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Biomedical subjects

P Labbe

Publications and source records attributed to P Labbe.

At least 55 records · Page 3Linked to original sources
PubMed1976

Some properties of yeast mitochondria prepared by a rapid mechanical procedure.

A method is described for preparing yeast mitochondria rapidly (within one hour) by using the MSK Bronwill Cell homogenizer. Yeast mitochondria obtained by the method exhibit relatively good respiratory controls and ADP/O ratios. The method is convenient for small or large amounts of yeast cells (from 5 to a hundred grams, wet weight) and gave a yield of 3 to 5 mg protein/g wet weight of yeast mitochondria.

Cell Fractionation↗
PubMedDate not supplied

Ferric iron reduction and iron assimilation in Saccharomyces cerevisiae.

We have used the yeast Saccharomyces cerevisiae as a model organism to study the role of ferric iron reduction in eucaryotic iron uptake. S. cerevisiae is able to utilize ferric chelates as an iron source by reducing the ferric iron to the ferrous form, which is subsequently internalized by the cells. A gene (FRE1) was identified which encodes a protein required for both ferric iron reduction and efficient ferric iron assimilation, thus linking these two activities. The predicted FRE1 protein appears to be a membrane protein and shows homology to the beta-subunit of the human respiratory burst oxidase. These data suggest that FRE1 is a structural component of the ferric reductase. Subcellular fractionation studies showed that the ferric reductase activity of isolated plasma membranes did not reflect the activity of the intact cells, implying that cellular integrity was necessary for function of the major S. cerevisiae ferric reductase. An NADPH-dependent plasma membrane ferric reductase was partially purified from plasma membranes. Preliminary evidence suggests that the cell surface ferric reductase may, in addition to mediating cellular iron uptake, help modulate the intracellular redox potential of the yeast cell.

Cell Membrane↗
PubMedDate not supplied

Recruiting and retaining medical technologists.

Personnel recruitment and retention is one of the most challenging facets of management. Despite turnover rates, identifying and retaining good employees should be foremost on any managerial agenda. An effective employee recruiting/relations program enhances the overall productivity of an operation by addressing concerns at staff level, often considered the core of activity. In this issue, we asked our respondents: What methods do you use to recruit and retain medical technologists?

Laboratories, Hospital↗
PubMedDate not supplied

[Anthropometric study of the face in childhood from birth to 11 years (author's transl)].

The normal values of some facial characteristics have been established in 1,130 French children from birth to 11 years in the Lille area. Included in the study were: distances between the internal and external angles of the eye, interpupillary distance, length of the palpebral fissures, height, width and depth of the nose, width and height of the lips and of the external ear. No significant difference could be found in boys and girls.

Age Factors↗