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Biomedical subjects

P M Zarembski

Publications and source records attributed to P M Zarembski.

12 recordsLinked to original sources

Lead in neonates and mothers.

Blood lead was estimated in mothers post partum and infants at birth using the Jarrell-Ash 810 Atomic Absorption Spectrophotometer equipped with the Delves cup sampling system. 1665 pairs (mother and her infant) of blood samples were examined. The geometric mean for mothers and infants was 59.6 micrograms/l, SD = 25.2, and 40.7 micrograms/l, SD = 20.1, respectively. 3.5% of mothers had lower blood lead concentration than their infants. A significant correlation was observed between the blood lead concentration of infants and mothers, r = 0.8111, y = 0.6322x + 3.868 +/- 11.345. The haematocrit ratio was estimated in 1209 pairs. The geometric mean for mothers and infants was 0.3937, SD = 0.0402 and 0.5158, SD = 0.0499, respectively. No significant correlation was observed between the haematocrit ratio of infants and mothers. Similarly, no significant correlation was observed between the haematocrit ratio and blood lead concentration of mothers and infants. The examination of blood lead concentration for seasonal variation showed no significant increase in lead, although a significant drop in values was observed in spring and possible explanations offered.

Female↗

Plasma oxalic acid and calcium levels in oxalate poisoning.

Observations are reported on five cases of suicide or attempted suicide by poisoning with oxalic acid or ethylene glycol. Elevated oxalic acid levels were observed in the plasma, stomach contents, and a number of tissues. Raised oxalic acid levels in plasma were associated with reduced total and ultrafilterable calcium levels. It is suggested that the reduction in plasma total calcium level is due mainly to the deposition of calcium oxalate in the soft tissues, but inhibition of the parathyroid glands may be a contributory factor. Microscopic examination of various tissues indicated that oxalic acid is deposited in the tissues in two forms: (1) crystalline calcium oxalate dihydrate in the kidney and (2) a non-crystalline complex of calcium oxalate and lipid in liver and other tissues.

Adult↗

The fluorimetric determination of oxalic acid in blood and other biological materials.

1. Oxalic acid is separated from interfering substances by extraction with tri-n-butyl phosphate followed by co-precipitation with calcium sulphate. The precipitated oxalic acid is then reduced to glyoxylic acid, which is coupled with resorcinol to form a coloured fluorescent complex. 2. The spectrofluorometric method described is sensitive and highly specific, the minimum detectable amount of oxalic acid being 0.9mumole under the recommended conditions. 3. The concentration of oxalic acid in blood from 15 normal adults was 200-320mug./100ml. For serum the range was 135-280mug./100ml. The urinary excretion of oxalic acid by 60 normal adults on a normal diet was 9.0-28.5mg./24hr.

Blood Chemical Analysis↗