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Biomedical subjects

P M Tan

Publications and source records attributed to P M Tan.

13 recordsLinked to original sources
PubMed1997

Attitudes of the Singapore public to actions suggesting child abuse.

OBJECTIVE: The aim was to ascertain the views of the Singapore public on the acceptability of actions of an abusive nature. METHOD: In-depth interviews were carried out with 401 randomly sampled respondents in relation to a range of actions. Questions were asked concerning the acceptability of 18 actions, whether circumstances might justify eight of them, how respondents felt about reporting child abuse and whether they could recall any case they had come across. RESULTS: Respondents strongly disapproved of sexually motivated acts, and were more disapproving of physical abuse or neglect than of emotional abuse or neglect. Circumstances did affect how the less extreme actions were viewed. Respondents supported reporting child abuse, but were somewhat against mandatory reporting. They were able to recall details of a number of possible cases. CONCLUSION: We argue that definitions of child abuse should be general and not tied to specific actions, since the effects of actions may vary across cultures, and should be treated as an empirical matter. This allows a research agenda that focuses on the consequences of actions rather than issues of definition.

Adolescent↗
PubMed1990

Progressive osseous destruction as a complication of HIV-periodontitis.

A pathologic condition is described, characterized by rampant necrosis of gingival mucosa, periodontium, and related osseous structures associated with systemic infection with the human immunodeficiency virus (HIV). It is believed that this condition is an extension beyond the normal clinical course of HIV-periodontitis (HIV-P) and manifests itself in three progressive stages: (1) HIV-associated gingivitis, (2) HIV-P, and (3) an extension of HIV-P to osseous necrosis. Two cases of osseous destruction attending HIV-P are reported, one of which led to initial diagnosis of HIV infection. They represent the final stage of disease progression with localized necrosis of gingiva, periodontium, and alveolar bone.

Adult↗
PubMed1985

A sensitive SV40 viral probe assay for DNA strand breaks and their biological repair in higher cells: techniques and preliminary results.

An alternative experimental approach to the investigation of intracellular repair of DNA strand breaks by mammalian higher cells has been developed using simian virus 40 (SV40), which has no known intrinsic DNA repair capacity and possesses a minichromosome structure, as an intracellular probe. In this approach unirradiated simian or human cells are infected with irradiated virus and incubated for varying periods. Nuclei are isolated, and viral DNA is extracted and assayed for residual damage. This assay involves separation of the viral DNA by agarose gel electrophoresis into three sharply demarcated bands corresponding to DNA molecules containing a double-strand break (DSB), single-strand breaks (SSB), or no breaks. Quantitative data are obtained by a combination of DNA hybridization with 32P-labeled SV40 DNA, autoradiography, and densitometry. Various experiments have been carried out to investigate the feasibility of this approach and to establish the optimal experimental conditions for its use. These experiments indicate that there is rapid and efficient cellular uptake of SV40. independent of prior radiation dose to the virus, and that this multistep experimental procedure gives excellent recovery and quantitation of the three DNA forms when compared with more direct methods of measurement. Radiation dose-response experiments with purified extracellular SV40 virus, using this approach, are quite reproducible and give results closely comparable to those obtained with techniques in current use. Initial time-course incubation experiments with SV40 infected CV-1 monkey kidney cells indicate that this approach can demonstrate slow but extensive intracellular repair of SSB; and limited presumptive early repair of DSB, followed by later and more extensive induction of DSB.

Animals↗