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P Lépine

Publications and source records attributed to P Lépine.

At least 19 recordsLinked to original sources
PubMed1975

[RNA-fragments, necessary primers for the in vitro replication of DNA].

Partial degradation of ribosomal RNAs (E. coli rich in purine nucleotides) by different ribonucleases gives rise to the appearance of several families of RNA-fragments which after separation on "Sephadex G 25" were analyzed for base ratio, size and biological activity. In the presence of DNA dependent DNA polymerase, RNA-fragments act as primers for in vitro replication of DNAs from numerous sources.

Carbon Radioisotopes↗
PubMed1975

[RNA polymerase activity associated with a bunya virus (Lumbo)].

RNA dependent RNA polymerase has been demonstrated in purified Lumbo virus (Bunyavirus) which contains a single stranded segmented RNA. Divalent cations (Mn++ and Mg++) are required for optimal in vitro activity. Reaction products can be specifically annealed with the viral genome.

Arboviruses↗