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Biomedical subjects

P N Sharaev

Publications and source records attributed to P N Sharaev.

At least 19 recordsLinked to original sources
PubMed2006

[Determination of serum fucosidase activities].

A method has been developed for simultaneous determination of the activity of alpha-L-fucosidase (EC 3.2.1.51) and the beta-D-fucosidase activity induced by beta-D-galactosidase (IV, EC 3.2.1.23) in the serum. The paper presents data on changes in these enzymatic activities in the serum in an experiment, in acute hematogenous osteomyelitis and recurrent laryngotracheitis in children in pregnant females with gestosis and placental insufficiency.

Adult↗
PubMed2006

[Metabolism of the main substance of the connective tissue in chronic obstructive pulmonary disease].

AIM: To reveal metabolic disturbances of connective tissue main substance in chronic obstructive pulmonary disease (COPD) and to assess their informative value in determination of treatment efficacy. MATERIAL AND METHODS: Peripheral blood of 130 COPD patients was studied for total glycosaminoglycanes, hyaluronidase activity, sialic acids fractions (free, oligobound and proteinbound), fucose fractions (oligobound and proteinbound) and activity of sialidase. RESULTS: The levels of total glycosaminoglycanes, sialo- and fucose-containing compounds in the blood change with COPD severity and phase of the disease. CONCLUSION: The data obtained may be used to assess activity of respiratory inflammation and efficacy of the treatment.

Connective Tissue↗
PubMed2006

[Detection of serum fucosidase activity].

A procedure has been developed for simultaneous determination of the activity of a-L-fucosidase (EC 3.2.1.5 1) and the beta-D-fucosidase activity caused by beta-D-galactosidase (EC 3.2.1.23, Form IV) in serum. The paper presents data on changes in the above serum enzymatic activities in an experiment, in children with recurrent laryngotracheitis and in pregnant females with gestosis and placental insufficiency.

Acute Disease↗
PubMed2005

[Characteristics of collagen metabolism in duodenal ulcer].

We studied the content of collagen metabolites in patients with duodenal ulcer (blood plasma, urea, and samples of the duodenal mucous tunic and mouth fluid). We also defined the role of an affected collagen metabolism in the formation and cicatrization of duodenal ulcer; finally, we ascertained that the nature of the detected changes in different biological substrate is unidirectional.

Adult↗
PubMed2004

[Detection of N-acetyl-beta-D-hexosaminidase in urine].

A new modification was designed to determine the activity of N-acetyl-beta-D-hexosaminidase (NAH) in biological fluids. The properties of enzyme molecules to adsorb themselves on chitin at pH 4.5 were made use of within the case study. The mean NAH activity, in urine of virtually healthy children and adults, was shown to be equal, within a span time of 1 h, to 12.8 and 22.3 mumole of formed 4-nitrophenol/per 1 mmole of creatinine. The above mean NAH parameter was, in the amniotic fluid of pregnant women, 696 nmole of 4-nitrophenol/per 1 ml in 1 h. The NAH activity was studied in renal pathologies and in delayed intrauterine development of the fetus.

Adolescent↗
PubMed2001

[The method for detection of urinary lysozyme].

A method for lysozyme measurement in the urine is developed, based on adsorption of lysozyme molecules on the surface of chitin. The urine of healthy children, men, and women contains 1.76, 1.51, and 1.69 mg/liter lysozyme, respectively. Urinary lysozyme values in some diseases are presented.

Biological Assay↗
PubMed1997

[Method of measuring non-protein bound fucose].

A method for measuring fucose not bound to proteins in the serum is developed. The level of this enzyme is shown to increase with age. It is increased in adult patients with myeloma, liver cirrhosis, and rheumatic fever and in children with exacerbations of chronic osteomyelitis and gastroduodenitis.

Adolescent↗
PubMed1991

[Indices of hexosamine-containing biopolymers in experimental tooth replantation].

The experimental findings showed that the content of hexosamine-containing biopolymers, in periodontal tissue increased by 38.23 and 124% conformably over 14 days after replantation of 4 teeth of the dogs. The indices normalized in 28 days of the experiment. Investigated biochemical parameters in the root of replantation tooth, in osseus tissues of alveolus 2nd blood. Changed slightly in dynamics.

Alveolar Process↗
PubMed1990

[Determination of the oligobiopolymerized sialic acids in the blood].

A method for measurement of the blood serum (plasma) oligobiopolymerized sialic acids (OSA) has been developed, making use of the known procedures for measuring these amino sugars. Blood OSA levels of normal subjects and intact rats have made up 59.6 +/- 2.3 and 79.0 +/- 3.4 mg/l, respectively. OSA levels were shown to rise in immobilization stress (by 36 percent), aseptic granulomas (by 28 percent), and active phase of rheumatic fever (by 128 percent). The method is recommended for clinical diagnostic studies as an additional diagnostic test.

Animals↗
PubMed1990

[The determination of free and bound oxyproline in urine].

A method of measuring urinary free, peptide- and protein-bound hydroxyproline is suggested. Adults were found to excrete 18 mumol of free, 155 mumol of peptide-bound, and 8 mumol of protein-bound hydroxyproline daily. In children daily urinary levels of free and peptide-bound hydroxyproline were higher. Data are presented on changes in various hydroxyproline forms in pneumonia, rheumatic fever, and hemorrhagic fever with the renal syndrome.

Adult↗
PubMed1990

[Predicting the course of wound healing after appendectomy using the hydroxyproline test].

A test for free hydroxyproline in blood plasma, urine, and wound exudate was conducted in a group of children who were operated on for acute catarrhal and destructive forms of appendicitis in an uncomplicated or complicated post-operative wound process. The changes in the concentration of the biochemical index were most regular in the wound exudate. Free hydroxyproline increased after the operation, reached highest values in 48 hours, and then reduced coming close to the control values 5-6 days after. In wound complications it increased three- to four-fold as compared to the control values, which could serve as an informative test in predicting the course of the postoperative wound process in children.

Acute Disease↗
PubMed1988

[Regulation of glucosamine synthetase activity by cholesterol and hydrocortisone].

The effects of cholesterol and hydrocortisone (cortisol) on the activity of purified glucosamine synthetase from rat liver was studied in vitro. It was found that the enzyme activity is increased by cholesterol and inhibited by hydrocortisone. These steroids block the allosteric effect of vitamin K1 on the enzyme. There is evidence testifying to the allosteric type of cholesterol and hydrocortisone effects on glucosamine synthetase.

Animals↗