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Biomedical subjects

P Lazar

Publications and source records attributed to P Lazar.

At least 19 recordsLinked to original sources
PubMed2004

Protein A/G dependent ELISA a promising diagnostic tool in Lyme disease seroprevalence in game animals and hunting dogs.

One of the major problems in serodiagnosis in wild animals is unavailability of specific antiglobulin conjugate. Our study focuses on validation of Protein A/G dependent ELISA in game animals like deer and mouflons as well as in hunting dogs. Binding ability of Protein A/G-conjugate to antibodies was the highest in dogs followed by fallow deer and mouflons. Three different whole cell Borrelia antigens were used to evaluate antigen dependent variation. In new Protein A/G-ELISA the highest sensitivities (90.50%, deer; 85.37%, mouflon & 94.29%, dog) were obtained by B. garinii antigen, with no statistically significant variation (chi(2), P>0.05) among all other antigens used. Average seroprevalences observed in deer, mouflons and dogs were 44.90%, 29.41% and 30.43%, respectively. Marked influence of age on seroprevalence was noticed. Protein A/G-ELISA proved to be sensitive and promising diagnostic tool in serodiagnosis of Lyme disease in game ungulates and it can be used effectively for serosurvey in different wild mammals.

Age Factors↗
PubMed2001

Development and in vitro efficacy of novel MMP2 and MMP9 specific doxorubicin albumin conjugates.

Two doxorubicin albumin conjugates (A-DP1 and A-DP2), which differ in their substrate specificity for the matrix metalloproteinases MMP2 and MMP9, were prepared by binding maleimide doxorubicin peptide derivatives to the cysteine-34 position of human serum albumin. The incorporated octapeptide, Gly-Pro-Gln-Arg-Ile-Ala-Gly-Gln, in A-DP2 is not cleaved by activated MMP2 and MMP9 in contrast to Gly-Pro-Leu-Gly-Ile-Ala-Gly-Gln incorporated in A-DP1 that is cleaved efficiently by activated MMP2 and MMP9 liberating a doxorubicin tetrapeptide. A-DP1 showed antiproliferative activity in a murine renal cell carcinoma line in the low micromolar range (IC(50) value approximately 0.2 microM).

Albumins↗
PubMed1996

[Follicular maturation, dizygotic twin conceptions and maternal age].

This note aims to given an interpretation of the "paradoxical" increase of dizygotic twin pregnancies (and more generally of multiple pregnancies) according to maternal age, when in parallel the number of growing follicles decreases strongly. From the analysis, through an allometric model, of the relative variations with age of the size of the various ovarian follicular compartments, it reinforces the hypothesis of the existence of an inhibition factor, whose level would depend on the size of the residual stock of follicles and which would act on one or several phases of the follicular development. It brings evidence for the major role that this factor might play in the regulation of the whole reproductive life of women (and probably other mammals).

Abortion, Spontaneous↗
PubMed1994

Marker-derived phylogeny of European cattle supports demic expansion of agriculture.

Using frequencies of 86 genes from 23 loci of blood group systems, blood and milk proteins, the genetic relationships among 14 cattle breeds including four native Balkan and four synthetic Balkan-Alp breeds were studied. The dendrogram and nonlinear map construction shows a consensus 'Balkan breed cluster', an 'Alp breed cluster', an unstable position of synthetic breeds and well-separated American breeds. Positive partial correlations between genetic distance and time elapsed since introduction of farming while keeping geographical distances constant, and regular patterns over thousands of kilometers indicate that large-scale cattle population movements together with human migration (in the Neolithic age) from the Near East into Europe across the Balkans are the most likely explanation for the genetic distances observed in our data. More recent breed differentiation and selection do not yet blur this initial pattern of European cattle populations.

Agriculture↗
PubMed1991

The action of calcitonin gene-related peptide on astrocyte morphology and cyclic AMP accumulation in astrocyte cultures from neonatal rat brain.

