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Biomedical subjects

P Meriläinen

Publications and source records attributed to P Meriläinen.

18 recordsLinked to original sources
PubMed2006

Effect of smoking on exhaled nitric oxide and flow-independent nitric oxide exchange parameters.

It is a well-known fact that smoking is associated with a reduction in exhaled nitric oxide (NO) levels. There is, however, limited knowledge relating to the smoking-induced changes in production or exchange of NO in different compartments of the airways. This study comprised 221 adult subjects from the European Community Respiratory Health Survey II, who were investigated in terms of their exhaled NO, lung function, immunoglobulin E sensitisation and smoking habits. The following parameters were determined using extended NO analysis: airway tissue nitric oxide concentration (Caw,NO), airway transfer factor (or diffusing capacity) for nitric oxide (Daw,NO), alveolar nitric oxide concentration (CA,NO) and fractional exhaled nitric oxide concentration at a flow rate of 50 mL x s(-1) (FeNO,0.05). Maximum total airway nitric oxide flux (J'aw,NO) was calculated from Daw,NO(Caw,NO-CA,NO). Current smokers (n = 35) exhibited lower (geometric mean) FeNO,0.05 (14.0 versus 22.8 ppb), Caw,NO (79.0 ;versus 126 ppb) and J'aw,NO (688 versus 1,153 pL x s(-1)) than never-smokers (n = 111). Ex-smokers (n = 75) were characterised by lower FeNO,0.05 (17.7 versus 22.8 ppb) and Jaw,NO (858 versus 1,153 pL x s(-1)) than never-smokers. These relationships were maintained after adjusting for potential confounders (sex, age, height, immunoglobulin E sensitisation and forced expiratory volume in one second), and, in this analysis, a negative association was found between current smoking and CA,NO. Snus (oral moist snuff) consumption (n = 21) in ex-smokers was associated with an increase in Daw,NO and a reduction in Caw,NO, after adjusting for potential confounders. Passive smoking was associated with a higher CA,NO. Using extended nitric oxide analysis, it was possible to attribute the reduction in exhaled nitric oxide levels seen in ex- and current smokers to a lower total airway nitric oxide flux in ex-smokers and reduced airway and alveolar nitric oxide concentrations in current smokers. The association between snus (oral tobacco) use and reduced nitric oxide concentrations in the airways and increased nitric oxide transfer from the airways warrants further studies.

Adult↗
PubMed2003

Administration of nitric oxide into open lung regions: delivery and monitoring.

BACKGROUND: Pulsed administration of nitric oxide has proven effective in relieving pulmonary hypertension and in improving oxygenation. With this delivery method the nitric oxide administration to low ventilated lung regions is avoided with subsequent enhancement in oxygenation. This study presents (i) pulsed administration technique for nitric oxide during artificial ventilation, (ii) evaluation of the delivery in an animal model, and (iii) validation of the delivery device in a laboratory setting. METHODS: Nitric oxide was delivered in four different pulse volumes synchronously with inspiration. The delivery was monitored with a fast responding high sensitivity nitric oxide monitor and nitric oxide uptake was calculated. Pulse delivery dose range, accuracy of the delivered dose, and stability of successive doses were analysed in a laboratory setting. RESULTS: Uptake of the delivered nitric oxide was 87-92%. Measured nitric oxide pulse concentration was 1.6-fold the delivery concentration, calculated as the ratio of nitric oxide flow to inspiration flow. Dose accuracy and stability were both 5% or 3 nmol in the validated range of 3-1000 nmol. CONCLUSION: With pulsed administration nitric oxide therapy can be directed to well-ventilated lung regions. Avoiding administration to the anatomic dead space eliminates nitric oxide exhalation effectively, which makes the method optimal for nitric oxide therapy in a rebreathing circuit. The required dose range from paediatric to adult is covered by the delivery device with a single nitric oxide gas supply.

Administration, Inhalation↗
PubMed2002

Increased nitric oxide elimination from the airways after smoking cessation.

