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Biomedical subjects

P M Lundin

Publications and source records attributed to P M Lundin.

At least 19 recordsLinked to original sources
PubMed1985

Liver affection in iron overload studied with serum ferritin and serum aminotransferases.

Liver dysfunction as measured by S-ALAT activity was present in 72% of patients over 40 years of age with HLA-related iron overload, mainly detected by laboratory screening. Liver dysfunction was correlated to the amount of iron stored (r = 0.54, p less than 0.001). When iron was removed by phlebotomy, liver function returned to normal. S-ALAT activity was closely correlated to serum ferritin concentration (r = 0.73, p less than 0.001). Even a mild iron excess can affect hepatocytes and result in increased levels of ferritin and aminotransferases in serum. Patients with "transaminitis" should be investigated for iron overload.

Adult↗
PubMed1984

The effect of steroids on the circulating lymphocyte population--VI. Studies of the thoracic duct T- and B-lymphocyte populations after neonatal thymectomy and prednisolone treatment. An immunofluorescence study in the rat.

The corticosteroid effect on circulating lymphocytes in a steroid-sensitive species (rat) was studied by immunofluorescence. Both normal and neonatally thymectomized animals were injected with a high dose of prednisolone. T- and B-lymphocytes as well as T-lymphocyte subsets were analyzed in thoracic duct lymph during the involution phase (3 h after injection) and the restitution phase (17 h after injection) using conventional and monoclonal antisera. Neonatal thymectomy significantly reduced the number of circulating T-helper and T-non helper cells. In the involution phase, after a corticosteroid injection, there was a 64% reduction of lymph cell content in normal rats, affecting both T- and B-lymphocytes. Thymectomized animals showed an even more pronounced disappearance of cells. Counted in percentage, more B- than T-lymphocytes disappeared from the circulation. The T-non helper cells disappeared to a greater extent than the T-helper cells did. Cells with Ia-antigen showed about the same values as the B-lymphocytes. During the restitution phase all cells types were restored to normal levels. It seems that a high prednisolone dose has a more pronounced effect on the circulation of B- than of T-lymphocytes, and the T-non helper cells are probably more sensitive than the T-helper subpopulation. These data support the hypothesis that a major corticosteroid effect is a trapping and redistribution of circulating lymphocytes, more pronounced for certain cell types.

Animals↗
PubMed1983

The effect of steroids on the circulating lymphocyte population. V. Effect of prednisolone treatment on cell size and life span of the thoracic duct lymphocyte population in normal and neonatally thymectomized rats. A radioisotope study.

Using 3H-TdR isotope labelling in vivo, the effect of a high corticosteroid dose was correlated to cell size and life span of thoracic duct lymphocytes in rats. The lymphocytopenia in thoracic duct lymph during the involution phase (3 hrs after treatment) was a marked depletion of small lymphocytes, followed by a restitution to pretreatment cell levels within one day. The original lymphocyte population had a higher per cent of labelled small lymphocytes (50%) in comparison to the returning population which contained fewer (35%) small labelled cells. Label index (i.e. labelled cells of a certain cell size in percent of total cell number) was unchanged for all cell sizes during both involution and restitution phases. This unchanged label index profile as well as an earlier described unchanged cell size distribution support the hypothesis of lymphocyte trapping and redistribution as a major effect of a single prednisolone dose. The decrease of labelled small lymphocytes in the returning cell population can, however, also agree with a minor lymphocytolytic effect on circulating small lymphocytes. Our data do not support the hypothesis of two different lymphocyte populations with different life span. Neither could a redistribution of lymphocytes be found in any lymphoid tissue compartment or in femur bone marrow, during the involution phase after corticosteroid treatment.

Animals↗
PubMed1978

Influence of corticosteroids on lymphocyte recirculation.

The effect of corticosteroids on cell kinetics and cell size distribution in the circulating lymphocyte population of the steroid sensitive rat and the steroid resistant guinea pig were studied. A single high steroid dose (prednisolone) induced a rapid depression of the lymphocyte level both in normal and thymectomized animals of both species and a restitution within one day. The returning cell population showed the the same size distribution and label index profile as before involution. The main effect of a single steroid dose seems to be a "trapping" of lymphocytes with redistribution from the circulation to some tissues. The difference between the two species seems to be quantitative with a more pronounced trapping mechanism in the sensitive rat but a certain degree of lymphocytolysis cannot be excluded in this species. The steroid action seems to be on both T- and B-lymphocytes and the restitution of cell levels after acute involution is independent of an intact thymic function. The same course of events was observed in the normal rat after stress, but not in the thymectomized rat.

Adrenalectomy↗
PubMed1977

The effect of steroids on the circulating lymphocyte population. I. Changes in the thoracic duct lymphocyte population of the rat after neonatal thymectomy and prednisolone treatment.

The influence of prednisolone on the thoracic duct cells of rats was measured by the cell count in lymph at different times after a single injection and correlated with lymphocytolysis in lymphoid tissues. In both control and neonatally thymectomized animals there was a marked fall in the cell count and flow at 3 hours and the percentage reduction was greater in thymectomized animals. Restitution was rapid in both groups and pretreatment levels were regained in 17 hours. The blood mononuclear cells followed the same pattern. In animals with an intact thymus adrenalectomy causes a significant increase in thoracic duct cell counts but in neonatally thymectomized animals thoracic duct lymph is unchanged after adrenalectomy. It can be concluded that in the rat lymphocyte level in the circulating pool is thymus dependent but restoration of the circulating cell count after steroid induced involution is independent of intact thymic function. Our data support the hypothesis of lymphocyte trapping and redistribution as a major mechanism after a single cortisone dose.

Adrenalectomy↗
PubMed1977

The effect of steroids on the circulating lymphocyte population. II. Studies of the thoracic duct lymphocyte population of the guinea pig after neonatal thymectomy and prednisolone treatment.

The effect of corticosteroids on the lymphatic tissue and circulating lymphocyte pool in the guinea pig has been studied. No signs of increased lymphocytolysis was seen and only after 1--2 weeks treatment with a long acting corticosteroid was the thymic cortex reduced and thymic weight decreased. Three hours after injecting a short acting steroid a 30--40 per cent reduction in the thoracic duct cell count was seen in both control and thymectomized animals. Restitution to pretreatment cell level was completed in 17 hours. Mononuclear cells in the blood were markedly depressed up to 40 hours. The changes in the circulating lymphocyte pool in the resistant guinea pig seem to be in principle the same as in the sensitive rat but less pronounced. It can be concluded that the lymphocyte level in the circulating pool is thymus dependent but restoration of the cell count after steroid treatment is independent of intact thymic function. This data supports the hypothesis of lymphocyte trapping and redistribution after a single steroid treatment.

Animals↗