The actions of calcitonin gene-related peptide (CGRP) were investigated on 9- to 14-day-old astrocyte cultures obtained from the cerebra of 1- to 2-day-old rats. The morphological appearance of the cells was monitored by immunofluorescent staining of glial fibrillary acidic protein (GFAP), and the concentration of adenosine 3',5'-monophosphate (cyclic AMP) was measured in the cultures using radioimmunoassay. CGRP induced a morphological change in cultured astrocytes from rat brain. Flat, polygonal cells positive for GFAP became multipolar with many processes. In addition, CGRP stimulated the accumulation of cyclic AMP by up to 30-fold, half maximal stimulation being obtained at CGRP concentrations in the range 100-300 nM. These data provide evidence for the presence of functional CGRP receptors on astrocytes and suggest a role for CGRP as an intercellular messenger controlling the state of differentiation and activation of astroglial cells.

Animals↗
PubMed1991

Dry skin, water, and lubrication.

Lubrication of the skin will become more important as our population ages. Many excellent moisturizers are available for lubrication. Individual ingredients that are touted heavily are not as important as the total mixture. The single most important factor is the regular use of the products to prevent overdrying of the skin. How should we use this information to achieve our aims? 1. The frequency of bathing, showering, and cleansing should be reduced. The water should be kept at the coolest temperature practical, and one should get out as soon as possible. Sponge bathing should be encouraged. Bath oils make tubs slippery and are wiped off by towels. It is better to apply an emollient directly on skin that is still damp. 2. Room temperatures should be kept low and comfortable and humidity should be kept as high as possible without causing damage. 3. Overdrying should be avoided by limiting the exposure to soap, detergents, solvents, and water. In addition, friction from washcloths, rough clothing, and abrasives should be avoided. 4. Emollients should be used frequently and selected to be compatible with the individual's situation. Bedridden individuals should use petrolatum, whereas the ambulatory would be better served by the use of lotions and creams. 5. If itching is present or reticulated cracking (eczema craquelé) is seen, more aggressive treatment is indicated, including possible use of topical steroid preparations. 6. A preparation that is cosmetically acceptable, so that it will be used regularly, should be prescribed. One must emphasize its importance so that the individual avoids serious and chronic dermatitis.

Cosmetics↗
PubMed1990

[Preliminary results of noncontact temperature measuring using an infrared thermometer on the tarsus pf swine with osteoarthrosis tarsi deformans].

The surface temperature on tarsus of 54 performance tested boars of about 60 kg of Swedish Landrace was measured by means of infra-red thermometer at three measuring points (MP1, MP2 and MP3). MP1 was situated above the tuberculum of os metatarsale III or, as visualised in 21 slaughtered boars, above pathoanatomical alterations of osteoarthrosis tarsi deformans (OATD). MP2 was located directly medially and MP3 laterally from MP1. A clear palpatory symptom was found only with 10 boars, while all 21 slaughtered boars showed different morphological lesions of OATD. The average temperature at MP1 was 35.69 degrees C and was significantly higher (P = .01) than at MP2 (35.34) and MP3 (35.30). The temperature differences between MP1 and MP2 or MP3 were statistically reliable. Boars, who had a clear palpatory symptoms at tuberculum, showed an average temperature at MT1 of 36.27 degrees C, which was higher (P = .0114) than of those with an unclear palpatory symptom (35.64 degrees C). The results show that the temperature differences are the consequence of OATD.

Animals↗
PubMed1990

Body cavities in fishes.

Four types of body cavity arrangement were found in 20 species of fish (see Materials and Methods). The body cavities of fishes were also compared with those of mammals and birds. It was concluded that higher vertebrates utilized only the Tinca type for their evolution, while the other types were dead-end evolutionary forms which terminated at fish level, or possibly with amphibians and reptiles. The necessary research in these groups still remains to be done.

Animals↗
PubMed1988

Streaky pigmentation in a patient with acquired immune deficiency syndrome (AIDS).

We report a case of hyperpigmentation due to bleomycin treatment in a patient with acquired immune deficiency syndrome (AIDS). Although this type of hyperpigmentation has been previously seen in patients with cancer who are receiving bleomycin, this is, to our knowledge, the first reported case of bleomycin-induced hyperpigmentation in an AIDS patient and should be added to the growing list of cutaneous eruptions seen in these patients.

Acquired Immunodeficiency Syndrome↗