Biomedical subjects
Publications and source records attributed to P Lemkin.
This overview of a hospice volunteer program discusses the many aspects of running such a program, including finding volunteers, providing training and education, keeping volunteers motivated, and offering bereavement education.
Managed care organizations often are unaware of the services that are unique to hospice--for example, the contribution of volunteers, the need to enroll patients at an appropriate stage in their illness, and the potential cost savings of dying at home rather than in the hospital. Hospices need to undertake educational campaigns to help ensure their inclusion in a managed care system.
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Informal caregivers--usually family members--take on a lot when they care for a patient. Professionals need to be aware of potential stress and recognize caregiver burnout. There are several ways agencies can help these caregivers.
As home care expands its capabilities, more conditions, such as heart disease, will be treatable at home. Home cardiac care encompasses many conditions, from short term to terminal.
Expression of smooth muscle alpha-actin and migratory behaviour of cloned cerebral endothelial cells (cEC) which exhibited two distinct phenotypes (type I, type II) were studied. Removal of mitogenic factors (alpha ECGF, ECGS) and heparin from the culture medium resulted in a smooth muscle-like appearance (type II) of the cells, expression of smooth muscle alpha-actin protein and smooth muscle actin mRNA and in an increased migratory activity. In contrast, addition of growth factors and heparin led to a cobblestone-like phenotype (type I) which lacked the expression of smooth muscle alpha-actin but expressed other proteins as determined by 2-D-gel electrophoresis.
We sought polypeptide markers of pathways of lymphoid differentiation by comparing the protein-synthesis patterns of a pair of autologous human lymphoblastoid cell lines: the B-cell line CCRF-SB and the T-cell line CCRF-HSB-2. Cells were cultured in standard medium for 24 or 120 h, then biosynthetically radiolabeled with [3H]leucine. Whole cell lysates were subjected to two-dimensional gel electrophoresis and patterns of protein synthesis were made visible by fluorography. Replicate cultures showed highly reproducible patterns, but differing culture durations produced alterations in the relative synthetic rates of some proteins. Protein synthetic patterns were analyzed visually and with the GELLAB system for computer analysis of two-dimensional gel images. Qualitative and quantitative differences were found between the T- and B-cell patterns. GELLAB detected and accurately paired most of the well-defined spots in each image and constructed a unified data base from data on our initial gels. Statistical analysis of this data revealed several marker proteins, the relative synthetic rates serving to distinguish the T-cell line from the B-cell line. Such computerized analysis of protein synthetic patterns of malignant cells appears likely ultimately to provide important diagnostic information regarding human malignancies.
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This paper presents an example of a distributed monitor system BMON2, which was developed and is in daily use in a biological image processing environment. Some useful aspects of such a system are discussed, particularly those which make for easier biologist-user interaction and system extensibility. The principles of extension of the distributed monitor to a time-shared computer system is outlined.
Computerized techniques for the evaluation of O'Farrell two-dimensional electrophoretic gels have been applied to proteins derived from asbestos bearing macrophages. Preliminary results indicate definite changes in the protein content of cells depending on fiber phagocytosis.
Two-dimensional electrophoresis in polyacrylamide gel provides a highly reproducible display of biosynthetically pulse-labeled cellular polypeptides, whose relative rates of synthesis can be judged from the relative spot densities they produce in fluorographic images of the electrophoreses. When experimental and control cell populations synthesize protein at differing rates, either the sample load per gel or the duration of exposure of gel to film may be adjusted to produce comparable experimental and control images with no alteration in the rank order of relative spot density. However, variation in the duration of pulse-labeling may significantly alter relative spot density. Film density saturation and nonlinear response to radiation may also alter relative spot density for polypeptides with very large or very small amounts of radioactivity. Stimulation of human peripheral blood lymphocytes with phytohemagglutinin alters the relative synthesis rates of certain polypeptides visualized on the gels. These can be quantitated by using an automated series of computer algorithms to analyze the images. Data from this analysis confirm statistically significant alterations in the relative densities produced by certain polypeptides. Various human lymphoblastoid cell lines may also be distinguished from each other by this technique.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.