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Biomedical subjects

P Masera

Publications and source records attributed to P Masera.

At least 19 recordsLinked to original sources

Kinetically oriented manipulation of drug management in acute leukaemia.

The evaluation of the kinetic characteristics of the blast population in AL, at the outset of the disease, establishes its growth rhythm and hence the degree of invasivity. Cases with a higher 3H-Tdr labelling index also display a higher growth fraction, birth and growth rates and a shorter generation time, in other words, greater cell growth potential and invasivity. The labelling index could thus represent an immediate guide to prognosis. Particularly when combined with other prognosis factors, it can be employed to divide AL into high and low growth and invasivity forms. A distinction of this kind can thus be made the basis of differentiated therapeutic programming, whereby more or less aggressive cytocidal drug associations and doses can be administered to improve the therapeutic index and the quality of life.

Acute Disease↗

An approach to simultaneous immunofluorescent and autoradiographic analysis of human normal and neoplastic blood cells.

A technique for evaluating membrane immunofluorescence and isotope (3H-Thymidine, 3H-Uridine, 3H-Leucine) labelling in the same cell is described in detail. The possible interference of autoradiographic labelling on the fluorescent staining have been considered and found not to alter the final preparations. The technique proved valid both in normal and in neoplastic cells irrespective of their origin (peripheral blood, bone marrow, lymph node). Its possible extensions are finally discussed.

Adult↗

[T-lymphocytes in chromic lymphatic leukemia and variations of genetic activity].

By using autoradiographic methods, we have studied the actinomycin-binding in nuclei of CLL's lymphocytes cultured, in presence of PHA, at varying cellular concentrations. Chromatin activation is earlier and greater in the most concentrated culture, which thus more closely resemble normal lymphocytes' cultures. This data supports the hypothesis that T cells in CLL have normal PHA-stimulability and that their apparently delayed response is due to dilution in a predominant B cell population.

B-Lymphocytes↗