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Biomedical subjects

P O Lind

Publications and source records attributed to P O Lind.

18 recordsLinked to original sources
PubMed2000

Extraradicular infection: a methodological study.

Whether bacteria live or die in periapical lesions of endodontic origin is debated. Sampling of periapical bacteria is difficult due to possible contamination from the indigenous microflora. The aim of this study was to examine whether bacteria were present in periapical lesions of asymptomatic teeth before sampling or were transferred there during sampling. Thirty patients with root-filled teeth and periapical radiolucencies were divided into two groups, each containing 15 patients. In Group 1, a marginal incision was made to explore the periapical lesion. In Group 2, a submarginal incision was made. Before incision, the gingiva and mucosa were washed with 0.2% chlorhexidine gluconate. Bacterial samples were taken from the mucosa before reflecting the flap, and from the alveolar bone and the periapical lesion immediately after. All samples were cultured anaerobically on all-purpose and selective media. In Group 1, 12 of the 15 patients (80%) yielded bacteria from their mucosal samples despite the chlorhexidine wash. Bacterial growth was observed in all samples (100%) from the alveolar bone while the periapical lesions gave bacterial growth in 11 of 15 cases (73%). In Group 2, bacteria were cultured from the mucosa in 11 of 15 (73%) patients. Three samples (20%) from the alveolar bone and 10 from the periapical lesions (67%) gave positive growth. The predominant cultivable bacteria were anaerobic. Phenotypic profiling, performed with the data-based API bioMérieux system, indicated that the sampling technique used prevented mucosal bacteria from reaching the exposed bone and the periapical lesions. Profiling also indicated that following marginal incision, bacteria from the periodontal pocket might have reached the underlying tissues by surgeon-released bacteremia or direct translocation. Most organisms detected in the periapical lesions were clearly different from the bacteria present at neighboring sites and appeared to have been there before sampling.

Bacteria, Anaerobic↗
PubMed2000

Assessment of periradicular microbiota by DNA-DNA hybridization.

In the present study the "checkerboard" DNA-DNA hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. Thirty-four patients with root-filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. In Group 1, a marginal incision was performed during surgery to expose the lesion, and in Group 2, a submarginal incision was applied. The gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate solution prior to surgery. Bacterial DNA was identified in all samples from the two groups using 40 different whole genomic probes. The mean number (+/- SD) of species detected was 33.7 +/- 3.3 in Group 1 and 21.3 +/- 6.3 in Group 2 (P < 0.001). The majority of the probe-detected bacteria were present in more lesions from Group 1 than from Group 2. The differences were most notable for Campylobacter gracilis, Porphyromonas endodontalis, Propionibacterium acnes, Capnocytophaga gingivalis, Fusobacterium nucleatum ssp. nucleatum, Fusobacterium nucleatum ssp. polymorphum, Prevotella intermedia, Treponema denticola, Streptococcus constellatus and Actinomyces naeslundii I. Bacterial species such as Actinobacillus actinomycetemcomitans and Bacteroides forsythus were detected in more than 60% of the lesions from both groups. Also, P. endodontalis was abundant in periapical tissue. The data supported the idea that following a marginal incision, bacteria from the periodontal pocket might reach the underlying tissues by surgeon-released bacteremia. The study provided solid evidence that bacteria invade the periapical tissue of asymptomatic teeth with apical periodontitis. The detection of much more bacteria with the "checkerboard" DNA-DNA hybridization method than has previously been recovered by anaerobic culture indicated that the endodontic (and periodontal) microfloras should be redefined using molecular methods.

Actinomyces↗
PubMed1990

Desquamative gingivitis: preliminary observations with tetracycline treatment.

Fourteen patients admitted to our clinic were found to have desquamative gingivitis. They were treated with doxycycline monohydrate, a semisynthetic tetracycline, for 6 to 8 weeks. The gingival lesions were recorded photographically and were classified according to severity by means of a four-graded mucosal index system. Biopsies for histologic and immunohistochemical examination were taken initially. Symptoms and clinical changes of the mucosa were recorded during the treatment period. Initial and final mucosal index scores were compared. A statistically significant improvement was obtained after tetracycline treatment.