Smokers have been found to have low exhaled nitric oxide (NO) levels. The aim of the present study was to investigate where in the respiratory system the decrease in NO occurs, and whether this decrease was affected by smoking cessation. Measurements of exhaled NO were carried out in smokers (n=20) and non-smoking control subjects (n=30). In nine of the smokers, exhaled NO was analysed 1, 2 and 4 weeks after smoking cessation. The level of exhaled NO at a flow rate of 0.1 litre/s was significantly lower in smokers (4+/-2 p.p.b.) than in non-smokers (7+/-5 p.p.b.; P=0.007). A calculation of the contributions from different areas of the lung showed that the NO flux from the airways was significantly lower (14+/-10 compared with 36+/-26 nl/min; P=0.0001) and the alveolar fraction was significantly higher (2.1+/-0.8 compared with 1.5+/-0.9 p.p.b.; P=0.006) in smokers than in non-smokers. Nine smoking subjects refrained from smoking for 4 weeks, and this resulted in increased NO flux from the airways of 28+/-17 nl/min, which was no longer significantly different from controls. In conclusion, endogenous production of NO in the airways is decreased in smokers, but can be restored to normal values by 4 weeks after cessation of smoking. Smokers have an increased alveolar fraction of NO, and this might be a diagnostic sign of lung damage. Thus NO monitoring can be used to indicate improvements when a smoker decides to stop smoking.

Adult↗
PubMed2002

Extended NO analysis applied to patients with COPD, allergic asthma and allergic rhinitis.

The recommended method to measure exhaled nitric oxide (NO) cannot reveal the source of NO production. We applied a model based on the classical Fick's first law of diffusion to partition NO in the lungs. The aim was to develop a simple and robust solution algorithm with a data quality control feature, and apply it to patients with known alterations in exhaled NO. Subjects with allergic rhinitis, allergic asthma, chronic obstructive pulmonary disease (COPD) smokers and controls were investigated. NO was measured at three expiratory flow rates. An iteration method was developed to partition NO. The airway tissue content of NO was increased in asthma, 144 +/- 80 ppb (P = 0.04) and decreased in smokers, 56 +/- 36 ppb (P = 0.02). There was no difference between subjects with rhinitis, 98 +/- 40 ppb and controls, 98 +/- 44 ppb. The airway transfer rate was increased in allergic asthma and allergic rhinitis, 12 +/- 4 vs. 12 +/- 5 ml sec(-1), compared to controls, 8 +/- 2 ml sec(-1) (P < 0.001). The alveolar levels were no different from controls, 2 +/- 1 ppb. In COPD the alveolar levels were increased, 4 +/- 2 ppb (P < 0.001). Extended NO analysis reveals from where in the respiratory system NO is generated. Hence, this new test can be added to the tools the physician has for the diagnosis and treatment of patients with respiratory disorders.

Administration, Inhalation↗
PubMed2002

Effect of different pulses of nitric oxide on venous admixture in the anaesthetized horse.

BACKGROUND: Dependent atelectatic lung areas open towards the end of inspiration when the lung opening pressure increases, and recollapse during expiration. We hypothesized that inhaled nitric oxide (NO) counteracts hypoxic vasoconstriction in these collapsing lung areas, resulting in increased pulmonary shunt perfusion. METHODS: We administered NO as a pulse and varied the pulse timing during inspiration in equine anaesthesia, where atelectasis develops regularly. Six spontaneously breathing standard breed trotters were studied under isoflurane anaesthesia in lateral recumbency. NO pulsed into the first 30% of inspiration (group NOp1) was assumed to affect open lung areas. To cover more open lung areas NO was then pulsed into the first 60% of inspiration (group NOp2). In a third group, administration between 50 and 80% of inspiration was aimed at the intermittently opening lung areas (group NOp3). RESULTS: With NOp1, venous admixture decreased by 8 (2)% (mean (SEM), P=0.045) and with NOp2 by 10 (1)% (P=0.01). With NOp3, venous admixture reduction was insignificant. CONCLUSIONS: Pulsed administration of NO in early inspiration is optimal in reducing right to left vascular shunt in atelectatic equine lung. This reduction is positively correlated with the magnitude of the initial shunt. With administration in early inspiration, NO is mostly taken up by the lung. This prevents NO accumulation and NO2 formation in rebreathing circuits. These findings may be important in humans when atelectasis occurs increasingly with overweight and age during anaesthesia, but also in postoperative intensive care and in ARDS.

