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Biomedical subjects

P Nevers

Publications and source records attributed to P Nevers.

9 recordsLinked to original sources

Biology as technology.

In this paper we have emphasised the technological dimension of the biosciences, especially the biosciences of our time. As a straight forward fact this dimension is less controversial than the question of its relevance is. We have argued that the existence of this dimension is a consequence of the empirical character of the biosciences (as well as of the sciences in general). Knowledge of nature can be achieved only if the subject of knowledge is able to gain access to natural phenomena, and technological means have become increasingly necessary for achieving this. The more that is already known, the greater the technological investment required to produce new knowledge. Therefore there is an interrelationship between the advancement of knowledge and the amount and complexity of the research technology required. It would be short sighted to conclude from this observation that human imagination and inventiveness have become superfluous. Nevertheless the growing preponderance of technological means for progress in science is undeniable. A particularly important insight is that this process not only involves a quantitative increase in technology within science. Our main hypothesis is that there is a close relationship between the type of means used, the type of subject required for performing research, the kinds of objects investigated in science, and the nature of the results that are generated. We have tried to illustrate this by distinguishing between three different types of bioscience: (a) a descriptive type, (b) an experimental type, and (c) and an industrial type. Without pretending this provides a universal key to the history of the biosciences and to understanding of the way science works today, we hope that such a distinction may open up new avenues of thought. We hope that this approach will provide us with a more realistic picture of science. The propositional view reduces science to its theoretical results, in particular to the theories emanating from basic research. Science is seen as a special kind of philosophy; its central aim is to provide us with a 'true' view of the world. Thus the social problems which result from scientific inquiry and from the application of its results seem to be something external to the very essence of science and thus only of secondary importance. We believe, therefore, that this picture of science is both theoretically unsatisfactory and socially misleading. An appropriate account of science cannot ignore the fact that basic research represents only a very small part of the science system of our time. It cannot neglect the fact that science today is steeped with technology and to a great extent also industrialised. And it cannot treat the social problems of science as something merely 'external'. To view science as an activity in the sense outlined in this paper permits one to integrate these different aspects into one coherent and realistic picture. 1. It is obvious that technology plays a crucial role in determining the social reality of science. This is mainly because of the high costs of contemporary research technology that science can no longer be performed by gentleman scientists like Alexander von Humboldt or Charles Darwin, who not only financed their own living but the cost of their own research too. Science today is a profession, and in many cases it can be performed only in large groups or institutions financed by the state or by private companies. At the same time this means that it is more and more dependent upon decisions made outside science. Scientific activities have assumed the economic form of wage labour. The internal structure of science is characterised by a division of labour and hierarchical forms of decision making. 2. From a more traditional point this may seem to be a development 'external' to science, without any relevance for its 'essence'. The aim of science, it may be said, is to discover the truth about the external world, and the ways of reaching it a

Biological Science Disciplines↗

Transposition in plants: a molecular model.

A molecular model for transposition of plant transposable elements is described. This process may occur via excision and re-integration of the element. Excision generates DNA sequence diversity which suggests the participation of DNA repair enzymes in the healing of the donor molecule.

DNA Transposable Elements↗

Transposable genetic elements as agents of gene instability and chromosomal rearrangements.

Transposable genetic elements in prokaryotes and eukaryotes, when inserted at a given locus, can control expression of the locus and cause large scale rearrangements of adjacent DNA sequences. Striking similarities in genetic behaviour between the two groups of elements have led to the proposal of a molecular model of eukaryotic controlling elements, and to suggestions about the part such elements may play in evolution and differentiation.

Base Sequence↗

Escherichia coli mutants uvr D and uvr E deficient in gene conversion of lambda-heteroduplexes.

Calcium-treated cells of E. coli K-12 C600 were transfected with lambda-heteroduplex DNA carrying the marker cIts857 in one strand and wildtype in the other. In single burst analyses of the phage progeny, 72-79% of the bursts were "pure" bursts containing either exclusively wildtype phage or exclusively mutant phage, indicating that conversion of the cIts857/+ mismatch to a homoduplex structure prior to replication occurred with this frequency. The r-strand1 appears to be "preferred", since pure bursts of progeny with the r-strand genotype were almost twice as frequent as those with the l-strand genotype. Examination of the conversion frequency of a number of rec and uvr E. coli mutants showed that the mutants uvr D and UVR E are deficient in mismatch repair. Conversion is reduced in the former by a factor of 2 and in the latter by a factor of 3.

Calcium↗

Precise and nearly-precise excision of the symmetrical inverted repeats of Tn5; common features of recA-independent deletion events in Escherichia coli.

The transposon Tn5 contains a unique central region bordered by 1.5-kb inverted repeats. The in vitro deletion of the centre of Tn5, with a restriction endonuclease (XhoI) which cuts within the inverted repeats leads to the production of a palindrome on subsequent ligation. This palindromic region is unstable on subsequent transformation into Escherichia coli (Collins, 1981). Precise excision of the Tn5 region plus one copy of the bracketing 9-bp direct repeat occurred in about one-third of the transformants. The rest of the transformants contain only remnants of the inverted repeat. Sequence analysis indicated that deletion had occurred between short direct repeats. The precise excision of these "nearly precise" excision products continued with high frequency and was found to be affected by mutations that interfere with the normal precise excision of transposons. In a recB, sbcB host precise excision was markedly reduced. A common mechanism is proposed for all recA-independent deletions occurring in E. coli.

Bacterial Proteins↗