Biomedical subjects

P Lekić

Publications and source records attributed to P Lekić.

5 recordsLinked to original sources

PubMed1990

[Antibody titer in saliva and serum of experimental animals].

This paper reveals the antibody titer of plaque microorganisms in experimental gingivitis rats of differents ages and immunization. Before sacrificing experimental animals status of gingival tissue was assessed. Further on speciments of saliva and serum were taken for antibody titer measurements. None of the young rats developed gingivitis during experiment, where as the adult immunized rats bled on probing. Low values of antibodys point out the absence of intensive synthesis of specific antibodys in saliva upon the antigen stimulation of plaque maicroorganisms. Serum antibody titers were low in young rats, moderate in adult, and high in adult immunized rats. These results indicate that adult rats react stronger to plaque antigents than young rats and that previous contact with the antigens increases the reaction.

Actinomyces↗

PubMedDate not supplied

[Local and systemic immune response during experimental gingivitis].

The paper deals with the intensity of local and systemic immune response to the antigen stimulation of dental plaque in experimental gingivitis. Three groups, each of 11 rats, were in contact with S. mutans, F. nucleatum, A. viscosus and B. gingivalis in order to stimulate gingival inflammation. Experimental days were 0, 3rd, 7th and 14th day. Before sacrificing experimental animals condition of gingival tissue was assessed. Gingival specimens were then taken for numeric density analysis as well as serum for antibody titer measurement. None of the young rats developed gingivitis during the experiment, whereas the adult immunized rats bled on probing. The greatest increase of the number of lymphocytes occurred in the elder immunized group and the lowest in the group of one month old rats. Serum antibody titers were low in young rats, moderate in adult and high in adult immunized rats. These results indicate that adult rats reacted stronger to plaque antigens than young rats and that previous contact with the antigens increased the reaction.

Age Factors↗

PubMedDate not supplied

[Relation between dental plaque fluoride content and caries].

The purpose of this study was to investigate the effect of plaque fluoride content on dental caries in children aged 12 years, from the area with minimal (Valjevo) and optimal and/or higher fluoride concentrations (Vranjska Banja) in drinking water. The caries prevalence was determined by the standardized epidemiological method. The fluoride concentration in dental plaques was measured with the Ion-selective electrode. The fluoride content in the plaque of children from Valjevo (7.8 ppm) was significantly smaller than in children from Vranjska Banja (36.5 ppm). In 12-year old individuals with the lowest fluoride concentrations in the plaque the greatest values of the caries prevalence were found. The increase in plaque fluoride content provoked the decrease in caries prevalence. The lowest values of caries index were found in children with 21 to 30 ppF in dental plaque.

Child↗

PubMedDate not supplied

[Isoelectric focusing of mixed saliva proteins in individuals with HGH caries prevalence].

The protein content of mixed saliva was analysed in patients with low, mid and high caries prevalence by isoelectric focusing in different pH gradient on Servalyt plates. Determination of the protein fractions in pH-3-6.gradient points out a significant increase of glycoprotein fractions of pH = 4.7. The most basic fractions of this range (pH = 5.9) are disappearing within the high caries prevalence. Isoelectric focusing in pH-6-9 gradient points out that with the increase of caries prevalence there is an increase in protein fractions of pH = 8.3. Protein fractions of lysosomal prigin, pH = 10.65, are disappearing with the increase of caries prevalence in all analysed specimens. IEF method on Servalyt plates and polyacrylamid gel enables analysis of the Ca-precipitable acid and base like glyco-and phosphoprroteins (pHi = 4 and 8.3) which are of significance in maintaining physiological properties of the soft and hard teeth tissues.

Adult↗

PubMedDate not supplied

[Relation between plaque index and gingival cell infiltrate in experimental animals].

The aim of this study was to determine the relation between plaque and gingival cell infiltrates in highly imbreaded laboratory rats during experimentally provoked gingivitis. In this experiment 24 Lewis rats, aged 1-3 months, were used. From the zero day of inflammation plaque was scored by Sillnes and Löe index, while the presence of gingival cell infiltrate was determined with the stereological method. The results point out that the elder animals manifested early plaque accumulation, while the young group of animals presented a more intensive gingival cell infiltration. On the basis of the obtained results it can be concluded that within the equal amounts of plaque the young animals had a more intensive gingival cell infiltration as the result of distincet defensive reaction to the presence of microbial antigens.

Age Factors↗