Biomedical subjects
P Nakane
Publications and source records attributed to P Nakane.
Tumours of the pituitary gland.
Explore the source record for details and available documents.
Anaerobic energy metabolism in brain tumors.
Explore the source record for details and available documents.
Effect of ionizing radiation on nuclear energy transduction in normal and neoplastic glia. A quantitative cytochemical investigation.
Explore the source record for details and available documents.
Isolation and characterization of glioblastoma nuclei and chromosomes in the lyophilized state.
Explore the source record for details and available documents.
Bulk isolation in nonaqueous media of nuclei from lyophilized cells.
Intact lyophilized nuclei are obtainable from a variety of tissues, either in situ or in culture, by freezing at -156 degrees C, drying at -25 degrees C, and mechanical disassociation in glycerol at 2 degrees C. Centrifugal separation of nuclei is accomplished in an 85 : 15 by volume mixture of glycerol and 3-chloro-1,2 propanediol at 2 degrees C. The method gives homogeneous nuclear preparations in high yield with preservation of labile and water-soluble constituents.