Aged↗
PubMed1988

Desquamative gingivitis responding to treatment with tetracycline: a pilot study.

We experienced remarkable regression of clinical signs and symptoms in three patients suffering from desquamative gingivitis, after treatment with 100 mg doxycycline monohydrate daily for 8 wk. The possible role of plaque substances as etiologic factors in the development or maintenance of desquamative gingivitis is postulated.

Dental Plaque↗
PubMed1988

Oral lichenoid reactions related to composite restorations. Preliminary report.

Lichenoid lesions topographically related to resin-based composite restorations were observed in 17 patients. In eight of these the composite had been inserted to replace amalgam restorations that were topographically related to lichenoid lesions. The other nine had no history of lichenoid lesions. Total remission occurred in four cases after the composite had been replaced with gold inlays or gold-porcelain crowns, and partial remission has been observed in five patients so far. The use of posterior composite restorations is rapidly increasing, and possible side effects, such as erosive lichenoid lesions, caused by these restorations should be considered in the differential diagnosis of lesions in the oral mucosa.

Adult↗
PubMed1987

Removal of third molar germs. Study of complications.

Enucleation of the third molar germ has been discussed as an alternative to extractions of premolars in borderline cases of crowding in the mandibular arch. A total of 86 patients in the age group 8-17 years had their mandibular third molar germs removed bilaterally by the same procedure in local anaesthesia. Intramuscular injection of 6 mg Celeston-Chronodose R was given postoperatively. We experienced that the postoperative complications were few and the psychological reactions were acceptable.

Adolescent↗
PubMed1987

Malignant transformation in oral leukoplakia.

A retrospective study on the incidence of malignant transformation in oral leukoplakia was performed covering the period from 1970 to 1980. Carcinoma developed in 11 of the 157 patients, and this 7% incidence increased to 8.9% when the same patients were followed prospectively for 6 more years. Dysplasia was found in biopsies from 51 patients (31.8%), and was significantly more frequent in women than men (P less than 0.05). Dysplasia grade and cancer development were related to updated clinical and etiological parameters.

Adult↗
PubMed1986

Local immunoreactivity and human papillomavirus (HPV) in oral precancer and cancer lesions.

A retrospective longitudinal study was performed on 20 patients with oral leukoplakia, 10 of which developed an oral squamous cell carcinoma, to assess whether any alterations in the local immunologic reactivity could be found of value in predicting the subsequent behavior of the lesions. During the major period of follow-up, the relative frequency of in situ IgA-producing plasma cells was significantly higher in biopsies from patients subsequently developing cancer than in patients showing no cancer development. Preceding the malignant transformation by 12 to 15 months, however, a remarkable shift from IgA to IgG plasma cell predominance was noticed in the biopsies of the cancer series, not detectable in the non-cancer group. HPV group specific capsid antigens were found in seven cases of the cancer series and in six of the non-cancer group. The possible diagnostic implications of these results are discussed.

Adult↗
PubMed1986

Amalgam-related oral lichenoid reaction.

In 52 patients with oral lichen planus topographically related to amalgam restorations, the fillings were replaced by other materials in 18, 16 of whom experienced complete remission of the lesions within 1-12 months. These results are discussed in relation to the results of epicutaneous patch tests for possible allergy to a number of mercury compounds. The term "oral lichenoid reaction", is suggested to describe these lesions.

Adult↗
PubMed1985

Malignant transformation in oral lichen planus.

A carcinoma arose in the buccal mucosa of a 71-year-old woman suffering from several systemic diseases, and on extensive medication. The buccal mucosa had been affected by oral lichen planus for more than 9 years. The possibility of malignant transformation of oral lichen planus is discussed.

Aged↗
PubMed1984

Electrogalvanically-induced contact allergy of the oral mucosa. Report of a case.

A 69-year-old white female presented bilateral lesions of the oral mucosa possibly related to electrogalvanism. The lesions were histologically characterized as lichen planus and as mild epithelial dysplasia on the left and right sides, respectively. They disappeared after removing amalgam restorations opposing the lesions. Epicutaneous patch tests and lymphocyte-transformation tests showed that the patient suffered from a contact allergy to mercury compounds, indicating this as a mechanism by which electrogalvanism may induce lesions of the oral mucosa.

Aged↗