Anesthesia, Inhalation↗
PubMed2001

Inhaled mannitol shifts exhaled nitric oxide in opposite directions in asthmatics and healthy subjects.

We investigated if healthy subjects could release NO upon hyperosmolar challenge as a defence mechanism, and whether asthmatics with atopy showed an altered response. A plot of NO output versus flow rate was used to calculate the alveolar level and the NO-flux from the airways. The asthmatics had a higher NO output and this was due to an increased NO-flux from the airways, 86+/-30 nl min(-1) compared with control 21+/-2 nl min(-1) (P<0.05). The alveolar NO levels showed no difference. In response to a dry powder of mannitol the exhaled NO concentration decreased in asthmatics by 37+/-7%, but increased in the control by 9+/-4% (P<0.001). The FEV(1.0) decreased 13+/-2% and airway conductance 42+/-7% in asthmatics and in the controls 2+/-1% and 0+/-7%, respectively (P<0.001). We conclude that asthmatics have an altered response to mannitol challenge in regards to exhaled NO. This may result from down regulation of constitutive NO production as a result of high levels of NO flux from the airways.

Administration, Inhalation↗
PubMed2001

Informal caregivers' participation in elderly-patient care: an interrupted time-series study.

This study is an evaluation of a project in Finland that aimed to promote the participation of informal caregivers in the hospital care of elderly patients. The staff who worked in the wards that were studied built up activation programmes for informal caregivers, and changed ward policies to encourage caregivers' participation. To explore changes in caregivers' participation in the study and the control wards, non-equivalent control groups were used. During periods of two months in 1991, 1992 and 1993, the data were collected from informal caregivers (N = 369) using a structured questionnaire. The results indicated that total participation of informal caregivers in daily activities increased in long-term care settings, but not in the university hospital. However, a small increase in informal caregivers' participation in some daily activities of elderly patients took place in the study wards of each setting. The findings and implications for nursing are discussed.

Activities of Daily Living↗
PubMed2000

Theoretical and experimental comparison of constant inspired concentration and pulsed delivery in NO therapy.

OBJECTIVE: Inhaled NO therapy of artificially ventilated patients has been established as being based on constant inspired concentration of NO. In this study a new volumetrically controlled pulsed NO delivery mode is compared with the established concentration-based concept. DESIGN: To evaluate the relationship between NO delivery parameters, alveolar NO fraction, and patient uptake, a mathematical lung model was created where NO delivery can be simulated in varying ventilator settings, delivery modes, and lung properties. This model and the efficacy of pulsed delivery in inducing pulmonary capillary vasodilatation were examined experimentally. SETTING: Animal laboratory, Department of Medical Sciences, Clinical Physiology. SUBJECTS: The experimental study was performed with nine pigs of mixed breed weighing 25-35 kg. INTERVENTIONS: The pigs were anaesthetised and artificially ventilated. Pulmonary vasoconstriction was induced by hypoxia. NO was delivered periodically in the various delivery modes. MEASUREMENTS AND RESULTS: In simulation, in all delivery modes the NO uptake was found to be dependent on the ventilator settings and the volume of the dead space. Measured from pulmonary artery pressure, the pulsed delivery was as effective in reducing the induced pulmonary vasoconstriction as the constant inspired concentration delivery. The amount of NO that could reduce the vasoconstriction back to baseline was 105 nmol x min(-1). By delivering in the early part of the inspiration, ambient contamination by the exhaust gas is avoided. The expired NO values obtained in the simulation and the experiments were equal. Based on the simulation, the alveolar NO fraction and the NO uptake depend on the ventilator settings and the dead space in both volumetric- and concentration-based delivery. CONCLUSIONS: With pulsed delivery, a therapeutic effect comparable to constant inspired concentration delivery is achieved, NO gas is used more effectively, and environmental exhausts are reduced. The theoretical model shows that the NO delivery does not predict alveolar NO fraction and the NO uptake. However, it still remains an open question if the online measurement of these parameters would provide useful information, having added value in predicting and controlling the efficacy of the NO treatment.

