PubMed Health⌕ Search

Biomedical subjects

P Moss

Publications and source records attributed to P Moss.

47 records · Page 3Linked to original sources
PubMed1984

The role of protein phosphorylation at tyrosine in transformation and mitogenesis.

In cells transformed by avian sarcoma viruses or stimulated by growth factors, certain polypeptides become phosphorylated at tyrosine residues. It is not known if these cellular polypeptides are phosphorylated directly by the tyrosine-kinase activities which are associated with the viral transforming proteins and with growth factor receptors. It is also not clear if phosphorylation of these polypeptides is required for viral transformation or the response to growth factors. We describe here some observations which bear on these questions and discuss possible future approaches.

Alpharetrovirus↗
PubMed1984

Cellular localization of the transforming protein of wild-type and temperature-sensitive Fujinami sarcoma virus.

Fujinami sarcoma virus (FSV) encodes a 140,000-dalton transforming protein, P140, which contains gag- and fps-specific sequences. The cellular localization of this protein was examined by fractionation of [35S]methionine-labeled, FSV-infected chicken embryo fibroblasts. In homogenates of cells infected by wild-type, temperature-resistant FSV prepared in either hypotonic or isotonic buffer, 60 to 80% of the P140 was particulate. Isopycnic separation on discontinuous sucrose gradients indicated that the majority of the particulate P140 was present in a light membrane fraction enriched for plasma membranes. Much of the particulate P140 could be solubilized by the addition of 0.6 M salt to a postnuclear supernatant, suggesting that P140 is not an integral membrane protein. Particulate P140 may be associated with membranes either directly as a peripheral membrane protein or indirectly via cytoskeletal elements. In cells infected by mutants of FSV temperature sensitive for cellular transformation, most of the P140 is particulate at the permissive temperature, whereas most is soluble at the nonpermissive temperature; this change in distribution is not a secondary consequence of the change in cellular phenotype, since it also occurs in nonconditionally transformed cells doubly infected with temperature-sensitive FSV and wild-type Rous sarcoma virus. The movement of P140 from the particulate to the soluble fraction occurs rapidly when cells infected by temperature-sensitive FSV are shifted from the permissive to the nonpermissive temperature. Furthermore, P140 moves from the soluble to the particulate fraction, although somewhat more slowly, when cells are shifted from the nonpermissive to the permissive temperature. These observations suggest that the association of P140 with plasma membranes or the cytoskeleton may play a role in transformation by FSV.

Animals↗
PubMed1983

Membrane association of a 36,000-dalton substrate for tyrosine phosphorylation in chicken embryo fibroblasts transformed by avian sarcoma viruses.

A cellular protein of 36,000 daltons becomes phosphorylated at tyrosine in chicken embryo fibroblasts transformed with avian sarcoma viruses. We have used cellular fractionation and immunofluorescence to locate the 36-kdalton protein in virus-transformed and uninfected chicken fibroblasts. The 36-kdalton protein in transformed cells fractionated mainly with high-speed particulate material, and in density gradient separations, the 36-kdalton protein was found in association with light density membranes together with most of the plasma membrane marker. Increasing the concentration of salt or adding ion chelators solubilized some of the 36-kdalton protein that otherwise was pelletable with high g forces. Based on these data, we conclude that this protein is peripherally or indirectly attached to light density membranes, including plasma membranes. Indirect immunofluorescent staining of the 36-kdalton protein in fixed cells revealed that it was located inside the cell in an extensive reticulum apposed to surface membranes. The same pattern of staining was found in both uninfected and virus-transformed cells. Pretreatment of cells with nonionic detergents before fixation altered or abolished 36-kdalton staining. The 36-kdalton protein appeared to be excluded from regions of the cells where actin cables were present. The pattern of staining observed with the anti-36-kdalton antibody was similar, but not identical, to that observed with antiserum against nonerythroid spectrin. Thus, the data obtained by biochemical fractionation and by immunofluorescent staining indicate that the 36-kdalton protein is found in a reticulum at the inner surface of the plasma membrane, possibly in association with cytoskeletal proteins.

Animals↗
PubMed1977

Mental distress in mothers of pre-school children in inner London.

The level of mental 'distress' was assessed for a sample of mothers with pre-school children in Inner London. Fifty-two per cent of mothers had a moderate or severe distress problem in the 12 months prior to interview. Variables correlating with distress were identified and replicated in a second sample. Social class was not found to be related, and the question of the general relationship between social class and distress is considered.

Child, Preschool↗
PubMed1971

Bacterial metabolism of 4-chlorophenoxyacetate.

1. A pseudomonad capable of utilizing 4-chlorophenoxyacetate (CPA) as sole source of organic carbon was isolated from soil. 2. The organism was grown in liquid culture and the following compounds were isolated and identified in culture extracts: 4-chloro-2-hydroxyphenoxyacetate, 4-chlorocatechol, beta-chloromuconate probably the cis-trans isomer and gamma-carboxymethylene-Delta(alphabeta)-butenolide. 3. Cells grown on 4-chlorophenoxyacetate were able to metabolize 4-chloro-2-hydroxyphenoxyacetate, 4-chlorocatechol and gamma-carboxymethylene-Delta(alphabeta)-butenolide without a lag period. They were not adapted to 4-chlorophenol, or to either culture isolated or synthetic beta-chloromuconate, possibly because of stereospecificity towards the cis-cis isomer. 4. On the basis of isolation and induction evidence, the following metabolic pathway is proposed for the breakdown of 4-chlorophenoxyacetate by this organism: 4-chlorophenoxyacetate --> 4-chloro-2-hydroxyphenoxyacetate --> 4-chlorocatechol --> cis-cis-beta-chloromuconate --> gamma-carboxymethylene-Delta(alphabeta)-butenolide --> maleylacetate and fumarylacetate --> fumarate and acetate.

Acetates↗
PubMedDate not supplied

The hospital inpatient stay: the experience of first-time parents.

The experience of hospital inpatient stays at childbirth was studied in a longitudinal study of the experiences of 96 first-time parents during the transition to parenthood. For women, the prospect of birth was a major worry during pregnancy. Negative expectations proved in general to be justified by events, with many women reporting predominantly bad memories of the experience. Satisfaction with the hospital inpatient stay is also considered: a major source of dissatisfaction was inadequate help with breast feeding, which produced many difficulties. Middle class mothers were in general more dissatisfied with their hospital stay, as were women who had Caesarean births. Relationships were found between antenatal class attendances and the likelihood of a normal delivery and reported pain during childbirth. The role and experience of fathers was also considered. Most expected to be at the birth from an early stage in pregnancy; more than two-thirds were present for the delivery and half or more at the labour. Their experience at this time was more positive than that of their partners. But during the hospital stay, this role was much more marginal. The results are discussed in terms of their implications for antenatal preparation, breast feeding and paternal involvement.

Adult↗