Street smarts.
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Acquired Immunodeficiency Syndrome↗
Biomedical subjects
P Loftus
Publications and source records attributed to P Loftus.
The use of high resolution carbon-13 NMR in the study of mucus.
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Animals↗
The use of natural-abundance high-resolution carbon-13 NMR in the study of mucus glycoproteins.
Natural-abundance 13C NMR was used to study samples of native mucus from dog trachea and purified mucus glycoprotein from hog stomach. Despite the large molecular weight of the molecules (several million) and visco-elastic nature of the gel, spectra were produced which could be resolved into individual sharp resonances which gave significant structural detail. These results indicate the potential use of this powerful technique in the study of mucus glycoprotein structure in the undegraded molecule.
Animals↗
Nitrogen-15 nuclear magnetic resonance of aliphatic tripeptides.
The 15N chemical shifts of eight aliphatic tripeptides have been measured at the natural-abundance level. For a given tripeptide, the resonances of the COOH-terminal and NH2-terminal amino acids can be identified by measurements at low or high pH. The shifts of the NH2-terminal amino acid nitrogens are essentially independent of the amino acids in the rest of the peptide. The shifts of the other nitrogens are characteristic of the amino acids themselves and of the immediately preceding amino acid toward the NH2 terminus. Non-terminal amide nitrogens have shifts of about 6 ppm upfield of COOH-terminal amide nitrogens at the isoelectric point of measurement. 15N chemical shifts appear to have considerable potential value for peptide sequencing.
Amino Acid Sequence↗
Operation clean-up. Safety network.
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Cross Infection↗
Metabolism of tracazolate. Metabolites in dog and rat urine.
The urinary metabolites of tracazolate [4-n-butylamino-1-ethyl-6-methyl-1H-pyrazolo (3,4-b) pyridine-5-carboxylic acid ethyl ester], an anxiolytic agent, obtained from rats and dogs dosed with 14C-labeled tracazolate have been characterized. No unchanged tracazolate was detected. Fifteen metabolites were identified in dog urine, seven of which had not previously been found in rat blood and tissue. Eleven of these metabolites were also found in rat urine. The metabolites were formed by deesterification to the 5-carboxylic acid; N-deethylation of the pyrazole ring: oxidation at the gamma-position of the n-butylamino side chain; oxidation of the terminal carbon of this side chain; loss of the n-butylamino group; and hydroxylation of the 6-methyl group followed by condensation with the 5-carboxylic acid to form gamma-lactones. The major metabolites in dog urine were the desethyl-desbutyl-deesterified compound, the desbutyl-deesterified compound, and the desbutyl-desethyl-lactone. Loss of the butyl side chain and, also, lactone formation, appeared to occur to a lesser extent in the rat than in the dog.
Animals↗