the Du Pont aca.

Quantification of ethanol by a radiative energy attenuation (REA) technique was evaluated and compared with results by gas-liquid chromatography (GLC) and by the Du Pont aca. Within-assay CVs were less than 5.5%. Between-assay CVs ranged from 1.9% to 6.0% for serum and blood controls at concentrations of 0.5, 1.0, and 2.5 g/L. We observed no cross reactivity with methanol, isopropanol, or acetone, and analytical recovery of ethanol from serum averaged 101%. For the three-method comparison we performed parallel determinations of 156 blood, 92 serum, and 54 urine samples containing a wide range of ethanol concentrations. Linear regression analysis of the REA results vs those of GLC or aca yielded the following: for serum, REA = 1.03 GLC -0.03 (r = 0.998), REA = 1.13 aca -0.04 (r = 0.999); for blood, REA = 0.97 GLC + 0.05 (r = 0.994), REA = 0.99 aca + 0.06 (r = 0.996); and for urine, REA = 1.01 GLC -0.03 (r = 0.998). We also discuss the clinical and forensic use of the REA method for ethanol.

Alcohol Dehydrogenase↗
PubMed1983

Quantitation of 3'-hydroxypentobarbital in serum using high-performance liquid chromatography.

A quantitative method for the determination of 3'-hydroxypentobarbital (3'HP) in serum has been developed using reversed-phase high-performance liquid chromatography. A sensitivity limit of 0.5 micrograms/ml in 100 microliters serum was obtained by detection at 215 nm. Serum concentrations of 3'HP were measured in patients receiving high-dose pentobarbital therapy and pentobarbital:3'HP concentrations are discussed.

Chromatography, High Pressure Liquid↗
PubMed1983

Reactivity of EMIT serum barbiturate assay reagents: potential application for the quantitation of selected barbiturates.

The reactivity of EMIT serum barbiturate assay reagents toward barbiturate test compounds was investigated. Reactivity was determined by the C5 substituents on the barbiturate ring and O/S substitution (barbituric/thiobarbituric acid derivatives). Reagents were more reactive toward a 5,5-substituted barbiturate than toward its hydroxylated analogue. EMIT reactivity could be linearized vs. concentration of test compound, allowing for quantitative analysis in serum or plasma.

Barbiturates↗
PubMed1983

Pentobarbital quantitation using EMIT serum barbiturate assay reagents: application to monitoring of high-dose pentobarbital therapy.

Pentobarbital serum concentrations associated with a high-dose therapeutic regimen were determined using EMIT immunoassay reagents. Replicate analyses of serum controls resulted in a within-assay coefficient of variation of 5.0% and a between-assay coefficient of variation of 10%. Regression analysis of 44 serum samples analyzed by this technique (y) and a reference procedure (x) were y = 0.98x + 3.6 (r = 0.98; x = ultraviolet spectroscopy) and y = 1.04x + 2.4 (r = 0.96; x = high-performance liquid chromatography). Clinical evaluation of the results indicates the immunoassay is sufficiently sensitive and selective for pentobarbital to allow accurate quantitation within the therapeutic range associated with high-dose therapy.

Cross Reactions↗
PubMedDate not supplied

A rapid method for the identification of acidic, neutral, and basic drugs in postmortem liver specimens by Toxi-Lab.

A rapid, qualitative method for the identification of drugs and drug metabolites in postmortem liver specimens has been developed using the Toxi-Lab drug screening system. A single-step liquid-liquid extraction, using heptane/dichloromethane, was employed for acidic/neutral drugs. Basic/neutral drugs were extracted with 1-chlorobutane and subjected to back-extraction and lipid clean-up. Chromatographic separation and drug identification required no further modifications of the Toxi-Lab procedure. The method, including tissue preparation, requires approximately 2 hr, is compatible with GLC analysis, and utilizes minimal sample size. The assay allows detection of 5 to 20 micrograms of drug/g of tissue. The use of this assay in forensic analyses is discussed.

Chromatography, Gas↗
PubMedDate not supplied

Immunoassay method validation for a modified EMIT phencyclidine assay.

Immunoassay drug testing methods, that have been modified from the manufacturers' recommended procedure to be used for the analysis of federally regulated specimens or other forensic samples require evaluation to ensure their scientific validity. These validation studies must demonstrate the accuracy, precision, and linearity of the modified immunoassay around the cutoff concentration, substantiate adequate rate separation, and verify the ability of the assay to differentiate positive and negative samples. Modification of the EMIT d.a.u. phencyclidine assay in order to achieve the federally mandated cutoff concentration of 25 ng/mL is common. This study describes the validation of a modified EMIT phencyclidine assay and a protocol that allows for the evaluation of similarly modified immunoassays.

Calibration↗
PubMedDate not supplied

Automated extraction for the analysis of 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (THCCOOH) in urine using a six-head probe Hamilton Microlab 2200 system and gas chromatography-mass spectrometry.

An automated extraction scheme for the analysis of 11 -nor-delta9-tetrahydrocannabinol-9-carboxylic acid using the Hamilton Microlab 2200, which was modified for gravity-flow solid-phase extraction, has been evaluated. The Hamilton was fitted with a six-head probe, a modular valve positioner, and a peristaltic pump. The automated method significantly increased sample throughput, improved assay consistency, and reduced the time spent performing the extraction. Extraction recovery for the automated method was > 90%. The limit of detection, limit of quantitation, and upper limit of linearity were equivalent to the manual method: 1.5, 3.0, and 300 ng/mL, respectively. Precision at the 15-ng/mL cut-off was as follows: mean = 14.4, standard deviation = 0.5, coefficient of variation = 3.5%. Comparison of 38 patient samples, extracted by the manual and automated extraction methods, demonstrated the following correlation statistics: r = .991, slope 1.029, and y-intercept -2.895. Carryover was < 0.3% at 1000 ng/mL. Aliquoting/extraction time for the automated method (48 urine samples) was 50 min, and the manual procedure required approximately 2.5 h. The automated aliquoting/extraction method on the Hamilton Microlab 2200 and its use in forensic applications are reviewed.

Automation↗