Animals↗
PubMed2000

Exhaled nitric oxide partitioned into alveolar, lower airways and nasal contributions.

During the last year exhaled nitric oxide (NO) has been proposed as a marker of airway inflammation. More knowledge of the production and transfer of this molecule are needed in order for NO analysis to become a clinical tool. This was the aim of the study. Exhaled NO values from multiple flow rates were used to model alveolar NO, transfer rate and tissue concentration of NO in the airways. Three flows rates, 0.005, 0.1 and 0.51 sec(-1) were found to be optimal. The NO transfer rate of the airways was 9 +/- 2 ml sec(-1), the tissue source was 75 +/- 28 ppb and the alveolar fraction of NO was 2 +/- 1 ppb in 10 healthy subjects (mean +/- CI95%). In conclusion, we have shown that it is possible to get more information about the distribution of NO in the lungs and the airways than only a single value from one expiratory flow rate can give. Further studies will reveal if this airway modelling can be useful in disease of the respiratory system.

Biomarkers↗
PubMed1998

Error in measurement of oxygen and carbon dioxide concentrations by the DeltatracII metabolic monitor in the presence of desflurane.

In experiments in dogs on the metabolic effects of inhalation anaesthetics, we noticed that in the presence of desflurane, oxygen uptake (VO2) measured with the Deltatracll metabolic monitor seemingly increased whereas it decreased when determined independently by the Fick principle. This difference remained even after correction for changes in gas concentration on addition of an inhalation anaesthetic. Therefore, we suspected that desflurane interferes with the measurement of gas concentrations. Using different precision gases, we found that desflurane disturbed both the paramagnetic oxygen sensor and the infrared carbon dioxide detector so that the measured oxygen (when FIO2 was > 0.21) and carbon dioxide concentrations were greater than expected. These errors multiply in the computing process of oxygen uptake by the DeltatracII. When the DeltatracII is to be used during inhalation anaesthesia, its results should be corrected for the presence of an anaesthetic gas. More importantly, corrections must also be made for measurement errors of the oxygen and carbon dioxide sensors, unless the device has been equipped with a modified (nickel membrane) oxygen sensor insensitive to the presence of volatile agents.

Anesthetics, Inhalation↗
PubMed1998

[Sleep disturbances affecting hospital patients].

The purpose of this article is to describe patients' sleep in hospital. The article is based on a study of patients' sleep in a hospital's medical or surgical ward and to clarify the disturbance factors relating to their sleep. The patients of the Central Hospital of Northern Carelia's two medical and two surgical wards took part in the research. The data were collected by a structured questionnaire and a follow-up questionnaire in May-June 1996. Of the replies of 181 patients, the results of 177 were taken to the final analysis. The data were analysed by statistical methods. The data and the results are presented in frequency and percent distributions, and the background variables of those patients sleeping well and those sleeping poorly are analysed and compared. The results indicated that 65% of the researched patients slept badly in the hospital. Environmental factors were found to be related to most of the patients' sleep disturbances: 80% of them regarded those factors as the cause for their disturbed sleep. Other patients, noise, and the nurses' work were regarded as the most disturbing of the environmental factors. The internal factors had disturbed patients' sleep in the hospital less than the environmental factors. Pain was regarded as the most sleep disturbing internal factor: over half of the researched patients felt disturbed by it. During their stay in the hospital the patients experienced more positive (trust, contentment, safety) than negative feelings (fear, anxiety, depression, distrust), and those who experienced negative feelings had more difficulties in sleeping.

Adult↗
PubMed1998

[Methods used in the hospital for patients' sleep improvement].

