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Biomedical subjects

P Massey

Publications and source records attributed to P Massey.

At least 19 recordsLinked to original sources
PubMed1998

Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA.

The RFA1 gene encodes the large subunit of the yeast trimeric single-stranded DNA binding protein replication protein A (RPA), which is known to play a critical role in DNA replication. A Saccharomyces cerevisiae strain carrying the rfa1-44 allele displays a number of impaired recombination and repair phenotypes, all of which are suppressible by overexpression of RAD52. We demonstrate that a rad52 mutation is epistatic to the rfa1-44 mutation, placing RFA1 and RAD52 in the same genetic pathway. Furthermore, two-hybrid analysis indicates the existence of interactions between Rad52 and all three subunits of RPA. The nature of this Rad52-RPA interaction was further explored by using two different mutant alleles of rad52. Both mutations lie in the amino terminus of Rad52, a region previously defined as being responsible for its DNA binding ability (U. H. Mortenson, C. Beudixen, I. Sunjeuaric, and R. Rothstein, Proc. Natl. Acad. Sci. USA 93:10729-10734, 1996). The yeast two-hybrid system was used to monitor the protein-protein interactions of the mutant Rad52 proteins. Both of the mutant proteins are capable of self-interaction but are unable to interact with Rad51. The mutant proteins also lack the ability to interact with the large subunit of RPA, Rfa1. Interestingly, they retain their ability to interact with the medium-sized subunit, Rfa2. Given the location of the mutations in the DNA binding domain of Rad52, a model incorporating the role of DNA in the protein-protein interactions involved in the repair of DNA double-strand breaks is presented.

Alleles↗
PubMed1998

Cultural diversity in Florida nursing programs: a survey of deans and directors.

To determine how nursing programs in the state of Florida promote and integrate cultural diversity, a survey of deans and directors was conducted. Of 90 deans and directors surveyed, 46 (51%) responded. The ethnic composition of students and faculty in the respondents' programs was examined in comparison with that of the Florida population. Hispanics, Blacks, and Asians were found to be underrepresented in nursing faculty as compared with their proportion in the state population. The majority of deans and directors ranked the goal of promoting cultural diversity of moderate importance. The two most critical issues perceived by the respondents were the lack of cultural knowledge, sensitivity, and awareness, and the academic problems and educational deficits of minority students. In 50% of the programs surveyed, culture content was integrated throughout the curriculum. Other programs either integrated culture content in specific classes or offered a course in transcultural nursing. Although numerous approaches are currently being employed to recruit ethnically diverse faculty and students, the data suggest that more extensive and innovative efforts are needed to overcome existing barriers. As nursing faces the challenge of caring for a multicultural society in the 21st century, nursing leaders and organizations must collaborate to resolve the issues and problems revealed in this survey.

Cultural Diversity↗
PubMed1992

Adventurous outdoor activities: a review and a description of a new service delivery package for clients with learning difficulties who have behaviours which challenge services or society.

The purpose of this paper is to describe the need for and the creation of a new service delivery package for the client with learning difficulties whose behaviour challenges services or society, or who is a 'victim of the system', and not responding to available resources. Adventure-based courses are delivered in a context of supportive therapy which effects measurable change. There is continuing research-based evaluation of this new approach which will be reported in due course.

Camping↗
PubMed1991

Institutional loss: an examination of a bereavement reaction in 22 mental nurses losing their institution and moving into the community.

Grief has distinctive characteristics. Mental nurses working in a hospital due for closure in 15 months were assessed, and it was found that nurses with long service experienced an anticipatory bereavement in response to the expected loss of their hospital. This reaction is different from the uncomplicated grief which follows the death of a loved person, and is therefore called institutional loss. Twenty-two nurses were assessed, including 13 nurses who were still working in the hospital and nine ex-hospital nurses now working in the community. A sentence completion form was used, together with a measurement of feeling and observed emotion for each sentence. Participants had no prior knowledge about the study. Feelings identified included: initial disbelief; personal anxiety and anxiety about community care; physiological symptoms of anxiety; guilt; anger; feelings of internal loss; avoidance and searching behaviours; acknowledgement of grief; idealization; and identification phenomena. Institutional loss is characterized by: anticipatory grief; an acute stage of mourning in nurses who are physically distanced from the hospital; a refusal to accept closure as 'right'; and a strong identification with the old hospital. Institutional loss in nurses has important implications for the successful rehabilitation of large numbers of patients, and this research suggests that additional support and counselling should be offered.

Anger↗