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Biomedical subjects

P Messner

Publications and source records attributed to P Messner.

At least 109 records · Page 6Linked to original sources
PubMed1985

[Distribution of lactate dehydrogenase isoenzymes in human myocardium obtained from endomyocardial biopsies].

The distribution of lactate dehydrogenase and its isoenzymes in myocardial tissue obtained by biopsies from within the right ventricle can be studied by electrophoresis in a similar manner to that for making serum determinations. Results are comparable to radioimmunologic dosing (Peters) and microelectrophoresis (Schultheiss) and complete the findings obtained from the histological study of biopsied fragments.

Biopsy↗
PubMed1985

Monoclonal ELISA for the determination of BNYV-virus.

Monoclonal antibodies have been developed against the BNYV-virus (beet-necrotic-yellow-vein) which causes the rhizomania disease in sugar beet (Beta vulgaris). The B103 hybridoma produced predominantly IgG2a at greater than 100 micrograms/ml. IgM-production could not, however, be ruled out. In a polyclonal-monoclonal indirect sandwich ELISA, the B103-antibody bound to BNYVV of Austrian, German, French and Bulgarian origin at a sensitivity of 1 ng/ml or less. Comparison of sugar beet samples in ELISA and immuno-electronmicroscopy showed good correlation, demonstrating the good performance of B103 antibody for broad application in BNYVV diagnosis.

Antibodies, Monoclonal↗
PubMed1981

Effect of the growth environment on cell-envelope components of Escherichia coli in relation to sensitivity to human serum.

Three smooth strains of urinary Escherichia coli were grown in a chemostat under carbon-limited (C-lim) and magnesium-limited (Mg-lim) conditions over a range of dilution rates (D). Strain LP1674 was resistant to human serum under C-lim but became sensitive when grown under Mg-lim, the degree of sensitivity increasing as D increased. The transition to serum sensitivity was accompanied by loss of ability to produce extractable K1 antigen and a reduction in the amount of a 46k envelope polypeptide. C-lim cells of strain LP729 exhibited a delayed sensitive response to serum, the degree of lag in serum killing becoming less pronounced with increasing values of D; Mg-lim cells were more sensitive with little or no lag in serum killing. The degree of lag appeared to be directly related to the amount of the O side-chain sugar mannose associated with the lipopolysaccharide. C-lim and Mg-lim cultures of E. coli strain LP1395 were resistant to serum except when growing at near maximal rates. Although C-lim cultures contained more acidic polysaccharide than Mg-lim cells, transition to serum sensitivity did not appear to be related to exopolysaccharide production. Rapidly growing cells of strain LP1395 did, however, have lower lipopolysaccharide 0 side-chain sugar:core-sugar ratios than more slowly growing cells. With all three strains, changes in dilution rate and in the nature of the limiting nutrient were accompanied by changes in envelope protein composition. This study demonstrates that many cell-surface changes occur in response to alterations in the growth environment and some of these may be correlated with changes in sensitivity to serum.

Bacterial Proteins↗