The purpose of this article is to describe methods used in the hospital for sleep improvement. The article is based on a study of patients' sleep in a Finnish hospital's medical or surgical ward. The patients of the Central Hospital of Northern Carelia's two medical and two surgical wards took part in the research. The data were collected by a structured questionnaire in May-June 1996. Of the replies of 181 patients, the results of 177 were taken to the final analysis. The data were analysed by statistical methods. The data and the results are presented in frequency and percent distributions. The most common method used by the patients to improve their sleep was to choose a good sleeping position. Other methods, which were often used, were reading, watching TV, having a snack, taking a warm shower or praying. The nurses were versatile in using the available methods of nursing for helping the patients to sleep. They informed the patients about their illnesses, nursing regime and the forthcoming operations, consoling and giving support to them. The nurses also organised sleep-supporting environment and gave painkillers and sleeping pills. About 60% of the researched patients were of the opinion, that sleeping tablets were prescribed too often. The patients themselves regarded the information and knowledge about their own treatment as the best way to improve their sleep.

Hospital Units↗
PubMed1996

Quality of elderly-patient care: an interrupted time series study.

This study is part of a project that aimed to increase the participation of informal caregivers in the hospital care for elderly patients. The staff who worked in the wards that were studied created activity programmes for caregivers and changed their ward policies to encourage the participation of the caregivers. In addition, quality assurance programmes were developed and primary nursing was introduced to ensure individualized, need-based and family centered care. In order to explore the changes in perceived quality of care, and to test the differences in care quality between the study and the control wards (i.e. two wards in a university hospital, two wards in a geriatric unit and two wards of a nursing home), the interrupted time series with a non-equivalent control group time series design was used. During periods of 2 months in 1991, 1992 and 1993 the data were collected from elderly patients (n = 97) and their informal caregivers (n = 369) using a structured questionnaire based on need theories. The results indicated that the quality of care was improved, especially in the study wards of the university hospital and of the nursing home. This care quality measure was found to be a useful tool for getting feedback from patients and their caregivers concerning care given. Aspects of nursing practice that needed to be improved were identified (i.e. poor communication between the patient, their caregiver and staff, and lack of support to the patient and caregiver by staff).

Aged↗
PubMed1994

[Modification of Colaizzi's phenomenological method; a study concerning quality care].

The aim of this article is to describe Colaizzi's (1978) phenomenological method using as an example a study of good care. The purpose of phenomenological method is to uncover the genuine experience of the phenomenon under investigation; Colaizzi's method consists of seven steps. First informants' descriptions of the experiences are read in order to acquire a sense of the whole. After that significant statements are extracted. Meanings are formulated from the significant statements. Formulated meanings are organised into themes. Themes are integrated into an exhaustive description. The essential structure of the phenomenon is formulated. And finally for validation the informants will evaluate the result of the analysis, if it means the same as their original experiences were. The modification of Colaizzi's method was used in order to conceptualize good care based on the experiences of a population of ex-patients.

Clinical Nursing Research↗
PubMed1993

A novel sensor for routine continuous spirometry of intubated patients.

Measurement of gas flow and airway pressure at the Y-piece of an endotracheal tube provides valuable information about airway integrity and basic pulmonary function. We describe the working principle and design of the D-lite sensor (Datex Division, Instrumentarium Corporation, Helsinki, Finland), which, in a single, lightweight adapter piece, encloses flow, airway pressure, and sidestream gas measurement. The main emphasis in the design of this instrument was on reliable and accurate operation during continuous monitoring with exposure to humidity and mucus. Therefore, a robust flow-restrictor element with a pick-off arrangement resembling a Pitot tube was employed. This arrangement has nonlinear characteristics with potential difficulties in the measurement of small flows. However, these inherent drawbacks, together with compensations required because of varying gas fractions and pressure, can be handled by sophisticated computer algorithms at the host-monitor end (Capnomac Ultima [Datex Division, Instrumentarium Corporation, Helsinki, Finland]). Validation methods with main results and a brief review of applications are given.

Airway Resistance↗
PubMed1991

[Practice experience as a resource--the Delphi method in nursing research].

Delphi method in nursing research is unusual but worthy of attention. The foundation of the method is experience-based knowledge and interaction of experts in successive rounds. Usually delphi method does not produce objective, exact facts. Delphi method is applicable to identifying and clarifying new issues and assumptions and producing well-grounded evaluations--often important in young disciplines. However, critical attitude is needed regarding methodological decisions as well as implications.

Delphi Technique↗